Identification of metabolites associated with onset of CAD in diabetic patients using ce-ms analysis: A pilot study

Kazuo Omori, Naoto Katakami, Yuichi Yamamoto, Hiroyo Ninomiya, Mitsuyoshi Takahara, Taka Aki Matsuoka, Takeshi Bamba, Eiichiro Fukusaki, Iichiro Shimomura

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Aim: Coronary artery disease (CAD) is the result of a complex metabolic disorder caused by various environmental and genetic factors. Metabolomics is a potential tool for identifying biomarkers for better risk classification and for understanding the pathophysiological mechanisms of CAD. With this background, we performed a pilot study to identify metabolites associated with the future onset of CAD in patients with type 2 diabetes. Methods: Sixteen subjects who suffered from CAD event during the observation period and 39 non-CAD subjects who were matched to the CAD subjects for Framingham Coronary Heart Disease Risk Score, diabetes duration, and HbA1c were selected. Capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) was used to perform non-targeted metabolome analysis of serum samples collected in 2005. Results: A total of 104 metabolites were identified. Unsupervised principal component analysis (PCA) did not to reveal two distinct clusters of individuals. However, a significant association with CAD was found for 7 metabolites (pelargonic acid, glucosamine: galactosamine, thymine, 3-hydroxybutyric acid, creatine, 2-aminoiso-butyric acid, hypoxanthine) and the levels of all these metabolites were significantly lower in the CAD group compared with the non-CAD group. Conclusions: We identified 7 metabolites related to long-term future onset of CAD in Japanese patients with diabetes. Further studies with large sample size would be necessary to confirm our findings, and future studies using in vivo or in vitro models would be necessary to elucidate whether direct relationships exist between the detected metabolites and CAD pathophysiology.

Original languageEnglish
Pages (from-to)233-245
Number of pages13
JournalJournal of atherosclerosis and thrombosis
Volume26
Issue number3
DOIs
Publication statusPublished - Jan 1 2019

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Metabolites
Coronary Artery Disease
Medical problems
Arteries
Galactosamine
Hypoxanthine
Thymine
Metabolomics
Butyric Acid
3-Hydroxybutyric Acid
Metabolome
Creatine
Glucosamine
Capillary Electrophoresis
Principal Component Analysis
Capillary electrophoresis
Sample Size
Type 2 Diabetes Mellitus
Coronary Disease
Mass Spectrometry

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Cardiology and Cardiovascular Medicine
  • Biochemistry, medical

Cite this

Omori, K., Katakami, N., Yamamoto, Y., Ninomiya, H., Takahara, M., Matsuoka, T. A., ... Shimomura, I. (2019). Identification of metabolites associated with onset of CAD in diabetic patients using ce-ms analysis: A pilot study. Journal of atherosclerosis and thrombosis, 26(3), 233-245. https://doi.org/10.5551/JAT.42945

Identification of metabolites associated with onset of CAD in diabetic patients using ce-ms analysis : A pilot study. / Omori, Kazuo; Katakami, Naoto; Yamamoto, Yuichi; Ninomiya, Hiroyo; Takahara, Mitsuyoshi; Matsuoka, Taka Aki; Bamba, Takeshi; Fukusaki, Eiichiro; Shimomura, Iichiro.

In: Journal of atherosclerosis and thrombosis, Vol. 26, No. 3, 01.01.2019, p. 233-245.

Research output: Contribution to journalArticle

Omori, K, Katakami, N, Yamamoto, Y, Ninomiya, H, Takahara, M, Matsuoka, TA, Bamba, T, Fukusaki, E & Shimomura, I 2019, 'Identification of metabolites associated with onset of CAD in diabetic patients using ce-ms analysis: A pilot study', Journal of atherosclerosis and thrombosis, vol. 26, no. 3, pp. 233-245. https://doi.org/10.5551/JAT.42945
Omori, Kazuo ; Katakami, Naoto ; Yamamoto, Yuichi ; Ninomiya, Hiroyo ; Takahara, Mitsuyoshi ; Matsuoka, Taka Aki ; Bamba, Takeshi ; Fukusaki, Eiichiro ; Shimomura, Iichiro. / Identification of metabolites associated with onset of CAD in diabetic patients using ce-ms analysis : A pilot study. In: Journal of atherosclerosis and thrombosis. 2019 ; Vol. 26, No. 3. pp. 233-245.
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AU - Omori, Kazuo

AU - Katakami, Naoto

AU - Yamamoto, Yuichi

AU - Ninomiya, Hiroyo

AU - Takahara, Mitsuyoshi

AU - Matsuoka, Taka Aki

AU - Bamba, Takeshi

AU - Fukusaki, Eiichiro

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N2 - Aim: Coronary artery disease (CAD) is the result of a complex metabolic disorder caused by various environmental and genetic factors. Metabolomics is a potential tool for identifying biomarkers for better risk classification and for understanding the pathophysiological mechanisms of CAD. With this background, we performed a pilot study to identify metabolites associated with the future onset of CAD in patients with type 2 diabetes. Methods: Sixteen subjects who suffered from CAD event during the observation period and 39 non-CAD subjects who were matched to the CAD subjects for Framingham Coronary Heart Disease Risk Score, diabetes duration, and HbA1c were selected. Capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) was used to perform non-targeted metabolome analysis of serum samples collected in 2005. Results: A total of 104 metabolites were identified. Unsupervised principal component analysis (PCA) did not to reveal two distinct clusters of individuals. However, a significant association with CAD was found for 7 metabolites (pelargonic acid, glucosamine: galactosamine, thymine, 3-hydroxybutyric acid, creatine, 2-aminoiso-butyric acid, hypoxanthine) and the levels of all these metabolites were significantly lower in the CAD group compared with the non-CAD group. Conclusions: We identified 7 metabolites related to long-term future onset of CAD in Japanese patients with diabetes. Further studies with large sample size would be necessary to confirm our findings, and future studies using in vivo or in vitro models would be necessary to elucidate whether direct relationships exist between the detected metabolites and CAD pathophysiology.

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