Identification of leptospiral 3-hydroxyacyl-CoA dehydrogenase released in the urine of infected hamsters

Takaya Segawa, Kazuko H. Nomura, Sharon Yvette Angelina M. Villanueva, Mitsumasa Saito, Kazuya Nomura, Nina G. Gloriani, Shin Ichi Yoshida

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Leptospirosis is a global zoonosis caused by pathogenic Leptospira. The non-specific clinical signs and symptoms of leptospirosis lead to its misdiagnosis. To date, there is still no reliable rapid test kit that can accurately diagnose leptospirosis at bedside or in field. In this research, with the ultimate goal of formulating a rapid and accurate diagnostic tool for leptospirosis, we aimed to identify leptospiral proteins excreted in urine of infected hamsters, which are thought to mimic Weil's disease. Results: Hamsters were subcutaneously infected with leptospires, and the general attributes of urine as well as the proteins excreted in it were examined. Some leptospiral proteins were found to be excreted in the urine from the early phase of infection. The most important finding of this study was the detection of the lipid-metabolizing enzyme, 3-hydroxyacyl-CoA dehydrogenase (HADH), before the onset of illness, when leptospires were not yet detected in the urine of infected hamsters. Conclusions: This is the first report on the detection of leptospiral HADH in the host urine, which may be a possible candidate leptospiral antigen that can be used in the early diagnosis of human and animal leptospirosis.

Original languageEnglish
Article number132
JournalBMC Microbiology
Volume14
Issue number1
DOIs
Publication statusPublished - May 21 2014

Fingerprint

3-Hydroxyacyl-CoA Dehydrogenase
Leptospirosis
Cricetinae
Urine
Leptospira
Proteins
Zoonoses
Diagnostic Errors
Signs and Symptoms
Early Diagnosis
Lipids
Antigens
Enzymes
Infection
Research

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

Segawa, T., Nomura, K. H., Villanueva, S. Y. A. M., Saito, M., Nomura, K., Gloriani, N. G., & Yoshida, S. I. (2014). Identification of leptospiral 3-hydroxyacyl-CoA dehydrogenase released in the urine of infected hamsters. BMC Microbiology, 14(1), [132]. https://doi.org/10.1186/1471-2180-14-132

Identification of leptospiral 3-hydroxyacyl-CoA dehydrogenase released in the urine of infected hamsters. / Segawa, Takaya; Nomura, Kazuko H.; Villanueva, Sharon Yvette Angelina M.; Saito, Mitsumasa; Nomura, Kazuya; Gloriani, Nina G.; Yoshida, Shin Ichi.

In: BMC Microbiology, Vol. 14, No. 1, 132, 21.05.2014.

Research output: Contribution to journalArticle

Segawa, T, Nomura, KH, Villanueva, SYAM, Saito, M, Nomura, K, Gloriani, NG & Yoshida, SI 2014, 'Identification of leptospiral 3-hydroxyacyl-CoA dehydrogenase released in the urine of infected hamsters', BMC Microbiology, vol. 14, no. 1, 132. https://doi.org/10.1186/1471-2180-14-132
Segawa, Takaya ; Nomura, Kazuko H. ; Villanueva, Sharon Yvette Angelina M. ; Saito, Mitsumasa ; Nomura, Kazuya ; Gloriani, Nina G. ; Yoshida, Shin Ichi. / Identification of leptospiral 3-hydroxyacyl-CoA dehydrogenase released in the urine of infected hamsters. In: BMC Microbiology. 2014 ; Vol. 14, No. 1.
@article{30e0b190c26f43309eaa0893f2c67367,
title = "Identification of leptospiral 3-hydroxyacyl-CoA dehydrogenase released in the urine of infected hamsters",
abstract = "Background: Leptospirosis is a global zoonosis caused by pathogenic Leptospira. The non-specific clinical signs and symptoms of leptospirosis lead to its misdiagnosis. To date, there is still no reliable rapid test kit that can accurately diagnose leptospirosis at bedside or in field. In this research, with the ultimate goal of formulating a rapid and accurate diagnostic tool for leptospirosis, we aimed to identify leptospiral proteins excreted in urine of infected hamsters, which are thought to mimic Weil's disease. Results: Hamsters were subcutaneously infected with leptospires, and the general attributes of urine as well as the proteins excreted in it were examined. Some leptospiral proteins were found to be excreted in the urine from the early phase of infection. The most important finding of this study was the detection of the lipid-metabolizing enzyme, 3-hydroxyacyl-CoA dehydrogenase (HADH), before the onset of illness, when leptospires were not yet detected in the urine of infected hamsters. Conclusions: This is the first report on the detection of leptospiral HADH in the host urine, which may be a possible candidate leptospiral antigen that can be used in the early diagnosis of human and animal leptospirosis.",
author = "Takaya Segawa and Nomura, {Kazuko H.} and Villanueva, {Sharon Yvette Angelina M.} and Mitsumasa Saito and Kazuya Nomura and Gloriani, {Nina G.} and Yoshida, {Shin Ichi}",
year = "2014",
month = "5",
day = "21",
doi = "10.1186/1471-2180-14-132",
language = "English",
volume = "14",
journal = "BMC Microbiology",
issn = "1471-2180",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Identification of leptospiral 3-hydroxyacyl-CoA dehydrogenase released in the urine of infected hamsters

