Identification of FOXC1 as a TGF-β1 responsive gene and its involvement in negative regulation of cell growth

Yong Zhou, Hidenori Kato, Kazuo Asanoma, Haruhiko Kondo, Takahiro Arima, Kiyoko Kato, Takao Matsuda, Norio Wake

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

We cloned the forkhead box C1 (FOXC1) gene, a member of the forkhead/winged-helix transcription factor family, as a transforming growth factor-β1 (TGF-β1) responsive gene. We showed that TGF-β1 upregulated transcription of FOXC1 in several human cancer cell lines. Ectopic expression of FOXC1 cDNA in HeLa cells, which lack both copies of the FOXC1 allele, restores the potential of TGF-β1 to inhibit cell growth by arresting cells in the G0/G1 phase. In addition, screens of primary endometrial and ovarian cancers revealed homozygous deletion of FOXC1 in 6.7% of them, one nonsense and one missense mutation of FOXC1, and transcriptional silencing in 11.7% of primary cancers. Evidence that a significant fraction of primary cancers exhibited somatic mutations suggests that FOXC1 functions as a tumor suppressor through TGF-β1 mediated signals.

Original languageEnglish
Pages (from-to)465-472
Number of pages8
JournalGenomics
Volume80
Issue number5
DOIs
Publication statusPublished - Jan 1 2002

All Science Journal Classification (ASJC) codes

  • Genetics

Fingerprint Dive into the research topics of 'Identification of FOXC1 as a TGF-β1 responsive gene and its involvement in negative regulation of cell growth'. Together they form a unique fingerprint.

  • Cite this