Identification of the human leptin 5'-flanking sequences involved in the trophoblast-specific transcription

Ken Ebihara, Yoshihiro Ogawa, Naohi Isse, Kiyoshi Mori, Naohisa Tamura, Hiroaki Masuzaki, Ken ichi Kohno, Shigeo Yura, Kiminori Hosoda, Norimasa Sagawa, Kazuwa Nakao

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Abstract

Leptin is an adipocyte-derived blood-borne satiety factor that is involved in the regulation of energy homeostasis. We have recently demonstrated nonadipose tissue production of leptin; leptin is synthesized in and secreted from placental trophoblasts. To understand the transcriptional regulation of the human leptin gene in placental trophoblasts, we examined the promoter activity of various lengths of the human leptin 5'-flanking sequences in BeWo cells, a human trophoblastic cell line. The 2080-bp human leptin gene promoter region (-2080 to +108) showed a high-level transcription activity in BeWo cells. When DNA sequences between -1885 and -1830 were deleted, the promoter activity was reduced dramatically in BeWo cells. No significant changes in the promoter activity were noted when tested in primary cultures of rat mature adipocytes. Electrophoretic mobility shift assays revealed the presence of nuclear protein(s) binding to the sequences in BeWo cells but not in isolated rat mature adipocytes. The present study provides new insight into the trophoblast-specific transcription of the human leptin gene.

Original languageEnglish
Pages (from-to)658-663
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume241
Issue number3
DOIs
Publication statusPublished - Dec 29 1997

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Forensic Anthropology
5' Flanking Region
Trophoblasts
Transcription
Leptin
Adipocytes
Genes
Rats
Electrophoretic mobility
DNA sequences
Electrophoretic Mobility Shift Assay
Nuclear Proteins
Genetic Promoter Regions
Protein Binding
Assays
Homeostasis
Blood
Cells
Tissue
Cell Line

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Identification of the human leptin 5'-flanking sequences involved in the trophoblast-specific transcription. / Ebihara, Ken; Ogawa, Yoshihiro; Isse, Naohi; Mori, Kiyoshi; Tamura, Naohisa; Masuzaki, Hiroaki; Kohno, Ken ichi; Yura, Shigeo; Hosoda, Kiminori; Sagawa, Norimasa; Nakao, Kazuwa.

In: Biochemical and Biophysical Research Communications, Vol. 241, No. 3, 29.12.1997, p. 658-663.

Research output: Contribution to journalArticle

Ebihara, K, Ogawa, Y, Isse, N, Mori, K, Tamura, N, Masuzaki, H, Kohno, KI, Yura, S, Hosoda, K, Sagawa, N & Nakao, K 1997, 'Identification of the human leptin 5'-flanking sequences involved in the trophoblast-specific transcription', Biochemical and Biophysical Research Communications, vol. 241, no. 3, pp. 658-663. https://doi.org/10.1006/bbrc.1997.7869
Ebihara, Ken ; Ogawa, Yoshihiro ; Isse, Naohi ; Mori, Kiyoshi ; Tamura, Naohisa ; Masuzaki, Hiroaki ; Kohno, Ken ichi ; Yura, Shigeo ; Hosoda, Kiminori ; Sagawa, Norimasa ; Nakao, Kazuwa. / Identification of the human leptin 5'-flanking sequences involved in the trophoblast-specific transcription. In: Biochemical and Biophysical Research Communications. 1997 ; Vol. 241, No. 3. pp. 658-663.
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