Abstract
During food shortage, organisms activate defense mechanisms to maximize their chance of survival. At least in part, these responses are triggered by changes in hormonal status and neural status during starvation. The hypothalamus is organized as a collection of distinct autonomously active nuclei and is considered to play crucial roles in these survival responses. To isolate factors involved in these pathways, we carried out suppression subtractive hybridization analyses using complementary DNAs (cDNA) from the hypothalami of fasted and fed rats. We identified four genes, namely ubiquitin-conjugating enzyme E2D 3 (UBE2D3), cAMP-dependent protein kinase C beta subunit (PKCβ), excitatory amino acid carrier 1 (EAAC1), and ferritin heavy polypeptide 1 (Fth1), that were upregulated after a 48-h fast compared to the fed status. According to previous reports, these genes have been implicated in protection against neuronal cell death under various neurodegenerative stresses, such as hypoxia-ischemia and oxidative stress. Thus, the increased expressions of the genes identified in the present study may have protective effects against neural damage that could otherwise result in cell death.
| Original language | English |
|---|---|
| Pages (from-to) | 216-226 |
| Number of pages | 11 |
| Journal | Annals of the New York Academy of Sciences |
| Volume | 1119 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2007 |
| Externally published | Yes |
Fingerprint
All Science Journal Classification (ASJC) codes
- Neuroscience(all)
- Biochemistry, Genetics and Molecular Biology(all)
- History and Philosophy of Science
Cite this
Identification of fasting-induced genes in the rat hypothalamus : Relationship with neuroprotection. / Chiba, Takuya; Fujita, Satoshi; Kubota, Haruaki; Inoue, Daisuke; Mizuno, Aya; Komatsu, Toshimitsu; Yamaza, Haruyoshi; Higami, Yoshikazu; Shimokawa, Isao.
In: Annals of the New York Academy of Sciences, Vol. 1119, No. 1, 01.01.2007, p. 216-226.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of fasting-induced genes in the rat hypothalamus
T2 - Relationship with neuroprotection
AU - Chiba, Takuya
AU - Fujita, Satoshi
AU - Kubota, Haruaki
AU - Inoue, Daisuke
AU - Mizuno, Aya
AU - Komatsu, Toshimitsu
AU - Yamaza, Haruyoshi
AU - Higami, Yoshikazu
AU - Shimokawa, Isao
PY - 2007/1/1
Y1 - 2007/1/1
N2 - During food shortage, organisms activate defense mechanisms to maximize their chance of survival. At least in part, these responses are triggered by changes in hormonal status and neural status during starvation. The hypothalamus is organized as a collection of distinct autonomously active nuclei and is considered to play crucial roles in these survival responses. To isolate factors involved in these pathways, we carried out suppression subtractive hybridization analyses using complementary DNAs (cDNA) from the hypothalami of fasted and fed rats. We identified four genes, namely ubiquitin-conjugating enzyme E2D 3 (UBE2D3), cAMP-dependent protein kinase C beta subunit (PKCβ), excitatory amino acid carrier 1 (EAAC1), and ferritin heavy polypeptide 1 (Fth1), that were upregulated after a 48-h fast compared to the fed status. According to previous reports, these genes have been implicated in protection against neuronal cell death under various neurodegenerative stresses, such as hypoxia-ischemia and oxidative stress. Thus, the increased expressions of the genes identified in the present study may have protective effects against neural damage that could otherwise result in cell death.
AB - During food shortage, organisms activate defense mechanisms to maximize their chance of survival. At least in part, these responses are triggered by changes in hormonal status and neural status during starvation. The hypothalamus is organized as a collection of distinct autonomously active nuclei and is considered to play crucial roles in these survival responses. To isolate factors involved in these pathways, we carried out suppression subtractive hybridization analyses using complementary DNAs (cDNA) from the hypothalami of fasted and fed rats. We identified four genes, namely ubiquitin-conjugating enzyme E2D 3 (UBE2D3), cAMP-dependent protein kinase C beta subunit (PKCβ), excitatory amino acid carrier 1 (EAAC1), and ferritin heavy polypeptide 1 (Fth1), that were upregulated after a 48-h fast compared to the fed status. According to previous reports, these genes have been implicated in protection against neuronal cell death under various neurodegenerative stresses, such as hypoxia-ischemia and oxidative stress. Thus, the increased expressions of the genes identified in the present study may have protective effects against neural damage that could otherwise result in cell death.
UR - http://www.scopus.com/inward/record.url?scp=36549034330&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36549034330&partnerID=8YFLogxK
U2 - 10.1196/annals.1404.013
DO - 10.1196/annals.1404.013
M3 - Article
C2 - 18056970
AN - SCOPUS:36549034330
VL - 1119
SP - 216
EP - 226
JO - Annals of the New York Academy of Sciences
JF - Annals of the New York Academy of Sciences
SN - 0077-8923
IS - 1
ER -