TY - JOUR
T1 - Identification of ACE-inhibitory peptides in salt-free soy sauce that are transportable across caco-2 cell monolayers
AU - Zhu, Xiao Lin
AU - Watanabe, Keisuke
AU - Shiraishi, Koso
AU - Ueki, Tatsuro
AU - Noda, Yoshiharu
AU - Matsui, Toshiro
AU - Matsumoto, Kiyoshi
N1 - Funding Information:
This study was supported by a grant for the Project of Fukuoka Industry, Science and Technology Foundation (2006–2007) to T.M.
PY - 2008/3
Y1 - 2008/3
N2 - In present study, we aimed to identify angiotensin I-converting enzyme (ACE)-inhibitory peptides from a salt-free soy sauce (SFS), a newly developed antihypertensive seasoning obtained by Aspergillus oryzae fermentation of soybean in the absence of salt, which can be transported through caco-2 cell monolayers. Through an Ussing transport investigation of SFS across caco-2 cell monolayers, three di-peptides, Ala-Phe, Phe-Ile and Ile-Phe, were successfully identified from the SFS as transportable inhibitory peptides. Ala-Phe and Ile-Phe, but not Phe-Ile, exhibited ACE-inhibitory activity with IC50 values of 165.3 μM and 65.8 μM, respectively. Kinetic studies revealed that Ile-Phe (Km: 3.1 mM, Papp: 2.4 × 10-6 cm/s) exhibited greater affinity toward the transport compared with Ala-Phe (Km: 48.1 mM, Papp: 1.4 × 10-6 cm/s) and Phe-Ile (Km: 12.7 mM, Papp: 1.4 × 10-6 cm/s).
AB - In present study, we aimed to identify angiotensin I-converting enzyme (ACE)-inhibitory peptides from a salt-free soy sauce (SFS), a newly developed antihypertensive seasoning obtained by Aspergillus oryzae fermentation of soybean in the absence of salt, which can be transported through caco-2 cell monolayers. Through an Ussing transport investigation of SFS across caco-2 cell monolayers, three di-peptides, Ala-Phe, Phe-Ile and Ile-Phe, were successfully identified from the SFS as transportable inhibitory peptides. Ala-Phe and Ile-Phe, but not Phe-Ile, exhibited ACE-inhibitory activity with IC50 values of 165.3 μM and 65.8 μM, respectively. Kinetic studies revealed that Ile-Phe (Km: 3.1 mM, Papp: 2.4 × 10-6 cm/s) exhibited greater affinity toward the transport compared with Ala-Phe (Km: 48.1 mM, Papp: 1.4 × 10-6 cm/s) and Phe-Ile (Km: 12.7 mM, Papp: 1.4 × 10-6 cm/s).
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U2 - 10.1016/j.peptides.2007.11.006
DO - 10.1016/j.peptides.2007.11.006
M3 - Article
C2 - 18160180
AN - SCOPUS:39649089143
SN - 0196-9781
VL - 29
SP - 338
EP - 344
JO - Peptides
JF - Peptides
IS - 3
ER -