Identification of novel genes with prognostic value in childhood leukemia using cDNA microarray and quantitative RT-PCR

Hiroyoshi Hattori, Akinobu Matsukaki, Aiko Suminoe, Yuhki Koga, Kosuke Tashiro, Toshiro Hara

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The aim of this study was to identify genes distinctively expressed or suppressed in childhood leukemia with different prognoses, using cDNA microarray and quantitative reverse transcription-polymerase chain reaction (RT-PCR). The expression levels of the selected genes by cDNA microarray were quantified in primary leukemic blasts from 44 patients (acute lymphoblastic leukemia, 28; acute myelogenous leukemia (AML), 13; transient myeloproliferative disorder, 3). The expression levels of CDKN2C, CRADD, and IGFBP-2 genes were significantly associated with the event-free survival of the patients in AML. The present results suggest that a combination of cDNA microarray and quantitative RT-PCR may be useful to identify novel genes with prognostic value in childhood AML.

Original languageEnglish
Pages (from-to)115-127
Number of pages13
JournalPediatric Hematology and Oncology
Volume23
Issue number2
DOIs
Publication statusPublished - Mar 2006

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health
  • Hematology
  • Oncology

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