Identification of genes involved in the suppression of antibody production from human peripheral blood lymphocytes

Yoshihiro Aiba; Makiko Yamashita; Yoshinori Katakura; Yuki Furukawa; Shin Ei Matsumoto; Kousuke Tomimatsu; Kiichiro Teruya; Sanetaka Shirahata

doi:10.1271/bbb.70.966

Identification of genes involved in the suppression of antibody production from human peripheral blood lymphocytes

Yoshihiro Aiba, Makiko Yamashita, Yoshinori Katakura, Yuki Furukawa, Shin Ei Matsumoto, Kousuke Tomimatsu, Kiichiro Teruya, Sanetaka Shirahata

Systems Biology

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Pretreatment with L-leucyl-L-leucine methyl ester (LLME) is a prerequisite for peripheral blood mononuclear cells (PBMCs) to produce antigen-specific antibodies when sensitized with an antigen. Little information, however, is available regarding the mechanisms involved in LLME-induced augmentation of antibody production from PBMCs that are antigen sensitized. In the present study, we attempted to identify the genes involved in the suppression of antibody production from PBMCs that was not treated with LLME, but sensitized with an antigen. Using subtractive screening, we obtained 63 independent genes, including 17 EST genes, that are specific for LLME-nontreated PBMC. Among these genes, the expression of heavy chain ferritin (H-ferritin), CC chemokine ligand 18 (CCL18), and matrix metalloproteinase 12 (MMP12) were augmented in LLME-nontreated PBMCs, suggesting that inflammatory factors might be involved in the suppression of antibody production in LLME-nontreated PBMCs.

Original language	English
Pages (from-to)	966-970
Number of pages	5
Journal	Bioscience, Biotechnology and Biochemistry
Volume	70
Issue number	4
DOIs	https://doi.org/10.1271/bbb.70.966
Publication status	Published - 2006

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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Identification of genes involved in the suppression of antibody production from human peripheral blood lymphocytes. / Aiba, Yoshihiro; Yamashita, Makiko; Katakura, Yoshinori; Furukawa, Yuki; Matsumoto, Shin Ei; Tomimatsu, Kousuke; Teruya, Kiichiro; Shirahata, Sanetaka.

In: Bioscience, Biotechnology and Biochemistry, Vol. 70, No. 4, 2006, p. 966-970.

Research output: Contribution to journal › Article › peer-review

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abstract = "Pretreatment with L-leucyl-L-leucine methyl ester (LLME) is a prerequisite for peripheral blood mononuclear cells (PBMCs) to produce antigen-specific antibodies when sensitized with an antigen. Little information, however, is available regarding the mechanisms involved in LLME-induced augmentation of antibody production from PBMCs that are antigen sensitized. In the present study, we attempted to identify the genes involved in the suppression of antibody production from PBMCs that was not treated with LLME, but sensitized with an antigen. Using subtractive screening, we obtained 63 independent genes, including 17 EST genes, that are specific for LLME-nontreated PBMC. Among these genes, the expression of heavy chain ferritin (H-ferritin), CC chemokine ligand 18 (CCL18), and matrix metalloproteinase 12 (MMP12) were augmented in LLME-nontreated PBMCs, suggesting that inflammatory factors might be involved in the suppression of antibody production in LLME-nontreated PBMCs.",

author = "Yoshihiro Aiba and Makiko Yamashita and Yoshinori Katakura and Yuki Furukawa and Matsumoto, {Shin Ei} and Kousuke Tomimatsu and Kiichiro Teruya and Sanetaka Shirahata",

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AU - Katakura, Yoshinori

AU - Furukawa, Yuki

AU - Matsumoto, Shin Ei

AU - Tomimatsu, Kousuke

AU - Teruya, Kiichiro

AU - Shirahata, Sanetaka

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