TY - JOUR
T1 - Identification of leptospiral 3-hydroxyacyl-CoA dehydrogenase released in the urine of infected hamsters
AU - Segawa, Takaya
AU - Nomura, Kazuko H.
AU - Villanueva, Sharon Yvette Angelina M.
AU - Saito, Mitsumasa
AU - Nomura, Kazuya
AU - Gloriani, Nina G.
AU - Yoshida, Shin Ichi
N1 - Funding Information:
This study was supported by a grant of the Science and Technology Research Partnership for Sustainable Development (SATREPS) program from Japan Science and Technology Agency (JST) and Japan International Cooperation Agency (JICA). We thank Dr. H. Sumimoto and colleagues of the Research Support Center, Graduate School of Medical Sciences, Kyushu University for their technical support and advice. We also thank Sayaka Akiyoshi, Takayoshi Yamaguchi, Hideko Kameyama, and Naomi Hidaka for their technical cooperation.
PY - 2014/5/21
Y1 - 2014/5/21
N2 - Background: Leptospirosis is a global zoonosis caused by pathogenic Leptospira. The non-specific clinical signs and symptoms of leptospirosis lead to its misdiagnosis. To date, there is still no reliable rapid test kit that can accurately diagnose leptospirosis at bedside or in field. In this research, with the ultimate goal of formulating a rapid and accurate diagnostic tool for leptospirosis, we aimed to identify leptospiral proteins excreted in urine of infected hamsters, which are thought to mimic Weil's disease. Results: Hamsters were subcutaneously infected with leptospires, and the general attributes of urine as well as the proteins excreted in it were examined. Some leptospiral proteins were found to be excreted in the urine from the early phase of infection. The most important finding of this study was the detection of the lipid-metabolizing enzyme, 3-hydroxyacyl-CoA dehydrogenase (HADH), before the onset of illness, when leptospires were not yet detected in the urine of infected hamsters. Conclusions: This is the first report on the detection of leptospiral HADH in the host urine, which may be a possible candidate leptospiral antigen that can be used in the early diagnosis of human and animal leptospirosis.
AB - Background: Leptospirosis is a global zoonosis caused by pathogenic Leptospira. The non-specific clinical signs and symptoms of leptospirosis lead to its misdiagnosis. To date, there is still no reliable rapid test kit that can accurately diagnose leptospirosis at bedside or in field. In this research, with the ultimate goal of formulating a rapid and accurate diagnostic tool for leptospirosis, we aimed to identify leptospiral proteins excreted in urine of infected hamsters, which are thought to mimic Weil's disease. Results: Hamsters were subcutaneously infected with leptospires, and the general attributes of urine as well as the proteins excreted in it were examined. Some leptospiral proteins were found to be excreted in the urine from the early phase of infection. The most important finding of this study was the detection of the lipid-metabolizing enzyme, 3-hydroxyacyl-CoA dehydrogenase (HADH), before the onset of illness, when leptospires were not yet detected in the urine of infected hamsters. Conclusions: This is the first report on the detection of leptospiral HADH in the host urine, which may be a possible candidate leptospiral antigen that can be used in the early diagnosis of human and animal leptospirosis.
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U2 - 10.1186/1471-2180-14-132
DO - 10.1186/1471-2180-14-132
M3 - Article
C2 - 24884439
AN - SCOPUS:84901689328
SN - 1471-2180
VL - 14
JO - BMC Microbiology
JF - BMC Microbiology
IS - 1
M1 - 132
ER -
