Identification of an inhibitor for interleukin 4-induced ε germline transcription and antigen-specific IgE production in vivo

Hirofumi Tachibana, Takao Kubo, Toshio Miyase, Sousuke Tanino, Miki Yoshimoto, Mitsuaki Sano, Mari Yamamoto-Maeda, Koji Yamada

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

IgE plays a key role in the pathogenesis of allergic disease. Interleukin (IL) 4 is a potent and critical stimulator of immunoglobulin class switching from IgM to IgE in B cells. IL-4 induces the expression of ε germline transcript (εGT), which is critical to initiate IgE production. While searching for molecules that inhibit εGT expression induced by IL-4, we found that polyphenol strictinin, which was isolated from tea leaves, was able to inhibit the IL-4-induced εGT expression in the human B cell line DND39. Strictinin also acted on human peripheral blood mononuclear cells obtained from healthy donors to inhibit IL-4-induced εGT expression. Strictinin demonstrated similar inhibitory activity in peripheral blood mononuclear cells obtained from atopic donors. Interestingly, strictinin decreased ovalbumin-induced IgE production in mice, whereas the production of IgG and IgM was not affected. Furthermore, we found that the IL-4-induced STAT6 tyrosine phosphorylation, which is essential for IL-4-induced εGT expression, was inhibited in DND39 cells upon treatment with strictinin. Taken together, these results suggest that strictinin can inhibit IgE production through the inhibition of IL-4-mediated signaling in B cells.

Original languageEnglish
Pages (from-to)53-60
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume280
Issue number1
DOIs
Publication statusPublished - Jan 1 2001

Fingerprint

Transcription
Interleukin-4
Immunoglobulin E
Antigens
B-Lymphocytes
Cells
Immunoglobulin M
Blood Cells
Blood
Immunoglobulin Class Switching
Phosphorylation
Immunoglobulin Isotypes
Ovalbumin
Polyphenols
Tea
Tyrosine
strictinin
Immunoglobulin G
Cell Line
Molecules

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Identification of an inhibitor for interleukin 4-induced ε germline transcription and antigen-specific IgE production in vivo. / Tachibana, Hirofumi; Kubo, Takao; Miyase, Toshio; Tanino, Sousuke; Yoshimoto, Miki; Sano, Mitsuaki; Yamamoto-Maeda, Mari; Yamada, Koji.

In: Biochemical and Biophysical Research Communications, Vol. 280, No. 1, 01.01.2001, p. 53-60.

Research output: Contribution to journalArticle

Tachibana, Hirofumi ; Kubo, Takao ; Miyase, Toshio ; Tanino, Sousuke ; Yoshimoto, Miki ; Sano, Mitsuaki ; Yamamoto-Maeda, Mari ; Yamada, Koji. / Identification of an inhibitor for interleukin 4-induced ε germline transcription and antigen-specific IgE production in vivo. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 280, No. 1. pp. 53-60.
@article{9e15c1a1ef8e4ae7892283b57759153d,
title = "Identification of an inhibitor for interleukin 4-induced ε germline transcription and antigen-specific IgE production in vivo",
abstract = "IgE plays a key role in the pathogenesis of allergic disease. Interleukin (IL) 4 is a potent and critical stimulator of immunoglobulin class switching from IgM to IgE in B cells. IL-4 induces the expression of ε germline transcript (εGT), which is critical to initiate IgE production. While searching for molecules that inhibit εGT expression induced by IL-4, we found that polyphenol strictinin, which was isolated from tea leaves, was able to inhibit the IL-4-induced εGT expression in the human B cell line DND39. Strictinin also acted on human peripheral blood mononuclear cells obtained from healthy donors to inhibit IL-4-induced εGT expression. Strictinin demonstrated similar inhibitory activity in peripheral blood mononuclear cells obtained from atopic donors. Interestingly, strictinin decreased ovalbumin-induced IgE production in mice, whereas the production of IgG and IgM was not affected. Furthermore, we found that the IL-4-induced STAT6 tyrosine phosphorylation, which is essential for IL-4-induced εGT expression, was inhibited in DND39 cells upon treatment with strictinin. Taken together, these results suggest that strictinin can inhibit IgE production through the inhibition of IL-4-mediated signaling in B cells.",
author = "Hirofumi Tachibana and Takao Kubo and Toshio Miyase and Sousuke Tanino and Miki Yoshimoto and Mitsuaki Sano and Mari Yamamoto-Maeda and Koji Yamada",
year = "2001",
month = "1",
day = "1",
doi = "10.1006/bbrc.2000.4069",
language = "English",
volume = "280",
pages = "53--60",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Identification of an inhibitor for interleukin 4-induced ε germline transcription and antigen-specific IgE production in vivo

AU - Tachibana, Hirofumi

AU - Kubo, Takao

AU - Miyase, Toshio

AU - Tanino, Sousuke

AU - Yoshimoto, Miki

AU - Sano, Mitsuaki

AU - Yamamoto-Maeda, Mari

AU - Yamada, Koji

PY - 2001/1/1

Y1 - 2001/1/1

N2 - IgE plays a key role in the pathogenesis of allergic disease. Interleukin (IL) 4 is a potent and critical stimulator of immunoglobulin class switching from IgM to IgE in B cells. IL-4 induces the expression of ε germline transcript (εGT), which is critical to initiate IgE production. While searching for molecules that inhibit εGT expression induced by IL-4, we found that polyphenol strictinin, which was isolated from tea leaves, was able to inhibit the IL-4-induced εGT expression in the human B cell line DND39. Strictinin also acted on human peripheral blood mononuclear cells obtained from healthy donors to inhibit IL-4-induced εGT expression. Strictinin demonstrated similar inhibitory activity in peripheral blood mononuclear cells obtained from atopic donors. Interestingly, strictinin decreased ovalbumin-induced IgE production in mice, whereas the production of IgG and IgM was not affected. Furthermore, we found that the IL-4-induced STAT6 tyrosine phosphorylation, which is essential for IL-4-induced εGT expression, was inhibited in DND39 cells upon treatment with strictinin. Taken together, these results suggest that strictinin can inhibit IgE production through the inhibition of IL-4-mediated signaling in B cells.

AB - IgE plays a key role in the pathogenesis of allergic disease. Interleukin (IL) 4 is a potent and critical stimulator of immunoglobulin class switching from IgM to IgE in B cells. IL-4 induces the expression of ε germline transcript (εGT), which is critical to initiate IgE production. While searching for molecules that inhibit εGT expression induced by IL-4, we found that polyphenol strictinin, which was isolated from tea leaves, was able to inhibit the IL-4-induced εGT expression in the human B cell line DND39. Strictinin also acted on human peripheral blood mononuclear cells obtained from healthy donors to inhibit IL-4-induced εGT expression. Strictinin demonstrated similar inhibitory activity in peripheral blood mononuclear cells obtained from atopic donors. Interestingly, strictinin decreased ovalbumin-induced IgE production in mice, whereas the production of IgG and IgM was not affected. Furthermore, we found that the IL-4-induced STAT6 tyrosine phosphorylation, which is essential for IL-4-induced εGT expression, was inhibited in DND39 cells upon treatment with strictinin. Taken together, these results suggest that strictinin can inhibit IgE production through the inhibition of IL-4-mediated signaling in B cells.

UR - http://www.scopus.com/inward/record.url?scp=0034810724&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034810724&partnerID=8YFLogxK

U2 - 10.1006/bbrc.2000.4069

DO - 10.1006/bbrc.2000.4069

M3 - Article

C2 - 11162477

AN - SCOPUS:0034810724

VL - 280

SP - 53

EP - 60

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -