Identification of Ralstonia solanacearum Isolated from Wilted Tobacco Plant by Fatty Acid Profiles and PCR-RFLP Analysis

Abu Ashraf Khan, Naruto Furuya, Matsumoto Masaru, Nobuaki Matsuyama

Research output: Contribution to journalArticle

Abstract

Identification of Ralstonia solanacearum, isolated from wilted tobacco plant was conducted by fatty acid profiles and PCR-RFLP analysis. The tobacco strain showed identical patterns of fatty acid composition with Ralstonia solanacearum and R. pickettii, and it was distinctly different from Burkholderia gladioli pv. gladioli, B. cepacia and B. caryophylli. Palmitoleic acid (16:1 cis 9) content was much higher in tobacco strain, R. solanacearum and R. pickettii. On the other hand, an unidentified (unidentified-6) fatty acid was detected (9.55 to 11.98%) in B. gladioli pv. gladioli, B. cepacia and B. caryophylli, and it was absent in tobacco strain, R. solanacearum and R. pickettii. The tobacco strain showed identical PCR-RFLP profiles (MspI digestion) with R. solanacearum and R. pickettii. The PCR-RFLP profiles with HaeIII digestion further differentiated the tobacco strain and R. solanacearum from A. pickettii. Both fatty acid profiles and PCR-RFLP analysis suggested that tobacco strain is R. solanacearum. The physiological and biochemical tests also confirmed this conclusion.

Original languageEnglish
Pages (from-to)59-65
Number of pages7
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume44
Issue number1-2
Publication statusPublished - Nov 1 1999

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Ralstonia solanacearum
Restriction Fragment Length Polymorphisms
Tobacco
restriction fragment length polymorphism
tobacco
Fatty Acids
fatty acid composition
Polymerase Chain Reaction
Burkholderia gladioli
Gladiolus
Burkholderia caryophylli
Digestion
Ralstonia pickettii
digestion
Burkholderia cepacia
palmitoleic acid
fatty acids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

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title = "Identification of Ralstonia solanacearum Isolated from Wilted Tobacco Plant by Fatty Acid Profiles and PCR-RFLP Analysis",
abstract = "Identification of Ralstonia solanacearum, isolated from wilted tobacco plant was conducted by fatty acid profiles and PCR-RFLP analysis. The tobacco strain showed identical patterns of fatty acid composition with Ralstonia solanacearum and R. pickettii, and it was distinctly different from Burkholderia gladioli pv. gladioli, B. cepacia and B. caryophylli. Palmitoleic acid (16:1 cis 9) content was much higher in tobacco strain, R. solanacearum and R. pickettii. On the other hand, an unidentified (unidentified-6) fatty acid was detected (9.55 to 11.98{\%}) in B. gladioli pv. gladioli, B. cepacia and B. caryophylli, and it was absent in tobacco strain, R. solanacearum and R. pickettii. The tobacco strain showed identical PCR-RFLP profiles (MspI digestion) with R. solanacearum and R. pickettii. The PCR-RFLP profiles with HaeIII digestion further differentiated the tobacco strain and R. solanacearum from A. pickettii. Both fatty acid profiles and PCR-RFLP analysis suggested that tobacco strain is R. solanacearum. The physiological and biochemical tests also confirmed this conclusion.",
author = "Khan, {Abu Ashraf} and Naruto Furuya and Matsumoto Masaru and Nobuaki Matsuyama",
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T1 - Identification of Ralstonia solanacearum Isolated from Wilted Tobacco Plant by Fatty Acid Profiles and PCR-RFLP Analysis

AU - Khan, Abu Ashraf

AU - Furuya, Naruto

AU - Masaru, Matsumoto

AU - Matsuyama, Nobuaki

PY - 1999/11/1

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N2 - Identification of Ralstonia solanacearum, isolated from wilted tobacco plant was conducted by fatty acid profiles and PCR-RFLP analysis. The tobacco strain showed identical patterns of fatty acid composition with Ralstonia solanacearum and R. pickettii, and it was distinctly different from Burkholderia gladioli pv. gladioli, B. cepacia and B. caryophylli. Palmitoleic acid (16:1 cis 9) content was much higher in tobacco strain, R. solanacearum and R. pickettii. On the other hand, an unidentified (unidentified-6) fatty acid was detected (9.55 to 11.98%) in B. gladioli pv. gladioli, B. cepacia and B. caryophylli, and it was absent in tobacco strain, R. solanacearum and R. pickettii. The tobacco strain showed identical PCR-RFLP profiles (MspI digestion) with R. solanacearum and R. pickettii. The PCR-RFLP profiles with HaeIII digestion further differentiated the tobacco strain and R. solanacearum from A. pickettii. Both fatty acid profiles and PCR-RFLP analysis suggested that tobacco strain is R. solanacearum. The physiological and biochemical tests also confirmed this conclusion.

AB - Identification of Ralstonia solanacearum, isolated from wilted tobacco plant was conducted by fatty acid profiles and PCR-RFLP analysis. The tobacco strain showed identical patterns of fatty acid composition with Ralstonia solanacearum and R. pickettii, and it was distinctly different from Burkholderia gladioli pv. gladioli, B. cepacia and B. caryophylli. Palmitoleic acid (16:1 cis 9) content was much higher in tobacco strain, R. solanacearum and R. pickettii. On the other hand, an unidentified (unidentified-6) fatty acid was detected (9.55 to 11.98%) in B. gladioli pv. gladioli, B. cepacia and B. caryophylli, and it was absent in tobacco strain, R. solanacearum and R. pickettii. The tobacco strain showed identical PCR-RFLP profiles (MspI digestion) with R. solanacearum and R. pickettii. The PCR-RFLP profiles with HaeIII digestion further differentiated the tobacco strain and R. solanacearum from A. pickettii. Both fatty acid profiles and PCR-RFLP analysis suggested that tobacco strain is R. solanacearum. The physiological and biochemical tests also confirmed this conclusion.

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