Identification of polyethylene glycol-resistant macrophages on stealth imaging in vitro using fluorescent organosilica nanoparticles

Michihiro Nakamura, Koichiro Hayashi, Mutsuki Nakano, Takafumi Kanadani, Kazue Miyamoto, Toshinari Kori, Kazuki Horikawa

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

An in vitro imaging system to evaluate the stealth function of nanoparticles against mouse macrophages was established using fluorescent organosilica nanoparticles. Surface-functionalized organosilica nanoparticles with polyethylene glycol (PEG) were prepared by a one-step process, resulting in a brush-type PEG layer. A simultaneous dual-particle administration approach enabled us to evaluate the stealth function of nanoparticles with respect to single cells using time-lapse fluorescent microscopic imaging and flow cytometry analyses. Single-cell imaging and analysis revealed various patterns and kinetics of bare and PEGylated nanoparticle uptake. The PEGylated nanoparticles revealed a stealth function against most macrophages (PEG-sensitive macrophages); however, a stealth function against certain macrophages (PEG-insensitive macrophages) was not observed. We identified and characterized the PEG-resistant macrophages that could take up PEGylated nanoparticles at the same level as bare nanoparticles.

Original languageEnglish
Pages (from-to)1058-1071
Number of pages14
JournalACS Nano
Volume9
Issue number2
DOIs
Publication statusPublished - Feb 24 2015
Externally publishedYes

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macrophages
Macrophages
Polyethylene glycols
glycols
polyethylenes
Nanoparticles
Imaging techniques
nanoparticles
cytometry
Flow cytometry
brushes
Brushes
cells
Imaging systems
mice
Kinetics
kinetics

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Identification of polyethylene glycol-resistant macrophages on stealth imaging in vitro using fluorescent organosilica nanoparticles. / Nakamura, Michihiro; Hayashi, Koichiro; Nakano, Mutsuki; Kanadani, Takafumi; Miyamoto, Kazue; Kori, Toshinari; Horikawa, Kazuki.

In: ACS Nano, Vol. 9, No. 2, 24.02.2015, p. 1058-1071.

Research output: Contribution to journalArticle

Nakamura, Michihiro ; Hayashi, Koichiro ; Nakano, Mutsuki ; Kanadani, Takafumi ; Miyamoto, Kazue ; Kori, Toshinari ; Horikawa, Kazuki. / Identification of polyethylene glycol-resistant macrophages on stealth imaging in vitro using fluorescent organosilica nanoparticles. In: ACS Nano. 2015 ; Vol. 9, No. 2. pp. 1058-1071.
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