Identification of Quantitative Trait Loci for Resistance to RSIVD in Red Sea Bream (Pagrus major)

Eitaro Sawayama, Shiho Tanizawa, Shin Ichi Kitamura, Kei Nakayama, Kohei Ohta, Akiyuki Ozaki, Motohiro Takagi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Red sea bream iridoviral disease (RSIVD) is a major viral disease in red sea bream farming in Japan. Previously, we identified one candidate male individual of red sea bream that was significantly associated with convalescent individuals after RSIVD. The purpose of this study is to identify the quantitative trait loci (QTL) linked to the RSIVD-resistant trait for future marker-assisted selection (MAS). Two test families were developed using the candidate male in 2014 (Fam-2014) and 2015 (Fam-2015). These test families were challenged with RSIV, and phenotypes were evaluated. Then, de novo genome sequences of red sea bream were obtained through next-generation sequencing, and microsatellite markers were searched and selected for linkage map construction. One immune-related gene, MHC class IIβ, was also used for linkage map construction. Of the microsatellite markers searched, 148 and 197 were mapped on 23 and 27 linkage groups in the female and male linkage maps, respectively, covering approximately 65% of genomes in both sexes. One QTL linked to an RSIVD-resistant trait was found in linkage group 2 of the candidate male in Fam-2014, and the phenotypic variance of the QTL was 31.1%. The QTL was closely linked to MHC class IIβ. Moreover, the QTL observed in Fam-2014 was also significantly linked to an RSIVD-resistant trait in the candidate male of Fam-2015. Our results suggest that the RSIVD-resistant trait in the candidate male was controlled by one major QTL closely linked to the MHC class IIβ gene and could be useful for MAS of red sea bream.

Original languageEnglish
Pages (from-to)601-613
Number of pages13
JournalMarine Biotechnology
Volume19
Issue number6
DOIs
Publication statusPublished - Dec 1 2017

Fingerprint

Sea Bream
Indian Ocean
Pagrus major
Quantitative Trait Loci
quantitative trait loci
chromosome mapping
MHC Class II Genes
linkage groups
marker-assisted selection
Microsatellite Repeats
genome
sea
Genome
microsatellite repeats
viral disease
gene
Virus Diseases
Agriculture
phenotypic variation
phenotype

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Aquatic Science

Cite this

Identification of Quantitative Trait Loci for Resistance to RSIVD in Red Sea Bream (Pagrus major). / Sawayama, Eitaro; Tanizawa, Shiho; Kitamura, Shin Ichi; Nakayama, Kei; Ohta, Kohei; Ozaki, Akiyuki; Takagi, Motohiro.

In: Marine Biotechnology, Vol. 19, No. 6, 01.12.2017, p. 601-613.

Research output: Contribution to journalArticle

Sawayama, E, Tanizawa, S, Kitamura, SI, Nakayama, K, Ohta, K, Ozaki, A & Takagi, M 2017, 'Identification of Quantitative Trait Loci for Resistance to RSIVD in Red Sea Bream (Pagrus major)', Marine Biotechnology, vol. 19, no. 6, pp. 601-613. https://doi.org/10.1007/s10126-017-9779-z
Sawayama, Eitaro ; Tanizawa, Shiho ; Kitamura, Shin Ichi ; Nakayama, Kei ; Ohta, Kohei ; Ozaki, Akiyuki ; Takagi, Motohiro. / Identification of Quantitative Trait Loci for Resistance to RSIVD in Red Sea Bream (Pagrus major). In: Marine Biotechnology. 2017 ; Vol. 19, No. 6. pp. 601-613.
@article{e514fe343c564e8b9ce8d61196b89ce8,
title = "Identification of Quantitative Trait Loci for Resistance to RSIVD in Red Sea Bream (Pagrus major)",
abstract = "Red sea bream iridoviral disease (RSIVD) is a major viral disease in red sea bream farming in Japan. Previously, we identified one candidate male individual of red sea bream that was significantly associated with convalescent individuals after RSIVD. The purpose of this study is to identify the quantitative trait loci (QTL) linked to the RSIVD-resistant trait for future marker-assisted selection (MAS). Two test families were developed using the candidate male in 2014 (Fam-2014) and 2015 (Fam-2015). These test families were challenged with RSIV, and phenotypes were evaluated. Then, de novo genome sequences of red sea bream were obtained through next-generation sequencing, and microsatellite markers were searched and selected for linkage map construction. One immune-related gene, MHC class IIβ, was also used for linkage map construction. Of the microsatellite markers searched, 148 and 197 were mapped on 23 and 27 linkage groups in the female and male linkage maps, respectively, covering approximately 65{\%} of genomes in both sexes. One QTL linked to an RSIVD-resistant trait was found in linkage group 2 of the candidate male in Fam-2014, and the phenotypic variance of the QTL was 31.1{\%}. The QTL was closely linked to MHC class IIβ. Moreover, the QTL observed in Fam-2014 was also significantly linked to an RSIVD-resistant trait in the candidate male of Fam-2015. Our results suggest that the RSIVD-resistant trait in the candidate male was controlled by one major QTL closely linked to the MHC class IIβ gene and could be useful for MAS of red sea bream.",
author = "Eitaro Sawayama and Shiho Tanizawa and Kitamura, {Shin Ichi} and Kei Nakayama and Kohei Ohta and Akiyuki Ozaki and Motohiro Takagi",
year = "2017",
month = "12",
day = "1",
doi = "10.1007/s10126-017-9779-z",
language = "English",
volume = "19",
pages = "601--613",
journal = "Marine Biotechnology",
issn = "1436-2228",
publisher = "Springer New York",
number = "6",

