Identification of genes involved in the suppression of antibody production from human peripheral blood lymphocytes

Yoshihiro Aiba, Makiko Yamashita, Yoshinori Katakura, Yuki Furukawa, Shin Ei Matsumoto, Kousuke Tomimatsu, Kiichiro Teruya, Sanetaka Shirahata

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Pretreatment with L-leucyl-L-leucine methyl ester (LLME) is a prerequisite for peripheral blood mononuclear cells (PBMCs) to produce antigen-specific antibodies when sensitized with an antigen. Little information, however, is available regarding the mechanisms involved in LLME-induced augmentation of antibody production from PBMCs that are antigen sensitized. In the present study, we attempted to identify the genes involved in the suppression of antibody production from PBMCs that was not treated with LLME, but sensitized with an antigen. Using subtractive screening, we obtained 63 independent genes, including 17 EST genes, that are specific for LLME-nontreated PBMC. Among these genes, the expression of heavy chain ferritin (H-ferritin), CC chemokine ligand 18 (CCL18), and matrix metalloproteinase 12 (MMP12) were augmented in LLME-nontreated PBMCs, suggesting that inflammatory factors might be involved in the suppression of antibody production in LLME-nontreated PBMCs.

Original languageEnglish
Pages (from-to)966-970
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume70
Issue number4
DOIs
Publication statusPublished - 2006

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Identification of genes involved in the suppression of antibody production from human peripheral blood lymphocytes'. Together they form a unique fingerprint.

Cite this