TY - JOUR
T1 - Identification of the fnx1+ and fnx2+ genes for vacuolar amino acid transporters in Schizosaccharomyces pombe
AU - Chardwiriyapreecha, Soracom
AU - Shimazu, Masamitsu
AU - Morita, Tomotake
AU - Sekito, Takayuki
AU - Akiyama, Koichi
AU - Takegawa, Kaoru
AU - Kakinuma, Yoshimi
N1 - Funding Information:
This work was supported in part by Grant-in-Aid for Scientific Research (to Y.K.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. We thank Dr. Naotaka Tanaka, Kagawa University, for his technical help of fluorescence microscopy.
PY - 2008/6/25
Y1 - 2008/6/25
N2 - We have identified the Schizosaccharomyces pombe SPBC3E7.06c gene (fnx2+) from a homology search with the fnx1+ gene involving in G0 arrest upon nitrogen starvation. Green fluorescent protein-fused Fnx1p and Fnx2p localized exclusively to the vacuolar membrane. Uptake of histidine or isoleucine by S. pombe cells was inhibited by concanamycin A, a specific inhibitor of the vacuolar H+-ATPase. Amino acid uptake was also defective in the vacuolar ATPase mutant, suggesting that vacuolar compartmentalization is critical for amino acid uptake by whole cells. In both Δfnx1 and Δfnx2 mutant cells, uptake of lysine, isoleucine or asparagine was impaired. These results suggest that fnx1+ and fnx2+ are involved in vacuolar amino acid uptake in S. pombe.
AB - We have identified the Schizosaccharomyces pombe SPBC3E7.06c gene (fnx2+) from a homology search with the fnx1+ gene involving in G0 arrest upon nitrogen starvation. Green fluorescent protein-fused Fnx1p and Fnx2p localized exclusively to the vacuolar membrane. Uptake of histidine or isoleucine by S. pombe cells was inhibited by concanamycin A, a specific inhibitor of the vacuolar H+-ATPase. Amino acid uptake was also defective in the vacuolar ATPase mutant, suggesting that vacuolar compartmentalization is critical for amino acid uptake by whole cells. In both Δfnx1 and Δfnx2 mutant cells, uptake of lysine, isoleucine or asparagine was impaired. These results suggest that fnx1+ and fnx2+ are involved in vacuolar amino acid uptake in S. pombe.
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U2 - 10.1016/j.febslet.2008.05.017
DO - 10.1016/j.febslet.2008.05.017
M3 - Article
C2 - 18503766
AN - SCOPUS:44749083301
SN - 0014-5793
VL - 582
SP - 2225
EP - 2230
JO - FEBS Letters
JF - FEBS Letters
IS - 15
ER -