TY - JOUR
T1 - Identification of the goldfish 20S proteasome β6 subunit bound to nuclear matrix
AU - Tokumoto, Mika
AU - Yamaguchi, Akihiko
AU - Nagahama, Yoshitaka
AU - Tokumoto, Toshinobu
N1 - Funding Information:
A part of this work was supported by Grants-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Science, Sports and Culture of Japan. A part of this study was performed under the National Institute for Basic Biology Cooperative Research Program (99-121 to T.T.). M.T. is a Research Fellow of the Japan Society for the Promotion of Science.
PY - 2000/4/21
Y1 - 2000/4/21
N2 - Proteasomes are large, multisubunit particles that act as the proteolytic machinery for most of the regulated intracellular protein breakdown in eukaryotic cells. Proteasomes are present in both the nucleus and cytoplasm. When we analyzed the molecular composition of protein constituents of the nuclear matrix preparation of goldfish oocytes by two-dimensional polyacrylamide gel electrophoresis followed by sequence analysis, we found a 26 kDa spot identical in amino acid sequence to the β6 subunits of the 20S proteasome. No spot of other subunits of 20S proteasome was detected. Here we describe the cloning, sequencing and expression analysis of Carassius auratus, β6.
AB - Proteasomes are large, multisubunit particles that act as the proteolytic machinery for most of the regulated intracellular protein breakdown in eukaryotic cells. Proteasomes are present in both the nucleus and cytoplasm. When we analyzed the molecular composition of protein constituents of the nuclear matrix preparation of goldfish oocytes by two-dimensional polyacrylamide gel electrophoresis followed by sequence analysis, we found a 26 kDa spot identical in amino acid sequence to the β6 subunits of the 20S proteasome. No spot of other subunits of 20S proteasome was detected. Here we describe the cloning, sequencing and expression analysis of Carassius auratus, β6.
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U2 - 10.1016/S0014-5793(00)01441-1
DO - 10.1016/S0014-5793(00)01441-1
M3 - Article
C2 - 10781806
AN - SCOPUS:0034697240
SN - 0014-5793
VL - 472
SP - 62
EP - 66
JO - FEBS Letters
JF - FEBS Letters
IS - 1
ER -