TY - JOUR
T1 - Identification of polyphenols that repair the ultraviolet-B-induced DNA damage via SIRT1-dependent XPC/XPA activation
AU - Chong, Zhao
AU - Matsuo, Haruka
AU - Onoue, Shiori
AU - Yamamoto, Hiroaki
AU - Ito, Hideyuki
AU - Katakura, Yoshinori
N1 - Funding Information:
The authors would like to thank N. Oshima (GE Healthcare) for her expert assistance with the IN Cell Analyzer 1000. This study was partially supported by the Japan Science and Technology-Agency Japan International Cooperation Agency’s (JST-JICA) Science and Technology Research Partnership for Sustainable Development (SATREPS) Project. The funding source had no role in study design; in the collection, analysis and interpretation of data; in the writing of the report; and in the decision to submit the article for publication.
Publisher Copyright:
© 2019
PY - 2019/3
Y1 - 2019/3
N2 - Ultraviolet (UV)-B is an established etiological cause of skin damage. SIRT1, a mammalian SIR2 homolog localized in the nucleus and cytosol, plays a protective role in UVB-induced DNA damage. In this study, we established a method of screening foods and food ingredients, which augment the SIRT1 promoter in HaCaT cells, where we used recombinant HaCaT cells transduced with the vector expressing EGFP under the control of SIRT1 promoter. We identified four SIRT1-augmenting pomegranate-derived polyphenols (ellagic acid, punicalin, punicalagin, and urolithin A). All of these contributed to the proliferation of UVB-irradiated HaCaT cells. Punicalagin and urolithin A removed UVB-induced cyclobutane pyrimidine dimers (CPD) by activating nucleotide excision repair (NER). Both punicalagin and urolithin A upregulated NER-related XPC expression and XPA deacetylation level in a SIRT1-dependent manner. In the present study, we attempted to elucidate the mechanisms underlying the repair of UVB-damaged HaCaT cells by pomegranate-derived polyphenols.
AB - Ultraviolet (UV)-B is an established etiological cause of skin damage. SIRT1, a mammalian SIR2 homolog localized in the nucleus and cytosol, plays a protective role in UVB-induced DNA damage. In this study, we established a method of screening foods and food ingredients, which augment the SIRT1 promoter in HaCaT cells, where we used recombinant HaCaT cells transduced with the vector expressing EGFP under the control of SIRT1 promoter. We identified four SIRT1-augmenting pomegranate-derived polyphenols (ellagic acid, punicalin, punicalagin, and urolithin A). All of these contributed to the proliferation of UVB-irradiated HaCaT cells. Punicalagin and urolithin A removed UVB-induced cyclobutane pyrimidine dimers (CPD) by activating nucleotide excision repair (NER). Both punicalagin and urolithin A upregulated NER-related XPC expression and XPA deacetylation level in a SIRT1-dependent manner. In the present study, we attempted to elucidate the mechanisms underlying the repair of UVB-damaged HaCaT cells by pomegranate-derived polyphenols.
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U2 - 10.1016/j.jff.2019.01.017
DO - 10.1016/j.jff.2019.01.017
M3 - Article
AN - SCOPUS:85059957155
SN - 1756-4646
VL - 54
SP - 119
EP - 127
JO - Journal of Functional Foods
JF - Journal of Functional Foods
ER -