Identification of peroxisomal protein complexes with PTS receptors, pex5 and pex7, in mammalian cells

Kanji Okumoto, Non Miyata, Yukio Fujiki

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Pex5 and Pex7 are cytosolic receptors for peroxisome targeting signal type-1 (PTS1) and type-2 (PTS2), respectively, and play a pivotal role in import of peroxisomal matrix proteins. Recent advance in mass spectrometry analysis has facilitated comprehensive analysis of protein-protein interaction network by a combination with immunoprecipitation or biochemical purification. In this chapter, we introduce several findings obtained by these methods applied to mammalian cells. Exploring Pex5-binding partners in mammalian cells revealed core components comprising the import machinery complex of matrix proteins and a number of PTS1-type cargo proteins. Biochemical purification of the Pex5-export stimulating factor from rat liver cytosol fraction identified Awp1, providing further insight into molecular mechanisms of the export step of mono-ubiquitinated Pex5. Identification of DDB1 (damage-specific DNA-binding protein 1), a component of CRL4 (Cullin4A-RING ubiquitin ligase) E3 complex, as a Pex7-interacting protein revealed that quality control of Pex7 by CRL4A is important for PTS2 protein import by preventing the accumulation of dysfunctional Pex7. Furthermore, analysis of binding partners of an intraperoxisomal processing enzyme, trypsin-domain containing 1 (Tysnd1), showed a protein network regulating peroxisomal fatty acid β-oxidation.

Original languageEnglish
Title of host publicationSubcellular Biochemistry
PublisherSpringer New York
Pages287-298
Number of pages12
DOIs
Publication statusPublished - Jan 1 2018

Publication series

NameSubcellular Biochemistry
Volume89
ISSN (Print)0306-0225

Fingerprint

Cells
Proteins
Purification
Protein Interaction Maps
Peroxisomes
Ubiquitin-Protein Ligases
DNA-Binding Proteins
peroxisomal targeting sequence receptor
Immunoprecipitation
Quality Control
Cytosol
Trypsin
Mass Spectrometry
Liver
Fatty Acids
Machinery
Quality control
Mass spectrometry
Rats
Oxidation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology
  • Cancer Research

Cite this

Okumoto, K., Miyata, N., & Fujiki, Y. (2018). Identification of peroxisomal protein complexes with PTS receptors, pex5 and pex7, in mammalian cells. In Subcellular Biochemistry (pp. 287-298). (Subcellular Biochemistry; Vol. 89). Springer New York. https://doi.org/10.1007/978-981-13-2233-4_12

Identification of peroxisomal protein complexes with PTS receptors, pex5 and pex7, in mammalian cells. / Okumoto, Kanji; Miyata, Non; Fujiki, Yukio.

Subcellular Biochemistry. Springer New York, 2018. p. 287-298 (Subcellular Biochemistry; Vol. 89).

Research output: Chapter in Book/Report/Conference proceedingChapter

Okumoto, K, Miyata, N & Fujiki, Y 2018, Identification of peroxisomal protein complexes with PTS receptors, pex5 and pex7, in mammalian cells. in Subcellular Biochemistry. Subcellular Biochemistry, vol. 89, Springer New York, pp. 287-298. https://doi.org/10.1007/978-981-13-2233-4_12
Okumoto, Kanji ; Miyata, Non ; Fujiki, Yukio. / Identification of peroxisomal protein complexes with PTS receptors, pex5 and pex7, in mammalian cells. Subcellular Biochemistry. Springer New York, 2018. pp. 287-298 (Subcellular Biochemistry).
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