Identification of an hla-peptide complex contributing to japanese cedar pollinosis

Toshio Hori, Nobuhiro Kamikawaji, Akinori Kimura, Takehiko Sasazuki

Research output: Contribution to journalArticle

Abstract

We investigated the association between Japanese cedar pollinosis and HLA-class II alleles by HLA-DNA typing using a PCR-SSOP method and found that the frequency of HLA-DP5 (DPA 1*02022 and DPB 1*0501) was significantly increased in the patients. To investigate whether the HLA-DP5 was merely a genetic marker or rather involved in the pathogenesis of the disease, Japanese cedar pollen antigen (CPAg)-specific T cell lines were established from three patients who possessed HLA-DR4-DR53-DQ4-DP5 (DRB1 *0405-DRB4*01 -DQA1*0302-DQB1*0401 -DPA1*02022-DPB l *0501) haplotype. By using these CPAg-specific T cell lines and 88 synthesized overlapping peptides spanning the entire length of two major cedar pollen allergens, Cry j 1 and Cry j 2, two peptides recognized by DR53-restricted T cells and one peptide recognized by DPS-restricted T cells were identified. DPS-restricted T cell lines as well as DR53-restricted T cell lines produced Th2-type cytokines. Therefore, HLA-DP5 may be directly involved in the pathogenesis, by helping the IgE Ab production to CPAg.

Original languageEnglish
Number of pages1
JournalJapanese Journal of Human Genetics
Volume41
Issue number1
Publication statusPublished - Dec 1 1996

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Cryptomeria
Seasonal Allergic Rhinitis
Pollen
T-Lymphocytes
Peptides
Cell Line
Antigens
HLA-DR4 Antigen
Histocompatibility Testing
DNA Fingerprinting
Genetic Markers
Allergens
Haplotypes
Immunoglobulin E
Alleles
Cytokines
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Genetics(clinical)

Cite this

Identification of an hla-peptide complex contributing to japanese cedar pollinosis. / Hori, Toshio; Kamikawaji, Nobuhiro; Kimura, Akinori; Sasazuki, Takehiko.

In: Japanese Journal of Human Genetics, Vol. 41, No. 1, 01.12.1996.

Research output: Contribution to journalArticle

Hori, Toshio ; Kamikawaji, Nobuhiro ; Kimura, Akinori ; Sasazuki, Takehiko. / Identification of an hla-peptide complex contributing to japanese cedar pollinosis. In: Japanese Journal of Human Genetics. 1996 ; Vol. 41, No. 1.
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