Identification of reference genes for quantitative PCR analyses in developing mouse gonads

Toshifumi Yokoyama, Takuya Omotehara, Tetsushi Hirano, Naoto Kubota, Shogo Yanai, Chinatsu Hasegawa, Tadashi Takada, Yohei Mantani, Nobuhiko Hoshi

Research output: Contribution to journalComment/debatepeer-review

Abstract

Stable reference genes are important for gene expression analyses such as quantitative PCR. The stability of 15 candidate reference genes that can be used to developing mouse gonads was thoroughly verified using combinations of multiple algorithms. The expression of these genes fluctuated greatly depending on the analysis period and/or gender. Peptidylprolyl isomerase A (Ppia) and polymerase (RNA) II (DNA directed) polypeptide A (Polr2a) were the reference genes that were used stably for a wide analysis period in developing mouse gonads. Furthermore, the stable reference genes corresponding to the analysis period and/or gender were ranked. These results are useful for the selection of the optimal reference gene required for high-precision measurements.

Original languageEnglish
Pages (from-to)1534-1539
Number of pages6
JournalJournal of Veterinary Medical Science
Volume80
Issue number10
DOIs
Publication statusPublished - 2018
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • veterinary(all)

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