Identification of cDNAs encoding the WD repeat protein and auxin-regulated protein in nicotiana glutinosa leaves induced in response to tobacco mosaic virus-infection at 25°C

Masumi Abe, Takeshi Hayashi, Kazumasa Hada, Makoto Kimura

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Abstract

Nicotiana glutinosa represents resistance to tobacco mosaic virus (TMV)- infection by causing a localized hypersensitive response (HR) at 25 °C. The fluorescent differential display (FDD) analysis followed by reverse transcription (RT)-PCR indicated that two transcripts encoding a WD-repeated protein and a putative auxin-regulated protein were significantly increased upon TMV-infection at 25 °C. The two fulllength cDNAs (Ng0881 and Ngl851), which encode the WD repeat protein and auxin-regulated protein, were cloned, and the complete nucleotide sequences were determined. The deduced amino acid sequence for Ng0881 has seven repetitive WD repeats, sharing 81% identical residues with the homologue from Oryza sativa, while that for Ng1851 shares 68% identical residues with the auxin-regulated protein from Oryza sativa. It could be speculated that translational products of the genes ng0881 and ng1851 may serve as signal mediators required for TMV-resistance.

Original languageEnglish
Pages (from-to)459-465
Number of pages7
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume53
Issue number2
Publication statusPublished - Oct 1 2008

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

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