Identification of the disease- related T cell epitope of ovalbumin and epitope-targeted T cell inactivation in egg allergy

Naoki Shimojo, Toshiyitki Katsuki, John E. Coligan, Yasuharu Nishimura, Takehiko Sasazuki, Hajime Tsunoo, Tateo Sakamaki, Yoichi Kohno, Hiroo Niimi

Research output: Contribution to journalArticle

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Abstract

An ovalbumin (OVA)-specifíc T cell line (TCL) was established from a patient with hen egg allergy. The TCL was CD4+, expressed αβ T cell receptor, and recognized OVA presented by HLA-DR10. Based on the response of the TCL to synthetic OVA peptides, it was found that the TCL recognized OVA 323-339, which is a major T cell epitope presented by murine I-Ad. The TCL secreted high levels of IL-5, but undetectable amounts of IL-2, interferon-γ, and IL-4 when stimulated with OVA or the OVA 323-339 peptide. Since IL-5 is an important growth and chemotactic factor for eosinophils, it is possible that these OVA 323- 339-specific T cells can contribute to human egg allergy. To our knowledge, this is the first demonstration of food allergen-specific TCL establishment and identification of a T cell epitope possibly related to the allergic reaction to food antigens. An analog peptide of the OVA 323-339 which is known to strongly bind to I-Ad partially inhibited the response of the TCL to OVA 323-339 presented by HLA-DR10, raising the possibility of peptide-based immunotherapy of food allergy.

Original languageEnglish
Pages (from-to)155-161
Number of pages7
JournalInternational Archives of Allergy and Immunology
Volume105
Issue number2
DOIs
Publication statusPublished - Jan 1 1994

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Egg Hypersensitivity
T-Lymphocyte Epitopes
Ovalbumin
T-Lymphocytes
Cell Line
Interleukin-5
Food
Peptides
Food Hypersensitivity
Chemotactic Factors
T-Cell Antigen Receptor
Eosinophils
Interleukin-4
Immunotherapy
Allergens
Interferons
Interleukin-2
Intercellular Signaling Peptides and Proteins
Hypersensitivity
Antigens

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Identification of the disease- related T cell epitope of ovalbumin and epitope-targeted T cell inactivation in egg allergy. / Shimojo, Naoki; Katsuki, Toshiyitki; Coligan, John E.; Nishimura, Yasuharu; Sasazuki, Takehiko; Tsunoo, Hajime; Sakamaki, Tateo; Kohno, Yoichi; Niimi, Hiroo.

In: International Archives of Allergy and Immunology, Vol. 105, No. 2, 01.01.1994, p. 155-161.

Research output: Contribution to journalArticle

Shimojo, N, Katsuki, T, Coligan, JE, Nishimura, Y, Sasazuki, T, Tsunoo, H, Sakamaki, T, Kohno, Y & Niimi, H 1994, 'Identification of the disease- related T cell epitope of ovalbumin and epitope-targeted T cell inactivation in egg allergy', International Archives of Allergy and Immunology, vol. 105, no. 2, pp. 155-161. https://doi.org/10.1159/000236818
Shimojo, Naoki ; Katsuki, Toshiyitki ; Coligan, John E. ; Nishimura, Yasuharu ; Sasazuki, Takehiko ; Tsunoo, Hajime ; Sakamaki, Tateo ; Kohno, Yoichi ; Niimi, Hiroo. / Identification of the disease- related T cell epitope of ovalbumin and epitope-targeted T cell inactivation in egg allergy. In: International Archives of Allergy and Immunology. 1994 ; Vol. 105, No. 2. pp. 155-161.
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