Abstract
Pretreatment with L-leucyl-L-leucine methyl ester (LLME) is a prerequisite for peripheral blood mononuclear cells (PBMCs) to produce antigen-specific antibodies when sensitized with an antigen. Little information, however, is available regarding the mechanisms involved in LLME-induced augmentation of antibody production from PBMCs that are antigen sensitized. In the present study, we attempted to identify the genes involved in the suppression of antibody production from PBMCs that was not treated with LLME, but sensitized with an antigen. Using subtractive screening, we obtained 63 independent genes, including 17 EST genes, that are specific for LLME-nontreated PBMC. Among these genes, the expression of heavy chain ferritin (H-ferritin), CC chemokine ligand 18 (CCL18), and matrix metalloproteinase 12 (MMP12) were augmented in LLME-nontreated PBMCs, suggesting that inflammatory factors might be involved in the suppression of antibody production in LLME-nontreated PBMCs.
| Original language | English |
|---|---|
| Pages (from-to) | 966-970 |
| Number of pages | 5 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 70 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - May 5 2006 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry
Cite this
Identification of genes involved in the suppression of antibody production from human peripheral blood lymphocytes. / Aiba, Yoshihiro; Yamashita, Makiko; Katakura, Yoshinori; Furukawa, Yuki; Matsumoto, Shin Ei; Tomimatsu, Kousuke; Teruya, Kiichiro; Shirahata, Sanetaka.
In: Bioscience, Biotechnology and Biochemistry, Vol. 70, No. 4, 05.05.2006, p. 966-970.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of genes involved in the suppression of antibody production from human peripheral blood lymphocytes
AU - Aiba, Yoshihiro
AU - Yamashita, Makiko
AU - Katakura, Yoshinori
AU - Furukawa, Yuki
AU - Matsumoto, Shin Ei
AU - Tomimatsu, Kousuke
AU - Teruya, Kiichiro
AU - Shirahata, Sanetaka
PY - 2006/5/5
Y1 - 2006/5/5
N2 - Pretreatment with L-leucyl-L-leucine methyl ester (LLME) is a prerequisite for peripheral blood mononuclear cells (PBMCs) to produce antigen-specific antibodies when sensitized with an antigen. Little information, however, is available regarding the mechanisms involved in LLME-induced augmentation of antibody production from PBMCs that are antigen sensitized. In the present study, we attempted to identify the genes involved in the suppression of antibody production from PBMCs that was not treated with LLME, but sensitized with an antigen. Using subtractive screening, we obtained 63 independent genes, including 17 EST genes, that are specific for LLME-nontreated PBMC. Among these genes, the expression of heavy chain ferritin (H-ferritin), CC chemokine ligand 18 (CCL18), and matrix metalloproteinase 12 (MMP12) were augmented in LLME-nontreated PBMCs, suggesting that inflammatory factors might be involved in the suppression of antibody production in LLME-nontreated PBMCs.
AB - Pretreatment with L-leucyl-L-leucine methyl ester (LLME) is a prerequisite for peripheral blood mononuclear cells (PBMCs) to produce antigen-specific antibodies when sensitized with an antigen. Little information, however, is available regarding the mechanisms involved in LLME-induced augmentation of antibody production from PBMCs that are antigen sensitized. In the present study, we attempted to identify the genes involved in the suppression of antibody production from PBMCs that was not treated with LLME, but sensitized with an antigen. Using subtractive screening, we obtained 63 independent genes, including 17 EST genes, that are specific for LLME-nontreated PBMC. Among these genes, the expression of heavy chain ferritin (H-ferritin), CC chemokine ligand 18 (CCL18), and matrix metalloproteinase 12 (MMP12) were augmented in LLME-nontreated PBMCs, suggesting that inflammatory factors might be involved in the suppression of antibody production in LLME-nontreated PBMCs.
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UR - http://www.scopus.com/inward/citedby.url?scp=33646158687&partnerID=8YFLogxK
U2 - 10.1271/bbb.70.966
DO - 10.1271/bbb.70.966
M3 - Article
C2 - 16636465
AN - SCOPUS:33646158687
VL - 70
SP - 966
EP - 970
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 4
ER -