@article{71bcbd74fc1b44189fe74bfd779476af,
title = "Identification of intracellular squalene in living algae, Aurantiochytrium mangrovei with hyper-spectral coherent anti-Stokes Raman microscopy using a sub-nanosecond supercontinuum laser source",
abstract = "We applied hyper-spectral coherent anti-Stokes Raman scattering imaging to intracellular lipid identification in living microalgae, Aurantiochytrium mangrovei 18W-13a. Two different lipids, squalene and triacylglycerol, were found inside living cells with clear vibrational contrast. Based on the endogenous lipid band as a result of the cis CC stretch vibrational mode, squalene and triacylglycerol were clearly distinguished in different intracellular areas. In particular, squalene was detected solely in vacuoles as lipid particles, which was also supported by electron microscopy.",
author = "Kei Ishitsuka and Masahiro Koide and Masaki Yoshida and Hiroki Segawa and Philippe Leproux and Vincent Couderc and Watanabe, {Makoto M.} and Hideaki Kano",
note = "Funding Information: This study was financially supported by Photographic Research Fund of Konica Minolta Imaging Science Foundation to HK and JSPS KAKENHI (grant number 23770234) and Mayekawa Houonkai Foundation to MY. The authors thank the LEUKOS company for technical support with the dual-output supercontinuum light source. The authors gratefully acknowledge Juichiro Ukon, UKON CRAFT SCIENCE, Ltd. for assisting with a fruitful collaboration between Japanese and French labs. Publisher Copyright: Copyright {\textcopyright} 2016 John Wiley & Sons, Ltd.",
year = "2017",
month = jan,
day = "1",
doi = "10.1002/jrs.4979",
language = "English",
volume = "48",
pages = "8--15",
journal = "Journal of Raman Spectroscopy",
issn = "0377-0486",
publisher = "John Wiley and Sons Ltd",
number = "1",
}