Identification of intracellular squalene in living algae, Aurantiochytrium mangrovei with hyper-spectral coherent anti-Stokes Raman microscopy using a sub-nanosecond supercontinuum laser source

Kei Ishitsuka; Masahiro Koide; Masaki Yoshida; Hiroki Segawa; Philippe Leproux; Vincent Couderc; Makoto M. Watanabe; Hideaki Kano

doi:10.1002/jrs.4979

Identification of intracellular squalene in living algae, Aurantiochytrium mangrovei with hyper-spectral coherent anti-Stokes Raman microscopy using a sub-nanosecond supercontinuum laser source

Kei Ishitsuka, Masahiro Koide, Masaki Yoshida, Hiroki Segawa, Philippe Leproux, Vincent Couderc, Makoto M. Watanabe, Hideaki Kano

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

We applied hyper-spectral coherent anti-Stokes Raman scattering imaging to intracellular lipid identification in living microalgae, Aurantiochytrium mangrovei 18W-13a. Two different lipids, squalene and triacylglycerol, were found inside living cells with clear vibrational contrast. Based on the endogenous lipid band as a result of the cis CC stretch vibrational mode, squalene and triacylglycerol were clearly distinguished in different intracellular areas. In particular, squalene was detected solely in vacuoles as lipid particles, which was also supported by electron microscopy.

Original language	English
Pages (from-to)	8-15
Number of pages	8
Journal	Journal of Raman Spectroscopy
Volume	48
Issue number	1
DOIs	https://doi.org/10.1002/jrs.4979
Publication status	Published - Jan 1 2017
Externally published	Yes

All Science Journal Classification (ASJC) codes

Materials Science(all)
Spectroscopy

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10.1002/jrs.4979

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Ishitsuka, K., Koide, M., Yoshida, M., Segawa, H., Leproux, P., Couderc, V., Watanabe, M. M., & Kano, H. (2017). Identification of intracellular squalene in living algae, Aurantiochytrium mangrovei with hyper-spectral coherent anti-Stokes Raman microscopy using a sub-nanosecond supercontinuum laser source. Journal of Raman Spectroscopy, 48(1), 8-15. https://doi.org/10.1002/jrs.4979

Identification of intracellular squalene in living algae, Aurantiochytrium mangrovei with hyper-spectral coherent anti-Stokes Raman microscopy using a sub-nanosecond supercontinuum laser source. / Ishitsuka, Kei; Koide, Masahiro; Yoshida, Masaki; Segawa, Hiroki; Leproux, Philippe; Couderc, Vincent; Watanabe, Makoto M.; Kano, Hideaki.

In: Journal of Raman Spectroscopy, Vol. 48, No. 1, 01.01.2017, p. 8-15.

Research output: Contribution to journal › Article › peer-review

Ishitsuka, K, Koide, M, Yoshida, M, Segawa, H, Leproux, P, Couderc, V, Watanabe, MM & Kano, H 2017, 'Identification of intracellular squalene in living algae, Aurantiochytrium mangrovei with hyper-spectral coherent anti-Stokes Raman microscopy using a sub-nanosecond supercontinuum laser source', Journal of Raman Spectroscopy, vol. 48, no. 1, pp. 8-15. https://doi.org/10.1002/jrs.4979

Ishitsuka, Kei ; Koide, Masahiro ; Yoshida, Masaki ; Segawa, Hiroki ; Leproux, Philippe ; Couderc, Vincent ; Watanabe, Makoto M. ; Kano, Hideaki. / Identification of intracellular squalene in living algae, Aurantiochytrium mangrovei with hyper-spectral coherent anti-Stokes Raman microscopy using a sub-nanosecond supercontinuum laser source. In: Journal of Raman Spectroscopy. 2017 ; Vol. 48, No. 1. pp. 8-15.

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abstract = "We applied hyper-spectral coherent anti-Stokes Raman scattering imaging to intracellular lipid identification in living microalgae, Aurantiochytrium mangrovei 18W-13a. Two different lipids, squalene and triacylglycerol, were found inside living cells with clear vibrational contrast. Based on the endogenous lipid band as a result of the cis CC stretch vibrational mode, squalene and triacylglycerol were clearly distinguished in different intracellular areas. In particular, squalene was detected solely in vacuoles as lipid particles, which was also supported by electron microscopy.",

author = "Kei Ishitsuka and Masahiro Koide and Masaki Yoshida and Hiroki Segawa and Philippe Leproux and Vincent Couderc and Watanabe, {Makoto M.} and Hideaki Kano",

note = "Funding Information: This study was financially supported by Photographic Research Fund of Konica Minolta Imaging Science Foundation to HK and JSPS KAKENHI (grant number 23770234) and Mayekawa Houonkai Foundation to MY. The authors thank the LEUKOS company for technical support with the dual-output supercontinuum light source. The authors gratefully acknowledge Juichiro Ukon, UKON CRAFT SCIENCE, Ltd. for assisting with a fruitful collaboration between Japanese and French labs.",

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AB - We applied hyper-spectral coherent anti-Stokes Raman scattering imaging to intracellular lipid identification in living microalgae, Aurantiochytrium mangrovei 18W-13a. Two different lipids, squalene and triacylglycerol, were found inside living cells with clear vibrational contrast. Based on the endogenous lipid band as a result of the cis CC stretch vibrational mode, squalene and triacylglycerol were clearly distinguished in different intracellular areas. In particular, squalene was detected solely in vacuoles as lipid particles, which was also supported by electron microscopy.

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