Identification of immune relevant genes using expressed sequence tags (ESTs) in common carp (Cyprinus carpio) gills and intestine

Nevien K. Abdelkhalek, Asuka Komiya, Yoko Kato-Unoki, Tomonori Somamoto, Miki Nakao

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Identification of immune related molecules that function in fish organs is important for better understanding of the host defense mechanisms in fish. Expressed sequence tags (ESTs) represent the expressed portion of a genome, so they have proven to be useful tool for gene identification and confirmation of gene predictions. In the present study, a transcriptome analysis of carp gill and intestine EST libraries have been done as an attempt to identify the immune relevant genes expressed in those organs. A total of 2148 EST clones were generated from the two libraries: 1099 clones from gill library in which 632 clones were matched with functional proteins and 1049 clones from intestine library in which 559 clones were matched with functional proteins. The results of gill EST library has more frequency of innate immune-molecules containing MHC class I and MHC class II, which showed 18.3% of the immune molecules than those in intestine library which represents 10.8% only. The gill library also showed higher frequency of the cytokines and chemokine molecules and/or their receptors compared to the intestine library.

Original languageEnglish
Pages (from-to)109-116
Number of pages8
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume59
Issue number1
Publication statusPublished - Feb 1 2014

Fingerprint

Carps
Expressed Sequence Tags
expressed sequence tags
Cyprinus carpio
Intestines
gills
intestines
Clone Cells
Genes
clones
genes
Fishes
Gene Expression Profiling
Chemokines
Libraries
Proteins
Genome
Cytokines
chemokines
transcriptomics

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Identification of immune relevant genes using expressed sequence tags (ESTs) in common carp (Cyprinus carpio) gills and intestine. / Abdelkhalek, Nevien K.; Komiya, Asuka; Kato-Unoki, Yoko; Somamoto, Tomonori; Nakao, Miki.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 59, No. 1, 01.02.2014, p. 109-116.

Research output: Contribution to journalArticle

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