Identification of recurrence-related microRNAs from bone marrow in hepatocellular carcinoma patients

Keishi Sugimachi; Shotaro Sakimura; Akira Tomokuni; Ryutaro Uchi; Hidenari Hirata; Hisateru Komatsu; Yoshiaki Shinden; Tomohiro Iguchi; Hidetoshi Eguchi; Takaaki Masuda; Kazutoyo Morita; Ken Shirabe; Hidetoshi Eguchi; Yoshihiko Maehara; Masaki Mori; Koshi Mimori

doi:10.3390/jcm4081600

Identification of recurrence-related microRNAs from bone marrow in hepatocellular carcinoma patients

Keishi Sugimachi, Shotaro Sakimura, Akira Tomokuni, Ryutaro Uchi, Hidenari Hirata, Hisateru Komatsu, Yoshiaki Shinden, Tomohiro Iguchi, Hidetoshi Eguchi, Takaaki Masuda, Kazutoyo Morita, Ken Shirabe, Hidetoshi Eguchi, Yoshihiko Maehara, Masaki Mori, Koshi Mimori

Research output: Contribution to journal › Comment/debate › peer-review

Abstract

Hepatocellular carcinoma (HCC) is a poor-prognosis cancer due to its high rate of recurrence. microRNAs (miRNAs) are a class of small non-coding RNA molecules that affect crucial processes in cancer development. The objective of this study is to identify the role of miRNAs in patient bone marrow (BM) and explore the function of these molecules during HCC progression. We purified miRNAs from bone marrow cells of seven HCC patients, and divided them into three fractions by cell surface markers as follows: CD14⁺ (macrophage), CD14⁻/CD45⁺ (lymphocyte), and CD14⁻/CD45⁻/EpCAM⁺ (epithelial cell). We employed microarray-based profiling to analyze miRNA expression in the bone marrow of patients with HCC. Differentially expressed miRNAs were significantly different between fractions from whole bone marrow, macrophages, and lymphocytes, and depended on stages in tumor progression. Differences in expression of miRNAs associated with cell proliferation also varied significantly between HCC patients with recurrence, multiple tumors, and advanced clinical stages. These results suggest that miRNA profiles in separated fractions of BM cells are associated with HCC progression.

Original language	English
Pages (from-to)	1600-1611
Number of pages	12
Journal	Journal of Clinical Medicine
Volume	4
Issue number	8
DOIs	https://doi.org/10.3390/jcm4081600
Publication status	Published - Jul 14 2015

All Science Journal Classification (ASJC) codes

Medicine(all)

Access to Document

10.3390/jcm4081600

Fingerprint Dive into the research topics of 'Identification of recurrence-related microRNAs from bone marrow in hepatocellular carcinoma patients'. Together they form a unique fingerprint.

Cite this

Sugimachi, K., Sakimura, S., Tomokuni, A., Uchi, R., Hirata, H., Komatsu, H., Shinden, Y., Iguchi, T., Eguchi, H., Masuda, T., Morita, K., Shirabe, K., Eguchi, H., Maehara, Y., Mori, M., & Mimori, K. (2015). Identification of recurrence-related microRNAs from bone marrow in hepatocellular carcinoma patients. Journal of Clinical Medicine, 4(8), 1600-1611. https://doi.org/10.3390/jcm4081600

Identification of recurrence-related microRNAs from bone marrow in hepatocellular carcinoma patients. / Sugimachi, Keishi; Sakimura, Shotaro; Tomokuni, Akira; Uchi, Ryutaro; Hirata, Hidenari; Komatsu, Hisateru; Shinden, Yoshiaki; Iguchi, Tomohiro; Eguchi, Hidetoshi; Masuda, Takaaki; Morita, Kazutoyo; Shirabe, Ken; Eguchi, Hidetoshi; Maehara, Yoshihiko; Mori, Masaki; Mimori, Koshi.

In: Journal of Clinical Medicine, Vol. 4, No. 8, 14.07.2015, p. 1600-1611.

Research output: Contribution to journal › Comment/debate › peer-review

Sugimachi, K, Sakimura, S, Tomokuni, A, Uchi, R, Hirata, H, Komatsu, H, Shinden, Y, Iguchi, T, Eguchi, H, Masuda, T, Morita, K, Shirabe, K, Eguchi, H, Maehara, Y, Mori, M & Mimori, K 2015, 'Identification of recurrence-related microRNAs from bone marrow in hepatocellular carcinoma patients', Journal of Clinical Medicine, vol. 4, no. 8, pp. 1600-1611. https://doi.org/10.3390/jcm4081600

Sugimachi, Keishi ; Sakimura, Shotaro ; Tomokuni, Akira ; Uchi, Ryutaro ; Hirata, Hidenari ; Komatsu, Hisateru ; Shinden, Yoshiaki ; Iguchi, Tomohiro ; Eguchi, Hidetoshi ; Masuda, Takaaki ; Morita, Kazutoyo ; Shirabe, Ken ; Eguchi, Hidetoshi ; Maehara, Yoshihiko ; Mori, Masaki ; Mimori, Koshi. / Identification of recurrence-related microRNAs from bone marrow in hepatocellular carcinoma patients. In: Journal of Clinical Medicine. 2015 ; Vol. 4, No. 8. pp. 1600-1611.

