Identification of oxidized methionine sites in erythrocyte membrane protein by liquid chromatography/electrospray ionization mass spectrometry peptide mapping

Chunyan Li, Shinya Takazaki, Xiuri Jin, Dongchon Kang, Yoshito Abe, Naotaka Hamasaki

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In this study, we used peptide mapping combined with liquid chromatography/electrospray ionization mass spectrometry (LC/ESI MS) to examine the methionine oxidation of band 3 of erythrocyte membrane protein. Initially, we identified the methionine sites oxidized by chloramine T (N-chloro-p- toluenesulfoamide), a hydrophilic reagent. There were three oxidized methionines (Met 559, Met 741, and Met 909) in band 3, and these methionines were located in a hydrophilic region determined by previous topological studies of band 3. In addition, we found that C 12E 8, a polyoxyethylene detergent, leads to the oxidation of methionines in a transmembrane segment in band 3, and this oxidation occurs in a C12E8 preincubation time-dependent manner. In a previous study, it was found that peroxides accumulate in a polyoxyethylene detergent. Thus, our method enabled the direct and quantitative detection of protein damage due to detergent peroxides. Furthermore, we examined methionine oxidation in the presence of 4,4′-dinitrostilbene-2,2′- disulfonic acid (DNDS) or diethyl pyrocarbonate (DEPC), which induced either an outward or an inward conformation in band 3, respectively. Our results indicated that the location of Met 741 was associated with the band 3 conformation induced by band 3-mediated anion transport. In conclusion, we found that methionine oxidation can be applied to examine membrane protein structures as follows: (1) for topological studies of membrane proteins, (2) for assessing the quality of proteins in detergent solubilization studies, and (3) for the detection of conformational changes in membrane proteins.

Original languageEnglish
Pages (from-to)12117-12124
Number of pages8
JournalBiochemistry
Volume45
Issue number39
DOIs
Publication statusPublished - Oct 3 2006

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Electrospray ionization
Peptide Mapping
Electrospray Ionization Mass Spectrometry
Liquid chromatography
Erythrocyte Membrane
Liquid Chromatography
Methionine
Mass spectrometry
Membrane Proteins
Peptides
Detergents
Oxidation
Peroxides
Conformations
Diethyl Pyrocarbonate
Erythrocyte Anion Exchange Protein 1
Anions
Proteins
Acids

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Identification of oxidized methionine sites in erythrocyte membrane protein by liquid chromatography/electrospray ionization mass spectrometry peptide mapping. / Li, Chunyan; Takazaki, Shinya; Jin, Xiuri; Kang, Dongchon; Abe, Yoshito; Hamasaki, Naotaka.

In: Biochemistry, Vol. 45, No. 39, 03.10.2006, p. 12117-12124.

Research output: Contribution to journalArticle

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