Identification of Grf1 on mouse chromosome 9 as an imprinted gene by RLGS-M

C. Plass, H. Shibata, I. Kalcheva, L. Mullins, N. Kotelevtseva, J. Mullins, R. Kato, H. Sasaki, S. Hirotsune, Y. Okazaki, W. A. Held, Y. Hayashizaki, V. M. Chapman

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

Normal mammalian development requires a diploid combination of both haploid parental genomes. Uniparental disomy for certain segments of specific chromosomes results in aberrant development or prenatal lethality, indicating that the parental genomes have undergone modifications during gametogenesis. These modifications result in parent-of-origin specific expression for some genes, a phenomenon called genomic imprinting. Recent work with DNA methyltransferase deficient mice showed that differential methylation is the probable basis of imprinted character of several genes. Screening for endogenous imprinted loci using restriction landmark genomic scanning with methylation sensitive enzymes (RLGS-M) identified eight imprinted RLGS (Irlgs) candidate loci. Molecular analysis of the genomic region of one of the loci (Irlgs2) resulted in the discovery of the paternally imprinted U2afbp-rs gene within a previously identified imprinted region on mouse chromosome 11 (refs 5, 7). This paper describes the characterisation of a novel imprinted RLGS-M locus, Irlgs3, on mouse chromosome 9 (ref. 6). Within this locus we identified the Grf1 (also called Cdc25(Mm)) gene, which is homologous to the RAS-specific guanine nucleotide exchange factor gene, CDC25, in Saccharomyces cerevisiae. Grf1 is located about 30 kb downstream of the methylation imprinted site, identified by RLGS-M, and shows paternal allele specific expression in mouse brain, stomach and heart. Our results indicate that imprinting may have a role in regulating mitogenic signal transduction pathways during growth and development.

Original languageEnglish
Pages (from-to)106-109
Number of pages4
JournalNature genetics
Volume14
Issue number1
DOIs
Publication statusPublished - Sep 23 1996
Externally publishedYes

Fingerprint

Chromosomes, Human, Pair 9
Methylation
Genes
Minor Lymphocyte Stimulatory Loci
Uniparental Disomy
Genome
Gametogenesis
Genomic Imprinting
Guanine Nucleotide Exchange Factors
Chromosomes, Human, Pair 11
Haploidy
Methyltransferases
Diploidy
Growth and Development
Saccharomyces cerevisiae
Signal Transduction
Stomach
Chromosomes
Alleles
Gene Expression

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Plass, C., Shibata, H., Kalcheva, I., Mullins, L., Kotelevtseva, N., Mullins, J., ... Chapman, V. M. (1996). Identification of Grf1 on mouse chromosome 9 as an imprinted gene by RLGS-M. Nature genetics, 14(1), 106-109. https://doi.org/10.1038/ng0996-106

Identification of Grf1 on mouse chromosome 9 as an imprinted gene by RLGS-M. / Plass, C.; Shibata, H.; Kalcheva, I.; Mullins, L.; Kotelevtseva, N.; Mullins, J.; Kato, R.; Sasaki, H.; Hirotsune, S.; Okazaki, Y.; Held, W. A.; Hayashizaki, Y.; Chapman, V. M.

In: Nature genetics, Vol. 14, No. 1, 23.09.1996, p. 106-109.

Research output: Contribution to journalArticle

Plass, C, Shibata, H, Kalcheva, I, Mullins, L, Kotelevtseva, N, Mullins, J, Kato, R, Sasaki, H, Hirotsune, S, Okazaki, Y, Held, WA, Hayashizaki, Y & Chapman, VM 1996, 'Identification of Grf1 on mouse chromosome 9 as an imprinted gene by RLGS-M', Nature genetics, vol. 14, no. 1, pp. 106-109. https://doi.org/10.1038/ng0996-106
Plass C, Shibata H, Kalcheva I, Mullins L, Kotelevtseva N, Mullins J et al. Identification of Grf1 on mouse chromosome 9 as an imprinted gene by RLGS-M. Nature genetics. 1996 Sep 23;14(1):106-109. https://doi.org/10.1038/ng0996-106
Plass, C. ; Shibata, H. ; Kalcheva, I. ; Mullins, L. ; Kotelevtseva, N. ; Mullins, J. ; Kato, R. ; Sasaki, H. ; Hirotsune, S. ; Okazaki, Y. ; Held, W. A. ; Hayashizaki, Y. ; Chapman, V. M. / Identification of Grf1 on mouse chromosome 9 as an imprinted gene by RLGS-M. In: Nature genetics. 1996 ; Vol. 14, No. 1. pp. 106-109.
@article{241f0564b8dd4bc8a7c57e23276914d2,
title = "Identification of Grf1 on mouse chromosome 9 as an imprinted gene by RLGS-M",
abstract = "Normal mammalian development requires a diploid combination of both haploid parental genomes. Uniparental disomy for certain segments of specific chromosomes results in aberrant development or prenatal lethality, indicating that the parental genomes have undergone modifications during gametogenesis. These modifications result in parent-of-origin specific expression for some genes, a phenomenon called genomic imprinting. Recent work with DNA methyltransferase deficient mice showed that differential methylation is the probable basis of imprinted character of several genes. Screening for endogenous imprinted loci using restriction landmark genomic scanning with methylation sensitive enzymes (RLGS-M) identified eight imprinted RLGS (Irlgs) candidate loci. Molecular analysis of the genomic region of one of the loci (Irlgs2) resulted in the discovery of the paternally imprinted U2afbp-rs gene within a previously identified imprinted region on mouse chromosome 11 (refs 5, 7). This paper describes the characterisation of a novel imprinted RLGS-M locus, Irlgs3, on mouse chromosome 9 (ref. 6). Within this locus we identified the Grf1 (also called Cdc25(Mm)) gene, which is homologous to the RAS-specific guanine nucleotide exchange factor gene, CDC25, in Saccharomyces cerevisiae. Grf1 is located about 30 kb downstream of the methylation imprinted site, identified by RLGS-M, and shows paternal allele specific expression in mouse brain, stomach and heart. Our results indicate that imprinting may have a role in regulating mitogenic signal transduction pathways during growth and development.",
author = "C. Plass and H. Shibata and I. Kalcheva and L. Mullins and N. Kotelevtseva and J. Mullins and R. Kato and H. Sasaki and S. Hirotsune and Y. Okazaki and Held, {W. A.} and Y. Hayashizaki and Chapman, {V. M.}",
year = "1996",
month = "9",
day = "23",
doi = "10.1038/ng0996-106",
language = "English",
volume = "14",
pages = "106--109",
journal = "Nature Genetics",
issn = "1061-4036",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Identification of Grf1 on mouse chromosome 9 as an imprinted gene by RLGS-M