@article{3cbffe9523254198ad0ff5cf571e19aa,

title = "Identification of recurrence-related microRNAs from bone marrow in hepatocellular carcinoma patients",

abstract = "Hepatocellular carcinoma (HCC) is a poor-prognosis cancer due to its high rate of recurrence. microRNAs (miRNAs) are a class of small non-coding RNA molecules that affect crucial processes in cancer development. The objective of this study is to identify the role of miRNAs in patient bone marrow (BM) and explore the function of these molecules during HCC progression. We purified miRNAs from bone marrow cells of seven HCC patients, and divided them into three fractions by cell surface markers as follows: CD14+ (macrophage), CD14−/CD45+ (lymphocyte), and CD14−/CD45−/EpCAM+ (epithelial cell). We employed microarray-based profiling to analyze miRNA expression in the bone marrow of patients with HCC. Differentially expressed miRNAs were significantly different between fractions from whole bone marrow, macrophages, and lymphocytes, and depended on stages in tumor progression. Differences in expression of miRNAs associated with cell proliferation also varied significantly between HCC patients with recurrence, multiple tumors, and advanced clinical stages. These results suggest that miRNA profiles in separated fractions of BM cells are associated with HCC progression.",

author = "Keishi Sugimachi and Shotaro Sakimura and Akira Tomokuni and Ryutaro Uchi and Hidenari Hirata and Hisateru Komatsu and Yoshiaki Shinden and Tomohiro Iguchi and Hidetoshi Eguchi and Takaaki Masuda and Kazutoyo Morita and Ken Shirabe and Hidetoshi Eguchi and Yoshihiko Maehara and Masaki Mori and Koshi Mimori",

year = "2015",

month = jul,

day = "14",

doi = "10.3390/jcm4081600",

language = "English",

volume = "4",

pages = "1600--1611",

journal = "Journal of Clinical Medicine",

issn = "2077-0383",

publisher = "MDPI AG",

number = "8",

}

TY - JOUR

T1 - Identification of recurrence-related microRNAs from bone marrow in hepatocellular carcinoma patients

AU - Sugimachi, Keishi

AU - Sakimura, Shotaro

AU - Tomokuni, Akira

AU - Uchi, Ryutaro

AU - Hirata, Hidenari

AU - Komatsu, Hisateru

AU - Shinden, Yoshiaki

AU - Iguchi, Tomohiro

AU - Eguchi, Hidetoshi

AU - Masuda, Takaaki

AU - Morita, Kazutoyo

AU - Shirabe, Ken

AU - Eguchi, Hidetoshi

AU - Maehara, Yoshihiko

AU - Mori, Masaki

AU - Mimori, Koshi

PY - 2015/7/14

Y1 - 2015/7/14

N2 - Hepatocellular carcinoma (HCC) is a poor-prognosis cancer due to its high rate of recurrence. microRNAs (miRNAs) are a class of small non-coding RNA molecules that affect crucial processes in cancer development. The objective of this study is to identify the role of miRNAs in patient bone marrow (BM) and explore the function of these molecules during HCC progression. We purified miRNAs from bone marrow cells of seven HCC patients, and divided them into three fractions by cell surface markers as follows: CD14+ (macrophage), CD14−/CD45+ (lymphocyte), and CD14−/CD45−/EpCAM+ (epithelial cell). We employed microarray-based profiling to analyze miRNA expression in the bone marrow of patients with HCC. Differentially expressed miRNAs were significantly different between fractions from whole bone marrow, macrophages, and lymphocytes, and depended on stages in tumor progression. Differences in expression of miRNAs associated with cell proliferation also varied significantly between HCC patients with recurrence, multiple tumors, and advanced clinical stages. These results suggest that miRNA profiles in separated fractions of BM cells are associated with HCC progression.

AB - Hepatocellular carcinoma (HCC) is a poor-prognosis cancer due to its high rate of recurrence. microRNAs (miRNAs) are a class of small non-coding RNA molecules that affect crucial processes in cancer development. The objective of this study is to identify the role of miRNAs in patient bone marrow (BM) and explore the function of these molecules during HCC progression. We purified miRNAs from bone marrow cells of seven HCC patients, and divided them into three fractions by cell surface markers as follows: CD14+ (macrophage), CD14−/CD45+ (lymphocyte), and CD14−/CD45−/EpCAM+ (epithelial cell). We employed microarray-based profiling to analyze miRNA expression in the bone marrow of patients with HCC. Differentially expressed miRNAs were significantly different between fractions from whole bone marrow, macrophages, and lymphocytes, and depended on stages in tumor progression. Differences in expression of miRNAs associated with cell proliferation also varied significantly between HCC patients with recurrence, multiple tumors, and advanced clinical stages. These results suggest that miRNA profiles in separated fractions of BM cells are associated with HCC progression.

UR - http://www.scopus.com/inward/record.url?scp=85030463570&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85030463570&partnerID=8YFLogxK

U2 - 10.3390/jcm4081600

DO - 10.3390/jcm4081600

M3 - Comment/debate

VL - 4

SP - 1600

EP - 1611

JO - Journal of Clinical Medicine

JF - Journal of Clinical Medicine

SN - 2077-0383

IS - 8

ER -