Identification of Cannabis pollens using an allergic patient's immunoglobulin E and purification and characterization of allergens in Cannabis pollens

Hiroyuki Tanaka, Mie Degawa, Etsuo Kawata, Jun Hayashi, Yukihiro Shoyama

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Cannabis pollen allergens were detected using the serum of an allergic patient. The allergens were then purified by sequential column chromatography (including DE52 cellulose and phenyl-Sepharose CL-4B) and preparative HPLC. The molecular weight of the allergens were determined as 10 050 and 13 706 by matrix-assisted laser desorption/ionization time of flight mass spectrometry. We utilised Western blotting and development of an enzyme-linked immunosorbent assay for the detection of Cannabis pollen allergens. Copyright (C) 1998 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)139-153
Number of pages15
JournalForensic Science International
Issue number2-3
Publication statusPublished - Nov 9 1998


All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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