Identification of CEACAM6 as an intermediate filament-associated protein expressed in sertoli cells of rat testis

Hitoshi Kurio, Hiroshi Hatsuda, Emi Murayama, Takane Kaneko, Hiroshi Iida

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Ceacam6 (carcinoembryonic antigen-related cell adhesion molecule 6 gene) has recently been isolated by differential display followed by RT-PCR and DNA sequence analyses. Ceacam6 is a member of an immunoglobulin superfamily and encodes a protein of 266 amino acid residues possessing one immunoglobulin (Ig)-like domain. RT-PCR analysis showed that Ceacam6 was dominantly expressed in rat testis and its expression level prominently increased after 6 wk of postnatal development in testis. Immunohistochemical analyses using the anti-CEACAM6 antibody revealed that CEACAM6 colocalized with intermediate filaments (vimentin) in Sertoli cells and interstitial cells. The association between CEACAM6 and vimentin was observed throughout postnatal development in rat testis. Transfection experiments performed in COS-7 cells suggested that overexpression of CEACAM6 brought about aggregation of vimentin filament around nuclei with which CEACAM6 colocalized and that the N-terminus region of CEACAM6, including the Ig-like domain, seemed to be required for association with vimentin filaments. Interaction between CEACAM6 and vimentin in rat testis and transfected COS-7 cells was confirmed by immunoprecipitation. Our observations strongly suggested that CEACAM6 might be a novel intermediate filament-associated protein involved in regulation of vimentin architecture in Sertoli cells.

Original languageEnglish
Pages (from-to)924-933
Number of pages10
JournalBiology of Reproduction
Volume85
Issue number5
DOIs
Publication statusPublished - Nov 1 2011

Fingerprint

Intermediate Filament Proteins
Sertoli Cells
Vimentin
Testis
Carcinoembryonic Antigen
Cell Adhesion Molecules
COS Cells
Genes
Polymerase Chain Reaction
Intermediate Filaments
DNA Sequence Analysis
Immunoprecipitation
Transfection
Immunoglobulins
Anti-Idiotypic Antibodies
Amino Acids
Proteins

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Cell Biology

Cite this

Identification of CEACAM6 as an intermediate filament-associated protein expressed in sertoli cells of rat testis. / Kurio, Hitoshi; Hatsuda, Hiroshi; Murayama, Emi; Kaneko, Takane; Iida, Hiroshi.

In: Biology of Reproduction, Vol. 85, No. 5, 01.11.2011, p. 924-933.

Research output: Contribution to journalArticle

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