TY - JOUR
T1 - Identification of metabolites associated with onset of CAD in diabetic patients using ce-ms analysis
T2 - A pilot study
AU - Omori, Kazuo
AU - Katakami, Naoto
AU - Yamamoto, Yuichi
AU - Ninomiya, Hiroyo
AU - Takahara, Mitsuyoshi
AU - Matsuoka, Taka Aki
AU - Bamba, Takeshi
AU - Fukusaki, Eiichiro
AU - Shimomura, Iichiro
N1 - Funding Information:
This research was supported by AMED-CREST, Japan Agency for Medical Research and Development (AMED) (Grant Number JP18gm0710005).
Publisher Copyright:
© 2019 Japan Atherosclerosis Society.
PY - 2019
Y1 - 2019
N2 - Aim: Coronary artery disease (CAD) is the result of a complex metabolic disorder caused by various environmental and genetic factors. Metabolomics is a potential tool for identifying biomarkers for better risk classification and for understanding the pathophysiological mechanisms of CAD. With this background, we performed a pilot study to identify metabolites associated with the future onset of CAD in patients with type 2 diabetes. Methods: Sixteen subjects who suffered from CAD event during the observation period and 39 non-CAD subjects who were matched to the CAD subjects for Framingham Coronary Heart Disease Risk Score, diabetes duration, and HbA1c were selected. Capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) was used to perform non-targeted metabolome analysis of serum samples collected in 2005. Results: A total of 104 metabolites were identified. Unsupervised principal component analysis (PCA) did not to reveal two distinct clusters of individuals. However, a significant association with CAD was found for 7 metabolites (pelargonic acid, glucosamine: galactosamine, thymine, 3-hydroxybutyric acid, creatine, 2-aminoiso-butyric acid, hypoxanthine) and the levels of all these metabolites were significantly lower in the CAD group compared with the non-CAD group. Conclusions: We identified 7 metabolites related to long-term future onset of CAD in Japanese patients with diabetes. Further studies with large sample size would be necessary to confirm our findings, and future studies using in vivo or in vitro models would be necessary to elucidate whether direct relationships exist between the detected metabolites and CAD pathophysiology.
AB - Aim: Coronary artery disease (CAD) is the result of a complex metabolic disorder caused by various environmental and genetic factors. Metabolomics is a potential tool for identifying biomarkers for better risk classification and for understanding the pathophysiological mechanisms of CAD. With this background, we performed a pilot study to identify metabolites associated with the future onset of CAD in patients with type 2 diabetes. Methods: Sixteen subjects who suffered from CAD event during the observation period and 39 non-CAD subjects who were matched to the CAD subjects for Framingham Coronary Heart Disease Risk Score, diabetes duration, and HbA1c were selected. Capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) was used to perform non-targeted metabolome analysis of serum samples collected in 2005. Results: A total of 104 metabolites were identified. Unsupervised principal component analysis (PCA) did not to reveal two distinct clusters of individuals. However, a significant association with CAD was found for 7 metabolites (pelargonic acid, glucosamine: galactosamine, thymine, 3-hydroxybutyric acid, creatine, 2-aminoiso-butyric acid, hypoxanthine) and the levels of all these metabolites were significantly lower in the CAD group compared with the non-CAD group. Conclusions: We identified 7 metabolites related to long-term future onset of CAD in Japanese patients with diabetes. Further studies with large sample size would be necessary to confirm our findings, and future studies using in vivo or in vitro models would be necessary to elucidate whether direct relationships exist between the detected metabolites and CAD pathophysiology.
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U2 - 10.5551/JAT.42945
DO - 10.5551/JAT.42945
M3 - Article
C2 - 30068816
AN - SCOPUS:85062428507
SN - 1340-3478
VL - 26
SP - 233
EP - 245
JO - Journal of Atherosclerosis and Thrombosis
JF - Journal of Atherosclerosis and Thrombosis
IS - 3
ER -
