Identification of HLA-A24-restricted epitopes with high affinities to Hsp70 using peptide arrays

Mina Okochi, Hiroki Hayashi, Akira Ito, Ryuji Kato, Yasuaki Tamura, Noriyuki Sato, Hiroyuki Honda

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Heat shock protein 70 (Hsp70) family members are known as facilitators of immune responses by interacting with receptors on antigen-presenting cells leading to Hsp70-peptide uptake and antigen cross priming. Here, identification of human leukocyte antigen (HLA)-A24-restricted epitopes was achieved using peptide arrays for evaluation of their affinities to Hsp70 and HLA-A24 binding prediction tools. Using Hsp70 as the model antigen, the GYPVTNAVI and VFQHGKVEI peptides were identified as antigens. These peptides actually bound to HLA-A24 in the stabilization assay using T2-A*2402 cells, and induced a strong peptide-reactive cytotoxic T lymphocyte response in HLA-A24 transgenic mice after vaccination.

Original languageEnglish
Pages (from-to)198-203
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume105
Issue number3
DOIs
Publication statusPublished - Mar 2008

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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