Identification of an imprinted U2af binding protein related sequence on mouse chromosome 11 using the RLGS method

Y. Hayashizaki; H. Shibata; S. Hirotsune; H. Sugino; Y. Okazaki; N. Sasaki; K. Hirose; H. Imoto; H. Okuizumi; M. Muramatsu; H. Komatsubara; T. Shiroishi; K. Moriwaki; M. Katsuki; N. Hatano; H. Sasaki; T. Ueda; N. Mise; N. Takagi; C. Plass; V. M. Chapman

doi:10.1038/ng0194-33

Identification of an imprinted U2af binding protein related sequence on mouse chromosome 11 using the RLGS method

Y. Hayashizaki, H. Shibata, S. Hirotsune, H. Sugino, Y. Okazaki, N. Sasaki, K. Hirose, H. Imoto, H. Okuizumi, M. Muramatsu, H. Komatsubara, T. Shiroishi, K. Moriwaki, M. Katsuki, N. Hatano, H. Sasaki, T. Ueda, N. Mise, N. Takagi, C. PlassV. M. Chapman

Department of Molecular and Structural Biology

Research output: Contribution to journal › Article › peer-review

200 Citations (Scopus)

Abstract

A new imprinted gene has been discovered in mice using the technique of restriction landmark genomic scanning (RGLS) with methylation sensitive enzymes. Eight out of 3,100 strain–specific Notl and BssHII spots were identified as imprinted in reciprocal F₁ hybrids. Subsequently, we isolated a genomic clone for one locus on proximal chromosome 11 near the Glns locus, an imprinted region in uniparental disomic mice, and its corresponding cDNA clone. Expression of this transcript from the paternal allele was established using RT–PCR of reciprocal F₁–hybrid mice. The amino–acid sequence deduced from the cDNA showed significant homology to the U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit.

Original language	English
Pages (from-to)	33-40
Number of pages	8
Journal	Nature genetics
Volume	6
Issue number	1
DOIs	https://doi.org/10.1038/ng0194-33
Publication status	Published - Jan 1994

All Science Journal Classification (ASJC) codes

Genetics

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Hayashizaki, Y., Shibata, H., Hirotsune, S., Sugino, H., Okazaki, Y., Sasaki, N., Hirose, K., Imoto, H., Okuizumi, H., Muramatsu, M., Komatsubara, H., Shiroishi, T., Moriwaki, K., Katsuki, M., Hatano, N., Sasaki, H., Ueda, T., Mise, N., Takagi, N., ... Chapman, V. M. (1994). Identification of an imprinted U2af binding protein related sequence on mouse chromosome 11 using the RLGS method. Nature genetics, 6(1), 33-40. https://doi.org/10.1038/ng0194-33

Hayashizaki, Y, Shibata, H, Hirotsune, S, Sugino, H, Okazaki, Y, Sasaki, N, Hirose, K, Imoto, H, Okuizumi, H, Muramatsu, M, Komatsubara, H, Shiroishi, T, Moriwaki, K, Katsuki, M, Hatano, N, Sasaki, H, Ueda, T, Mise, N, Takagi, N, Plass, C & Chapman, VM 1994, 'Identification of an imprinted U2af binding protein related sequence on mouse chromosome 11 using the RLGS method', Nature genetics, vol. 6, no. 1, pp. 33-40. https://doi.org/10.1038/ng0194-33

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abstract = "A new imprinted gene has been discovered in mice using the technique of restriction landmark genomic scanning (RGLS) with methylation sensitive enzymes. Eight out of 3,100 strain–specific Notl and BssHII spots were identified as imprinted in reciprocal F1 hybrids. Subsequently, we isolated a genomic clone for one locus on proximal chromosome 11 near the Glns locus, an imprinted region in uniparental disomic mice, and its corresponding cDNA clone. Expression of this transcript from the paternal allele was established using RT–PCR of reciprocal F1–hybrid mice. The amino–acid sequence deduced from the cDNA showed significant homology to the U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit.",

author = "Y. Hayashizaki and H. Shibata and S. Hirotsune and H. Sugino and Y. Okazaki and N. Sasaki and K. Hirose and H. Imoto and H. Okuizumi and M. Muramatsu and H. Komatsubara and T. Shiroishi and K. Moriwaki and M. Katsuki and N. Hatano and H. Sasaki and T. Ueda and N. Mise and N. Takagi and C. Plass and Chapman, {V. M.}",

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AU - Shibata, H.

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AU - Sugino, H.

AU - Okazaki, Y.

AU - Sasaki, N.

AU - Hirose, K.

AU - Imoto, H.

AU - Okuizumi, H.

AU - Muramatsu, M.

AU - Komatsubara, H.

AU - Shiroishi, T.

AU - Moriwaki, K.

AU - Katsuki, M.

AU - Hatano, N.

AU - Sasaki, H.

AU - Ueda, T.

AU - Mise, N.

AU - Takagi, N.

AU - Plass, C.

AU - Chapman, V. M.

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Identification of an imprinted U2af binding protein related sequence on mouse chromosome 11 using the RLGS method

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