Identification of polyphenols that repair the ultraviolet-B-induced DNA damage via SIRT1-dependent XPC/XPA activation

Zhao Chong, Haruka Matsuo, Shiori Onoue, Hiroaki Yamamoto, Hideyuki Ito, Yoshinori Katakura

Research output: Contribution to journalArticle

Abstract

Ultraviolet (UV)-B is an established etiological cause of skin damage. SIRT1, a mammalian SIR2 homolog localized in the nucleus and cytosol, plays a protective role in UVB-induced DNA damage. In this study, we established a method of screening foods and food ingredients, which augment the SIRT1 promoter in HaCaT cells, where we used recombinant HaCaT cells transduced with the vector expressing EGFP under the control of SIRT1 promoter. We identified four SIRT1-augmenting pomegranate-derived polyphenols (ellagic acid, punicalin, punicalagin, and urolithin A). All of these contributed to the proliferation of UVB-irradiated HaCaT cells. Punicalagin and urolithin A removed UVB-induced cyclobutane pyrimidine dimers (CPD) by activating nucleotide excision repair (NER). Both punicalagin and urolithin A upregulated NER-related XPC expression and XPA deacetylation level in a SIRT1-dependent manner. In the present study, we attempted to elucidate the mechanisms underlying the repair of UVB-damaged HaCaT cells by pomegranate-derived polyphenols.

Original languageEnglish
Pages (from-to)119-127
Number of pages9
JournalJournal of Functional Foods
Volume54
DOIs
Publication statusPublished - Mar 1 2019

Fingerprint

Polyphenols
DNA damage
DNA Damage
ultraviolet radiation
polyphenols
Punicaceae
DNA Repair
DNA repair
Ellagic Acid
Pyrimidine Dimers
Food
promoter regions
cells
ellagic acid
Cytosol
pyrimidines
cytosol
ingredients
Skin
screening

All Science Journal Classification (ASJC) codes

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Identification of polyphenols that repair the ultraviolet-B-induced DNA damage via SIRT1-dependent XPC/XPA activation. / Chong, Zhao; Matsuo, Haruka; Onoue, Shiori; Yamamoto, Hiroaki; Ito, Hideyuki; Katakura, Yoshinori.

In: Journal of Functional Foods, Vol. 54, 01.03.2019, p. 119-127.

Research output: Contribution to journalArticle

Chong, Zhao ; Matsuo, Haruka ; Onoue, Shiori ; Yamamoto, Hiroaki ; Ito, Hideyuki ; Katakura, Yoshinori. / Identification of polyphenols that repair the ultraviolet-B-induced DNA damage via SIRT1-dependent XPC/XPA activation. In: Journal of Functional Foods. 2019 ; Vol. 54. pp. 119-127.
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