Identification of DNA-Binding Proteins Changed after Induction of Sporulation in Bacillus Cereus

Kiyoshi Matsuno, Takahisa Miyamoto, Ken Ichiro Yamaguchi, Md Abu Sayed, Taishin Kajiwara, Shoji Hatano

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

DNA-binding proteins were extracted from both exponentially growing cells of Bacillus cereus ts-4 and cells that were induced to sporulate at different stages of chromosome replication, by using a double-stranded B. cereus ts-4 DNA-cellulose column. Two-dimensional electrophoresis of the proteins found that the amounts of 17 proteins changed drastically after induction of sporulation at all the stages. For 8 of those proteins, the largest or the smallest amount was found in the cells which were induced to sporulate 40 min after the initiation of chromosome replication, the sensitive stage for sporulation. The N-terminal amino acids of 6 proteins among the selected proteins were sequenced. The sequence obtained from a 59-kDa protein had sequence similarity (> 45%) to GroEL from several bacterial species. In addition, the sequences from 76- and 52-kDa proteins matched deduced amino acid sequences of a Mycobacterium leprae gene showing homology to the bacterial atp operon and the B. subtilis guaB for IMP dehydrogenase, respectively.

Original languageEnglish
Pages (from-to)231-235
Number of pages5
JournalBioscience, biotechnology, and biochemistry
Volume59
Issue number2
DOIs
Publication statusPublished - Jan 1 1995

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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