Identification of Cannabis pollens using an allergic patient's immunoglobulin E and purification and characterization of allergens in Cannabis pollens

Hiroyuki Tanaka; Mie Degawa; Etsuo Kawata; Jun Hayashi; Yukihiro Shoyama

doi:10.1016/S0379-0738(98)00152-2

Identification of Cannabis pollens using an allergic patient's immunoglobulin E and purification and characterization of allergens in Cannabis pollens

Hiroyuki Tanaka, Mie Degawa, Etsuo Kawata, Jun Hayashi, Yukihiro Shoyama

Pharmaceutical Health Care and Sciences

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Cannabis pollen allergens were detected using the serum of an allergic patient. The allergens were then purified by sequential column chromatography (including DE52 cellulose and phenyl-Sepharose CL-4B) and preparative HPLC. The molecular weight of the allergens were determined as 10 050 and 13 706 by matrix-assisted laser desorption/ionization time of flight mass spectrometry. We utilised Western blotting and development of an enzyme-linked immunosorbent assay for the detection of Cannabis pollen allergens. Copyright (C) 1998 Elsevier Science Ireland Ltd.

Original language	English
Pages (from-to)	139-153
Number of pages	15
Journal	Forensic Science International
Volume	97
Issue number	2-3
DOIs	https://doi.org/10.1016/S0379-0738(98)00152-2
Publication status	Published - Nov 9 1998

All Science Journal Classification (ASJC) codes

Pathology and Forensic Medicine

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10.1016/S0379-0738(98)00152-2

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AU - Degawa, Mie

AU - Kawata, Etsuo

AU - Hayashi, Jun

AU - Shoyama, Yukihiro

PY - 1998/11/9

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AB - Cannabis pollen allergens were detected using the serum of an allergic patient. The allergens were then purified by sequential column chromatography (including DE52 cellulose and phenyl-Sepharose CL-4B) and preparative HPLC. The molecular weight of the allergens were determined as 10 050 and 13 706 by matrix-assisted laser desorption/ionization time of flight mass spectrometry. We utilised Western blotting and development of an enzyme-linked immunosorbent assay for the detection of Cannabis pollen allergens. Copyright (C) 1998 Elsevier Science Ireland Ltd.

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Identification of Cannabis pollens using an allergic patient's immunoglobulin E and purification and characterization of allergens in Cannabis pollens

Abstract

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