Identification of a Sed5-like SNARE gene LjSYP32-1 that contributes to nodule tissue formation of Lotus japonicus

Ha Thu Mai, Mika Nomura, Kaoru Takegawa, Erika Asamizu, Syusei Sato, Tomohiko Kato, Satoshi Tabata, Shigeyuki Tajima

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We identified a Sed5-like clone LjSYP32-1 which contributes to nodule tissue formation and plant growth in Lotus japonicus. In the L. japonicus expressed sequence tag (EST) clone databases of Kazusa DNA Research Institute, another syntaxin-related clone (LjSYP32-2) was also detected, and the nucleotide and amino acid sequences of these two clone are very similar to each other. Real-time PCR and promoter analysis indicated that expression of LjSYP32-1 was dominant compared with LjSYP32-2 in the various plant organs. Promoter analysis and in situ hybridization revealed that LjSYP32-1 was expressed significantly in the inner cortex cell layer surrounding the infected zone of young nodules and in the meristem area of developing lateral root. To explore the function and physiological role of LjSYP32-1 in nodules and other plant organs, stable transformation lines of L. japonicus expressing either sense or antisense LjSYP32-1 were prepared. The antisense plants showed a significantly retarded plant growth phenotype, suggesting a role for LjSYP32-1 in supporting plant growth. In the same transgenic lines, the plants were capable of forming nodules, but the acetylene reduction activity was reduced by around 50% per plant. The nodules were much smaller and some nodules were fused to each other by sharing the inner cortex. The rate of occurence of such irregular nodules was twice that observed in wild-type plants. The data suggest that LjSYP32-1 contributes to the support of plant growth and normal nodule tissue differentiation.

Original languageEnglish
Pages (from-to)829-838
Number of pages10
JournalPlant and Cell Physiology
Volume47
Issue number7
DOIs
Publication statusPublished - Jul 1 2006

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SNARE Proteins
Lotus corniculatus var. japonicus
plant growth
clones
plant organs
Genes
cortex
genes
promoter regions
Clone Cells
acetylene reduction
expressed sequence tags
meristems
in situ hybridization
Growth
quantitative polymerase chain reaction
amino acid sequences
nucleotides
genetically modified organisms
phenotype

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

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Identification of a Sed5-like SNARE gene LjSYP32-1 that contributes to nodule tissue formation of Lotus japonicus. / Mai, Ha Thu; Nomura, Mika; Takegawa, Kaoru; Asamizu, Erika; Sato, Syusei; Kato, Tomohiko; Tabata, Satoshi; Tajima, Shigeyuki.

In: Plant and Cell Physiology, Vol. 47, No. 7, 01.07.2006, p. 829-838.

Research output: Contribution to journalArticle

Mai, Ha Thu ; Nomura, Mika ; Takegawa, Kaoru ; Asamizu, Erika ; Sato, Syusei ; Kato, Tomohiko ; Tabata, Satoshi ; Tajima, Shigeyuki. / Identification of a Sed5-like SNARE gene LjSYP32-1 that contributes to nodule tissue formation of Lotus japonicus. In: Plant and Cell Physiology. 2006 ; Vol. 47, No. 7. pp. 829-838.
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