Identification of genes encoding cement-like antigens expressed in the salivary glands of Haemaphysalis longicornis

Thasaneeya Harnnoi, Takeshi Sakaguchi, Xuenan Xuan, Kozo Fujisaki

Research output: Contribution to journalArticlepeer-review

Abstract

A cDNA expression library from the salivary glands of hard tick, Haemaphysalis longicornis, was constructed. Immunoscreening was performed using sera of the rabbit repeatedly infested with ticks and seventeen positive clones were obtained. A BLASTP search suggested that 8 sequences matched with that of hypothetical H. longicornis sequence and one clone encoded HL35 antigen U from the same tick species. Eight of 17 gave no match to any sequence reported in the database. The proteins expected from these novel sequences possess common characteristics with cement proteins which assist ticks in their attachment to the host during blood feeding. The expression of these genes in salivary glands was confirmed by RT-PCR. Four of the 8 sequences showed to be upregulated upon blood feeding. These immunodominant antigens are of particular interest as candidates for future cement protein based-tick vaccine.

Original languageEnglish
Pages (from-to)1155-60
Number of pages6
JournalJournal of Veterinary Medical Science
Volume68
Issue number11
Publication statusPublished - Nov 2006

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