Identification of hybrid-type carbohydrate chains on the light chain of human monoclonal antibody specific to lung adenocarcinoma

Hirofumi Tachibana, Kiyohiko Seki, Hiroki Murakami

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The carbohydrate chains on the light chains of human monoclonal antibody HB4C5 reactive to human lung adenocarcinoma tissue have been characterized. The HB4C5 antibody consistst of two kinds of light chains (30 kDa and 32 kDa). Each chain has different carbohydrates of varying molecular masses linked to the variable regions. The 30 kDa light chain has been confirmed to be the activity species for antibody binding. The carbohydrates on the light chains were characterized by lectin blot analysis combined with glycosidase treatment. A carbohydrate chain linked to the active 30 kDa light-chain species, and one of a few carbohydrate phenotypes on the 32 kDa light-chain species is characterized as "hybrid type". A sialylated complex-type carbohydrate could also bind to the 32 kDa light-chain species. Nucleotide sequence analysis revealed that one potential N-glycosylation site is located in the complementality determining ergion 1 of the light chain, although no such site was found in the variable region of the heavy chain.

Original languageEnglish
Pages (from-to)257-263
Number of pages7
JournalBBA - Molecular Basis of Disease
Volume1182
Issue number3
DOIs
Publication statusPublished - Oct 20 1993

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Monoclonal Antibodies
Carbohydrates
Light
Adenocarcinoma of lung
Antibodies
Glycoside Hydrolases
Glycosylation
Lectins
Sequence Analysis
Phenotype

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology

Cite this

Identification of hybrid-type carbohydrate chains on the light chain of human monoclonal antibody specific to lung adenocarcinoma. / Tachibana, Hirofumi; Seki, Kiyohiko; Murakami, Hiroki.

In: BBA - Molecular Basis of Disease, Vol. 1182, No. 3, 20.10.1993, p. 257-263.

Research output: Contribution to journalArticle

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