Identification of Cannabis pollens using an allergic patient's immunoglobulin E and purification and characterization of allergens in Cannabis pollens

Hiroyuki Tanaka, Mie Degawa, Etsuo Kawata, Jun Hayashi, Yukihiro Shoyama

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Cannabis pollen allergens were detected using the serum of an allergic patient. The allergens were then purified by sequential column chromatography (including DE52 cellulose and phenyl-Sepharose CL-4B) and preparative HPLC. The molecular weight of the allergens were determined as 10 050 and 13 706 by matrix-assisted laser desorption/ionization time of flight mass spectrometry. We utilised Western blotting and development of an enzyme-linked immunosorbent assay for the detection of Cannabis pollen allergens. Copyright (C) 1998 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)139-153
Number of pages15
JournalForensic Science International
Volume97
Issue number2-3
DOIs
Publication statusPublished - Nov 9 1998

Fingerprint

Cannabis
Pollen
Allergens
Immunoglobulin E
Cellulose
Chromatography
Mass Spectrometry
Lasers
Molecular Weight
Western Blotting
Enzyme-Linked Immunosorbent Assay
High Pressure Liquid Chromatography
Serum

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Identification of Cannabis pollens using an allergic patient's immunoglobulin E and purification and characterization of allergens in Cannabis pollens. / Tanaka, Hiroyuki; Degawa, Mie; Kawata, Etsuo; Hayashi, Jun; Shoyama, Yukihiro.

In: Forensic Science International, Vol. 97, No. 2-3, 09.11.1998, p. 139-153.

Research output: Contribution to journalArticle

Tanaka, Hiroyuki ; Degawa, Mie ; Kawata, Etsuo ; Hayashi, Jun ; Shoyama, Yukihiro. / Identification of Cannabis pollens using an allergic patient's immunoglobulin E and purification and characterization of allergens in Cannabis pollens. In: Forensic Science International. 1998 ; Vol. 97, No. 2-3. pp. 139-153.
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