TY - JOUR
T1 - Identification of the lipopolysaccharide core region as the receptor site for a cytotoxin-converting phage, φCTX, of Pseudomonas aeruginosa
AU - Yokota, S. I.
AU - Hayashi, T.
AU - Matsumoto, H.
PY - 1994
Y1 - 1994
N2 - A temperate phage, φCTX, is a cytotoxin-converting phage of Pseudomonas aeruginosa. In this study, we characterized the lipopolysaccharide (LPS) structures of φCTX-resistant mutants derived from φCTX-sensitive strains. φCTX infectivity was neutralized by LPS preparations derived from sensitive strains but not by those from resistant strains. φCTX-resistant mutants had lower-molecular-weight rough (R)-type LPS than the parental strains and lacked the reactivity of some anti-LPS core monoclonal antibodies. Some LPS core components were lacking or significantly decreased in the resistant mutants. These results suggested that a receptor site of the cytotoxin- converting phage φCTX was the LPS core region and that especially L-rhamnose and D-glucose residues in the outer core were involved in phage binding. The host range of φCTX was nearly O-serotype dependent, probably because of the diversity of the LPS core structure among P. aeruginosa strains. φCTX bound to most strains of Homma serotypes A, G, and I but not to strains of serotypes B and E. Furthermore, we found that a genetic locus specifying φCTX sensitivity (and consequently participating in the biosynthesis of part of the LPS core) existed in or near the locus participating in the determination of O-serotype specificity (somA), which has been mapped between leu-10 and eda-9001. φCTX, as well as anti-LPS core monoclonal antibodies, will be a good tool for structural characterization of the P. aeruginosa LPS core region.
AB - A temperate phage, φCTX, is a cytotoxin-converting phage of Pseudomonas aeruginosa. In this study, we characterized the lipopolysaccharide (LPS) structures of φCTX-resistant mutants derived from φCTX-sensitive strains. φCTX infectivity was neutralized by LPS preparations derived from sensitive strains but not by those from resistant strains. φCTX-resistant mutants had lower-molecular-weight rough (R)-type LPS than the parental strains and lacked the reactivity of some anti-LPS core monoclonal antibodies. Some LPS core components were lacking or significantly decreased in the resistant mutants. These results suggested that a receptor site of the cytotoxin- converting phage φCTX was the LPS core region and that especially L-rhamnose and D-glucose residues in the outer core were involved in phage binding. The host range of φCTX was nearly O-serotype dependent, probably because of the diversity of the LPS core structure among P. aeruginosa strains. φCTX bound to most strains of Homma serotypes A, G, and I but not to strains of serotypes B and E. Furthermore, we found that a genetic locus specifying φCTX sensitivity (and consequently participating in the biosynthesis of part of the LPS core) existed in or near the locus participating in the determination of O-serotype specificity (somA), which has been mapped between leu-10 and eda-9001. φCTX, as well as anti-LPS core monoclonal antibodies, will be a good tool for structural characterization of the P. aeruginosa LPS core region.
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U2 - 10.1128/jb.176.17.5262-5269.1994
DO - 10.1128/jb.176.17.5262-5269.1994
M3 - Article
C2 - 8071200
AN - SCOPUS:0028133193
VL - 176
SP - 5262
EP - 5269
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 17
ER -