Identification of the cytochrome P450 involved in the degradation of neonicotinoid insecticide acetamiprid in Phanerochaete chrysosporium

Jianqiao Wang, Haruka Ohno, Yuuri Ide, Hirofumi Ichinose, Toshio Mori, Hirokazu Kawagishi, Hirofumi Hirai

Research output: Contribution to journalArticle

Abstract

We previously reported that cytochrome P450 s play critical roles in neonicotinoid insecticide biodegradation by white-rot fungi. Here, we investigated the biodegradation of acetamiprid (ACET) by Phanerochaete chrysosporium to identify the cytochrome P450 involved in this degradation process. During a 20-day incubation period, P. chrysosporium degraded 21% and 51% of ACET in ligninolytic and nonligninolytic media, respectively. The degradation rate of ACET was markedly decreased by the addition of cytochrome P450 inhibitors. Recombinant cytochrome P450s in P. chrysosporium (PcCYP) were heterologously expressed in Saccharomyces cerevisiae strain AH22, and the PcCYP involved in ACET degradation was identified. The results showed that CYP5147A3 can degrade ACET, and two ACET metabolites, N’-cyano-N-methyl acetamidine and 6-chloro-3-pyridinemethanol, were identified. To the best of our knowledge, this study provides the first characterization of the fungal cytochrome P450 that is responsible for the degradation and detoxification of ACET.

Original languageEnglish
Pages (from-to)494-498
Number of pages5
JournalJournal of Hazardous Materials
Volume371
DOIs
Publication statusPublished - Jun 5 2019

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Phanerochaete
Insecticides
Cytochrome P-450 Enzyme System
insecticide
cytochrome
Degradation
degradation
Biodegradation
biodegradation
Detoxification
Metabolites
Fungi
Nicotinyl Alcohol
Yeast
detoxification
inhibitor
Proteins
metabolite
incubation
Cytochromes

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

Identification of the cytochrome P450 involved in the degradation of neonicotinoid insecticide acetamiprid in Phanerochaete chrysosporium. / Wang, Jianqiao; Ohno, Haruka; Ide, Yuuri; Ichinose, Hirofumi; Mori, Toshio; Kawagishi, Hirokazu; Hirai, Hirofumi.

In: Journal of Hazardous Materials, Vol. 371, 05.06.2019, p. 494-498.

Research output: Contribution to journalArticle

Wang, Jianqiao ; Ohno, Haruka ; Ide, Yuuri ; Ichinose, Hirofumi ; Mori, Toshio ; Kawagishi, Hirokazu ; Hirai, Hirofumi. / Identification of the cytochrome P450 involved in the degradation of neonicotinoid insecticide acetamiprid in Phanerochaete chrysosporium. In: Journal of Hazardous Materials. 2019 ; Vol. 371. pp. 494-498.
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