Identification of single molecular DNA methylation points by microfluidic DNA molecule stretching and quantum dot detection

Yukihiro Okamoto, Tatsuki Sano, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We report a rapid and simple epigenetic analysis based on microfluidic single DNA molecular methylation detection. We developed a microfluidic device to stretch a single DNA molecule and subsequent detection of a methylated DNA base by quantum dot (QD)-immobilized methyl binding domain (MBD) protein. A QD-MBD complex specifically bound to a methylated base of stretched DNA and was detected by a total internal reflection fluorescence microscope (TIRFM). Our method enables us to detect a number of methylation site and identify the methylated positions simultaneously in the simple and rapid manner compared with the conventional methods.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1939-1941
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - Jan 1 2012
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: Oct 28 2012Nov 1 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period10/28/1211/1/12

Fingerprint

Microfluidics
Semiconductor quantum dots
Stretching
DNA
Molecules
Methylation
Microscopes
Fluorescence
Proteins
DNA Methylation

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Okamoto, Y., Sano, T., Kaji, N., Tokeshi, M., & Baba, Y. (2012). Identification of single molecular DNA methylation points by microfluidic DNA molecule stretching and quantum dot detection. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1939-1941). Chemical and Biological Microsystems Society.

Identification of single molecular DNA methylation points by microfluidic DNA molecule stretching and quantum dot detection. / Okamoto, Yukihiro; Sano, Tatsuki; Kaji, Noritada; Tokeshi, Manabu; Baba, Yoshinobu.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 1939-1941.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Okamoto, Y, Sano, T, Kaji, N, Tokeshi, M & Baba, Y 2012, Identification of single molecular DNA methylation points by microfluidic DNA molecule stretching and quantum dot detection. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, pp. 1939-1941, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 10/28/12.
Okamoto Y, Sano T, Kaji N, Tokeshi M, Baba Y. Identification of single molecular DNA methylation points by microfluidic DNA molecule stretching and quantum dot detection. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 1939-1941
Okamoto, Yukihiro ; Sano, Tatsuki ; Kaji, Noritada ; Tokeshi, Manabu ; Baba, Yoshinobu. / Identification of single molecular DNA methylation points by microfluidic DNA molecule stretching and quantum dot detection. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 1939-1941
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