AU - Segawa, Takaya

AU - Nomura, Kazuko H.

AU - Villanueva, Sharon Yvette Angelina M.

AU - Saito, Mitsumasa

AU - Nomura, Kazuya

AU - Gloriani, Nina G.

AU - Yoshida, Shin Ichi

PY - 2014/5/21

Y1 - 2014/5/21

N2 - Background: Leptospirosis is a global zoonosis caused by pathogenic Leptospira. The non-specific clinical signs and symptoms of leptospirosis lead to its misdiagnosis. To date, there is still no reliable rapid test kit that can accurately diagnose leptospirosis at bedside or in field. In this research, with the ultimate goal of formulating a rapid and accurate diagnostic tool for leptospirosis, we aimed to identify leptospiral proteins excreted in urine of infected hamsters, which are thought to mimic Weil's disease. Results: Hamsters were subcutaneously infected with leptospires, and the general attributes of urine as well as the proteins excreted in it were examined. Some leptospiral proteins were found to be excreted in the urine from the early phase of infection. The most important finding of this study was the detection of the lipid-metabolizing enzyme, 3-hydroxyacyl-CoA dehydrogenase (HADH), before the onset of illness, when leptospires were not yet detected in the urine of infected hamsters. Conclusions: This is the first report on the detection of leptospiral HADH in the host urine, which may be a possible candidate leptospiral antigen that can be used in the early diagnosis of human and animal leptospirosis.

AB - Background: Leptospirosis is a global zoonosis caused by pathogenic Leptospira. The non-specific clinical signs and symptoms of leptospirosis lead to its misdiagnosis. To date, there is still no reliable rapid test kit that can accurately diagnose leptospirosis at bedside or in field. In this research, with the ultimate goal of formulating a rapid and accurate diagnostic tool for leptospirosis, we aimed to identify leptospiral proteins excreted in urine of infected hamsters, which are thought to mimic Weil's disease. Results: Hamsters were subcutaneously infected with leptospires, and the general attributes of urine as well as the proteins excreted in it were examined. Some leptospiral proteins were found to be excreted in the urine from the early phase of infection. The most important finding of this study was the detection of the lipid-metabolizing enzyme, 3-hydroxyacyl-CoA dehydrogenase (HADH), before the onset of illness, when leptospires were not yet detected in the urine of infected hamsters. Conclusions: This is the first report on the detection of leptospiral HADH in the host urine, which may be a possible candidate leptospiral antigen that can be used in the early diagnosis of human and animal leptospirosis.

UR - http://www.scopus.com/inward/record.url?scp=84901689328&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901689328&partnerID=8YFLogxK

U2 - 10.1186/1471-2180-14-132

DO - 10.1186/1471-2180-14-132

M3 - Article

C2 - 24884439

AN - SCOPUS:84901689328

VL - 14

JO - BMC Microbiology

JF - BMC Microbiology

SN - 1471-2180

IS - 1

M1 - 132

ER -