Identification of putative downstream genes of Oct-3, a pluripotent cell-specific transcription factor

Yukio Saijoh, Hideta Fujii, Chikara Meno, Mayumi Sato, Yoshihiro Hirota, Shinya Nagamatsu, Masako Ikeda, Hiroshi Hamada

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Background: Oct-3, a pluripotent cell-specific POU transcription factor, appears to be a key regulator in pluripotential early embryonic cells and germ cells. In order to study how pluripotency is maintained, it is essential to know what genes are regulated by Oct-3. Results: By employing a subtraction method, we identified several pluripotent cell-specific genes. Based upon expression patterns in various cell lines lacking or possessing Oct-3 function, about half of the genes were placed downstream of Oct-3. These downstream genes included a previously-known gene (Glut-3: a gene for a glucose transporter) and novel genes (226, 383 and 880). Their expression patterns paralleled that of Oct-3: all of these genes were highly expressed in pluripotent cells such as EC/ES cells, but switched off upon differentiation. More importantly, their expression was rescued in 'revertant' cells that ectopically acquired the Oct-3 transactivating function. Furthermore, the expression profiles of Glut-3, 226 and 383 during mouse development also overlapped that of Oct-3. The Glut-3 gene possessed multiple Oct-3 binding sites in its transcriptional regulatory regions, suggesting that at least one of the downstream genes was a direct target of Oct-3. Conclusions: A large proportion of pluripotent cell-specific genes appear to be downstream targets of Oct-3.

Original languageEnglish
Pages (from-to)239-252
Number of pages14
JournalGenes to Cells
Volume1
Issue number2
DOIs
Publication statusPublished - Jan 1 1996

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Transcription Factors
Genes
POU Domain Factors
Facilitative Glucose Transport Proteins
Nucleic Acid Regulatory Sequences
Germ Cells
Binding Sites
Cell Line

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

Identification of putative downstream genes of Oct-3, a pluripotent cell-specific transcription factor. / Saijoh, Yukio; Fujii, Hideta; Meno, Chikara; Sato, Mayumi; Hirota, Yoshihiro; Nagamatsu, Shinya; Ikeda, Masako; Hamada, Hiroshi.

In: Genes to Cells, Vol. 1, No. 2, 01.01.1996, p. 239-252.

Research output: Contribution to journalArticle

Saijoh, Yukio ; Fujii, Hideta ; Meno, Chikara ; Sato, Mayumi ; Hirota, Yoshihiro ; Nagamatsu, Shinya ; Ikeda, Masako ; Hamada, Hiroshi. / Identification of putative downstream genes of Oct-3, a pluripotent cell-specific transcription factor. In: Genes to Cells. 1996 ; Vol. 1, No. 2. pp. 239-252.
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