Identification of Ralstonia solanacearum Isolated from Wilted Tobacco Plant by Fatty Acid Profiles and PCR-RFLP Analysis

Abu Ashraf Khan, Naruto Furuya, Matsumoto Masaru, Nobuaki Matsuyama

Research output: Contribution to journalArticle

Abstract

Identification of Ralstonia solanacearum, isolated from wilted tobacco plant was conducted by fatty acid profiles and PCR-RFLP analysis. The tobacco strain showed identical patterns of fatty acid composition with Ralstonia solanacearum and R. pickettii, and it was distinctly different from Burkholderia gladioli pv. gladioli, B. cepacia and B. caryophylli. Palmitoleic acid (16:1 cis 9) content was much higher in tobacco strain, R. solanacearum and R. pickettii. On the other hand, an unidentified (unidentified-6) fatty acid was detected (9.55 to 11.98%) in B. gladioli pv. gladioli, B. cepacia and B. caryophylli, and it was absent in tobacco strain, R. solanacearum and R. pickettii. The tobacco strain showed identical PCR-RFLP profiles (MspI digestion) with R. solanacearum and R. pickettii. The PCR-RFLP profiles with HaeIII digestion further differentiated the tobacco strain and R. solanacearum from A. pickettii. Both fatty acid profiles and PCR-RFLP analysis suggested that tobacco strain is R. solanacearum. The physiological and biochemical tests also confirmed this conclusion.

Original languageEnglish
Pages (from-to)59-65
Number of pages7
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume44
Issue number1-2
Publication statusPublished - Nov 1 1999

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

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