Identification of new secreted proteins and secretion of heterologous amylase by C. glutamicum

Nobuaki Suzuki, Keiro Watanabe, Naoko Okibe, Yoshiki Tsuchida, Masayuki Inui, Hideaki Yukawa

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In this study, secreted Corynebacterium glutamicum proteins were investigated by two-dimensional gel electrophoresis. Around 100 spots observed in the pH range 4.5-5.5 had molecular masses that varied from 10 to 50 kDa. Upon N-terminal amino acid sequence analysis by Edman degradation, two of them were hits to two hypothetical proteins encoded by cgR-1176 and cgR-2070 on C. glutamicum R genome, respectively. Active-form α-amylase derived from Geobacillus stearothermophilus was successfully secreted by using the predicted cgR-1176 and cgR-2070 signal sequences, indicating that these hypothetical proteins were secreted proteins. Analysis using a disruption mutant of the twin-arginine translocation (Tat) export pathway machinery of C. glutamicum suggested that one is Tat pathway dependent secretion while the other is independent of the pathway. Our results demonstrate that C. glutamicum can secrete exoproteins by using its own signal sequences, indicating its potential as a host for protein productions.

Original languageEnglish
Pages (from-to)491-500
Number of pages10
JournalApplied Microbiology and Biotechnology
Volume82
Issue number3
DOIs
Publication statusPublished - Mar 1 2009
Externally publishedYes

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Corynebacterium glutamicum
Amylases
Protein Sorting Signals
Proteins
Arginine
Geobacillus stearothermophilus
Secretory Pathway
Protein Sequence Analysis
Electrophoresis, Gel, Two-Dimensional
Genome

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Identification of new secreted proteins and secretion of heterologous amylase by C. glutamicum. / Suzuki, Nobuaki; Watanabe, Keiro; Okibe, Naoko; Tsuchida, Yoshiki; Inui, Masayuki; Yukawa, Hideaki.

In: Applied Microbiology and Biotechnology, Vol. 82, No. 3, 01.03.2009, p. 491-500.

Research output: Contribution to journalArticle

Suzuki, Nobuaki ; Watanabe, Keiro ; Okibe, Naoko ; Tsuchida, Yoshiki ; Inui, Masayuki ; Yukawa, Hideaki. / Identification of new secreted proteins and secretion of heterologous amylase by C. glutamicum. In: Applied Microbiology and Biotechnology. 2009 ; Vol. 82, No. 3. pp. 491-500.
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