Identification of miR-305, a microRNA that promotes aging, and its target mRNAs in Drosophila

Makiko Ueda, Tetsuya Sato, Yasuyuki Ohkawa, Yoshihiro H. Inoue

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are involved in the regulation of important biological processes. Here, we describe a novel Drosophila miRNAs involved in aging. We selected eight Drosophila miRNAs, displaying high homology with seed sequences of aging-related miRNAs characterized in other species, and investigated whether the over-expression of these miRNAs affected aging in Drosophila adult flies. The lifespan of adults over-expressing miR-305, a miRNA showing high homology with miR-239 in C. elegans, was significantly shorter. Conversely, a reduction in miR-305 expression led to a longer lifespan than that in control flies. miR-305 over-expression accelerated the impairment of locomotor activity and promoted the age-dependent accumulation of poly-ubiquitinated protein aggregates in the muscle, as flies aged. Thus, we show that the ectopic expression of miR-305 has a deleterious effect on aging in Drosophila. To identify the targets of miR-305, we performed RNA-Seq. We discovered several mRNAs encoding antimicrobial peptides and insulin-like peptides, whose expression changed in adults upon miR-305 over-expression. We further confirmed, by qRT-PCR, that miR-305 over-expression significantly decreases the mRNA levels of four antimicrobial peptides. As these mRNAs contain multiple sequences matching the seed sequence of miR-305, we speculate that a reduction in target mRNA levels, caused by ectopic miRNA expression, promotes aging.

Original languageEnglish
Pages (from-to)80-93
Number of pages14
JournalGenes to Cells
Volume23
Issue number2
DOIs
Publication statusPublished - Feb 1 2018

Fingerprint

MicroRNAs
Drosophila
Messenger RNA
Diptera
Peptides
Seeds
Ubiquitinated Proteins
Biological Phenomena
Locomotion
RNA
Insulin
Muscles
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

Identification of miR-305, a microRNA that promotes aging, and its target mRNAs in Drosophila. / Ueda, Makiko; Sato, Tetsuya; Ohkawa, Yasuyuki; Inoue, Yoshihiro H.

In: Genes to Cells, Vol. 23, No. 2, 01.02.2018, p. 80-93.

Research output: Contribution to journalArticle

Ueda, Makiko ; Sato, Tetsuya ; Ohkawa, Yasuyuki ; Inoue, Yoshihiro H. / Identification of miR-305, a microRNA that promotes aging, and its target mRNAs in Drosophila. In: Genes to Cells. 2018 ; Vol. 23, No. 2. pp. 80-93.
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