Identification of HLA-A24-restricted epitopes with high affinities to Hsp70 using peptide arrays

Mina Okochi; Hiroki Hayashi; Akira Ito; Ryuji Kato; Yasuaki Tamura; Noriyuki Sato; Hiroyuki Honda

doi:10.1263/jbb.105.198

Identification of HLA-A24-restricted epitopes with high affinities to Hsp70 using peptide arrays

Mina Okochi, Hiroki Hayashi, Akira Ito, Ryuji Kato, Yasuaki Tamura, Noriyuki Sato, Hiroyuki Honda

Molecular and Biochemical Systems Engineering

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Heat shock protein 70 (Hsp70) family members are known as facilitators of immune responses by interacting with receptors on antigen-presenting cells leading to Hsp70-peptide uptake and antigen cross priming. Here, identification of human leukocyte antigen (HLA)-A24-restricted epitopes was achieved using peptide arrays for evaluation of their affinities to Hsp70 and HLA-A24 binding prediction tools. Using Hsp70 as the model antigen, the GYPVTNAVI and VFQHGKVEI peptides were identified as antigens. These peptides actually bound to HLA-A24 in the stabilization assay using T2-A*2402 cells, and induced a strong peptide-reactive cytotoxic T lymphocyte response in HLA-A24 transgenic mice after vaccination.

Original language	English
Pages (from-to)	198-203
Number of pages	6
Journal	Journal of Bioscience and Bioengineering
Volume	105
Issue number	3
DOIs	https://doi.org/10.1263/jbb.105.198
Publication status	Published - Mar 2008

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1263/jbb.105.198

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title = "Identification of HLA-A24-restricted epitopes with high affinities to Hsp70 using peptide arrays",

abstract = "Heat shock protein 70 (Hsp70) family members are known as facilitators of immune responses by interacting with receptors on antigen-presenting cells leading to Hsp70-peptide uptake and antigen cross priming. Here, identification of human leukocyte antigen (HLA)-A24-restricted epitopes was achieved using peptide arrays for evaluation of their affinities to Hsp70 and HLA-A24 binding prediction tools. Using Hsp70 as the model antigen, the GYPVTNAVI and VFQHGKVEI peptides were identified as antigens. These peptides actually bound to HLA-A24 in the stabilization assay using T2-A*2402 cells, and induced a strong peptide-reactive cytotoxic T lymphocyte response in HLA-A24 transgenic mice after vaccination.",

author = "Mina Okochi and Hiroki Hayashi and Akira Ito and Ryuji Kato and Yasuaki Tamura and Noriyuki Sato and Hiroyuki Honda",

note = "Funding Information: This study was partly supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science, no. 17206082, a Health and Labor Sciences Research Grant-in-Aid for Research on Advanced Medical Technology from the Ministry of Health, Labour and Welfare. ",

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doi = "10.1263/jbb.105.198",

language = "English",

volume = "105",

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journal = "Journal of Bioscience and Bioengineering",

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publisher = "The Society for Biotechnology, Japan",

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T1 - Identification of HLA-A24-restricted epitopes with high affinities to Hsp70 using peptide arrays

AU - Okochi, Mina

AU - Hayashi, Hiroki

AU - Ito, Akira

AU - Kato, Ryuji

AU - Tamura, Yasuaki

AU - Sato, Noriyuki

AU - Honda, Hiroyuki

N1 - Funding Information: This study was partly supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science, no. 17206082, a Health and Labor Sciences Research Grant-in-Aid for Research on Advanced Medical Technology from the Ministry of Health, Labour and Welfare.

PY - 2008/3

Y1 - 2008/3

N2 - Heat shock protein 70 (Hsp70) family members are known as facilitators of immune responses by interacting with receptors on antigen-presenting cells leading to Hsp70-peptide uptake and antigen cross priming. Here, identification of human leukocyte antigen (HLA)-A24-restricted epitopes was achieved using peptide arrays for evaluation of their affinities to Hsp70 and HLA-A24 binding prediction tools. Using Hsp70 as the model antigen, the GYPVTNAVI and VFQHGKVEI peptides were identified as antigens. These peptides actually bound to HLA-A24 in the stabilization assay using T2-A*2402 cells, and induced a strong peptide-reactive cytotoxic T lymphocyte response in HLA-A24 transgenic mice after vaccination.

AB - Heat shock protein 70 (Hsp70) family members are known as facilitators of immune responses by interacting with receptors on antigen-presenting cells leading to Hsp70-peptide uptake and antigen cross priming. Here, identification of human leukocyte antigen (HLA)-A24-restricted epitopes was achieved using peptide arrays for evaluation of their affinities to Hsp70 and HLA-A24 binding prediction tools. Using Hsp70 as the model antigen, the GYPVTNAVI and VFQHGKVEI peptides were identified as antigens. These peptides actually bound to HLA-A24 in the stabilization assay using T2-A*2402 cells, and induced a strong peptide-reactive cytotoxic T lymphocyte response in HLA-A24 transgenic mice after vaccination.

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ER -

Identification of HLA-A24-restricted epitopes with high affinities to Hsp70 using peptide arrays

Abstract

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