}

TY - JOUR

T1 - Identification of Quantitative Trait Loci for Resistance to RSIVD in Red Sea Bream (Pagrus major)

AU - Sawayama, Eitaro

AU - Tanizawa, Shiho

AU - Kitamura, Shin Ichi

AU - Nakayama, Kei

AU - Ohta, Kohei

AU - Ozaki, Akiyuki

AU - Takagi, Motohiro

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Red sea bream iridoviral disease (RSIVD) is a major viral disease in red sea bream farming in Japan. Previously, we identified one candidate male individual of red sea bream that was significantly associated with convalescent individuals after RSIVD. The purpose of this study is to identify the quantitative trait loci (QTL) linked to the RSIVD-resistant trait for future marker-assisted selection (MAS). Two test families were developed using the candidate male in 2014 (Fam-2014) and 2015 (Fam-2015). These test families were challenged with RSIV, and phenotypes were evaluated. Then, de novo genome sequences of red sea bream were obtained through next-generation sequencing, and microsatellite markers were searched and selected for linkage map construction. One immune-related gene, MHC class IIβ, was also used for linkage map construction. Of the microsatellite markers searched, 148 and 197 were mapped on 23 and 27 linkage groups in the female and male linkage maps, respectively, covering approximately 65% of genomes in both sexes. One QTL linked to an RSIVD-resistant trait was found in linkage group 2 of the candidate male in Fam-2014, and the phenotypic variance of the QTL was 31.1%. The QTL was closely linked to MHC class IIβ. Moreover, the QTL observed in Fam-2014 was also significantly linked to an RSIVD-resistant trait in the candidate male of Fam-2015. Our results suggest that the RSIVD-resistant trait in the candidate male was controlled by one major QTL closely linked to the MHC class IIβ gene and could be useful for MAS of red sea bream.

AB - Red sea bream iridoviral disease (RSIVD) is a major viral disease in red sea bream farming in Japan. Previously, we identified one candidate male individual of red sea bream that was significantly associated with convalescent individuals after RSIVD. The purpose of this study is to identify the quantitative trait loci (QTL) linked to the RSIVD-resistant trait for future marker-assisted selection (MAS). Two test families were developed using the candidate male in 2014 (Fam-2014) and 2015 (Fam-2015). These test families were challenged with RSIV, and phenotypes were evaluated. Then, de novo genome sequences of red sea bream were obtained through next-generation sequencing, and microsatellite markers were searched and selected for linkage map construction. One immune-related gene, MHC class IIβ, was also used for linkage map construction. Of the microsatellite markers searched, 148 and 197 were mapped on 23 and 27 linkage groups in the female and male linkage maps, respectively, covering approximately 65% of genomes in both sexes. One QTL linked to an RSIVD-resistant trait was found in linkage group 2 of the candidate male in Fam-2014, and the phenotypic variance of the QTL was 31.1%. The QTL was closely linked to MHC class IIβ. Moreover, the QTL observed in Fam-2014 was also significantly linked to an RSIVD-resistant trait in the candidate male of Fam-2015. Our results suggest that the RSIVD-resistant trait in the candidate male was controlled by one major QTL closely linked to the MHC class IIβ gene and could be useful for MAS of red sea bream.

UR - http://www.scopus.com/inward/record.url?scp=85033435854&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85033435854&partnerID=8YFLogxK

U2 - 10.1007/s10126-017-9779-z

DO - 10.1007/s10126-017-9779-z

M3 - Article

VL - 19

SP - 601

EP - 613

JO - Marine Biotechnology

JF - Marine Biotechnology

SN - 1436-2228

IS - 6

ER -