AU - Plass, C.

AU - Shibata, H.

AU - Kalcheva, I.

AU - Mullins, L.

AU - Kotelevtseva, N.

AU - Mullins, J.

AU - Kato, R.

AU - Sasaki, H.

AU - Hirotsune, S.

AU - Okazaki, Y.

AU - Held, W. A.

AU - Hayashizaki, Y.

AU - Chapman, V. M.

PY - 1996/9/23

Y1 - 1996/9/23

N2 - Normal mammalian development requires a diploid combination of both haploid parental genomes. Uniparental disomy for certain segments of specific chromosomes results in aberrant development or prenatal lethality, indicating that the parental genomes have undergone modifications during gametogenesis. These modifications result in parent-of-origin specific expression for some genes, a phenomenon called genomic imprinting. Recent work with DNA methyltransferase deficient mice showed that differential methylation is the probable basis of imprinted character of several genes. Screening for endogenous imprinted loci using restriction landmark genomic scanning with methylation sensitive enzymes (RLGS-M) identified eight imprinted RLGS (Irlgs) candidate loci. Molecular analysis of the genomic region of one of the loci (Irlgs2) resulted in the discovery of the paternally imprinted U2afbp-rs gene within a previously identified imprinted region on mouse chromosome 11 (refs 5, 7). This paper describes the characterisation of a novel imprinted RLGS-M locus, Irlgs3, on mouse chromosome 9 (ref. 6). Within this locus we identified the Grf1 (also called Cdc25(Mm)) gene, which is homologous to the RAS-specific guanine nucleotide exchange factor gene, CDC25, in Saccharomyces cerevisiae. Grf1 is located about 30 kb downstream of the methylation imprinted site, identified by RLGS-M, and shows paternal allele specific expression in mouse brain, stomach and heart. Our results indicate that imprinting may have a role in regulating mitogenic signal transduction pathways during growth and development.

AB - Normal mammalian development requires a diploid combination of both haploid parental genomes. Uniparental disomy for certain segments of specific chromosomes results in aberrant development or prenatal lethality, indicating that the parental genomes have undergone modifications during gametogenesis. These modifications result in parent-of-origin specific expression for some genes, a phenomenon called genomic imprinting. Recent work with DNA methyltransferase deficient mice showed that differential methylation is the probable basis of imprinted character of several genes. Screening for endogenous imprinted loci using restriction landmark genomic scanning with methylation sensitive enzymes (RLGS-M) identified eight imprinted RLGS (Irlgs) candidate loci. Molecular analysis of the genomic region of one of the loci (Irlgs2) resulted in the discovery of the paternally imprinted U2afbp-rs gene within a previously identified imprinted region on mouse chromosome 11 (refs 5, 7). This paper describes the characterisation of a novel imprinted RLGS-M locus, Irlgs3, on mouse chromosome 9 (ref. 6). Within this locus we identified the Grf1 (also called Cdc25(Mm)) gene, which is homologous to the RAS-specific guanine nucleotide exchange factor gene, CDC25, in Saccharomyces cerevisiae. Grf1 is located about 30 kb downstream of the methylation imprinted site, identified by RLGS-M, and shows paternal allele specific expression in mouse brain, stomach and heart. Our results indicate that imprinting may have a role in regulating mitogenic signal transduction pathways during growth and development.

UR - http://www.scopus.com/inward/record.url?scp=16044371662&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=16044371662&partnerID=8YFLogxK

U2 - 10.1038/ng0996-106

DO - 10.1038/ng0996-106

M3 - Article

C2 - 8782830

AN - SCOPUS:16044371662

VL - 14

SP - 106

EP - 109

JO - Nature Genetics

JF - Nature Genetics

SN - 1061-4036

IS - 1

ER -