Identification of the disease- related T cell epitope of ovalbumin and epitope-targeted T cell inactivation in egg allergy

Naoki Shimojo, Toshiyitki Katsuki, John E. Coligan, Yasuharu Nishimura, Takehiko Sasazuki, Hajime Tsunoo, Tateo Sakamaki, Yoichi Kohno, Hiroo Niimi

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35 Citations (Scopus)

Abstract

An ovalbumin (OVA)-specifíc T cell line (TCL) was established from a patient with hen egg allergy. The TCL was CD4+, expressed αβ T cell receptor, and recognized OVA presented by HLA-DR10. Based on the response of the TCL to synthetic OVA peptides, it was found that the TCL recognized OVA 323-339, which is a major T cell epitope presented by murine I-Ad. The TCL secreted high levels of IL-5, but undetectable amounts of IL-2, interferon-γ, and IL-4 when stimulated with OVA or the OVA 323-339 peptide. Since IL-5 is an important growth and chemotactic factor for eosinophils, it is possible that these OVA 323- 339-specific T cells can contribute to human egg allergy. To our knowledge, this is the first demonstration of food allergen-specific TCL establishment and identification of a T cell epitope possibly related to the allergic reaction to food antigens. An analog peptide of the OVA 323-339 which is known to strongly bind to I-Ad partially inhibited the response of the TCL to OVA 323-339 presented by HLA-DR10, raising the possibility of peptide-based immunotherapy of food allergy.

Original languageEnglish
Pages (from-to)155-161
Number of pages7
JournalInternational Archives of Allergy and Immunology
Volume105
Issue number2
DOIs
Publication statusPublished - Jan 1 1994

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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    Shimojo, N., Katsuki, T., Coligan, J. E., Nishimura, Y., Sasazuki, T., Tsunoo, H., Sakamaki, T., Kohno, Y., & Niimi, H. (1994). Identification of the disease- related T cell epitope of ovalbumin and epitope-targeted T cell inactivation in egg allergy. International Archives of Allergy and Immunology, 105(2), 155-161. https://doi.org/10.1159/000236818