Identification of the microbiota in carious dentin lesions using 16S rRNA gene sequencing

Junko Obata, Toru Takeshita, Yukie Shibata, Wataru Yamanaka, Masako Unemori, Akifumi Akamine, Yoshihisa Yamashita

Research output: Contribution to journalArticle

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Abstract

While mutans streptococci have long been assumed to be the specific pathogen responsible for human dental caries, the concept of a complex dental caries-associated microbiota has received significant attention in recent years. Molecular analyses revealed the complexity of the microbiota with the predominance of Lactobacillus and Prevotella in carious dentine lesions. However, characterization of the dentin caries-associated microbiota has not been extensively explored in different ethnicities and races. In the present study, the bacterial communities in the carious dentin of Japanese subjects were analyzed comprehensively with molecular approaches using the16S rRNA gene. Carious dentin lesion samples were collected from 32 subjects aged 4-76 years, and the 16S rRNA genes, amplified from the extracted DNA with universal primers, were sequenced with a pyrosequencer. The bacterial composition was classified into clusters I, II, and III according to the relative abundance (high, middle, low) of Lactobacillus. The bacterial composition in cluster II was composed of relatively high proportions of Olsenella and Propionibacterium or subdominated by heterogeneous genera. The bacterial communities in cluster III were characterized by the predominance of Atopobium, Prevotella , or Propionibacterium with Streptococcus or Actinomyces . Some samples in clusters II and III, mainly related to Atopobium and Propionibacterium , were novel combinations of microbiota in carious dentin lesions and may be characteristic of the Japanese population. Clone library analysis revealed that Atopobium sp. HOT-416 and P. acidifaciens were specific species associated with dentinal caries among these genera in a Japanese population. We summarized the bacterial composition of dentinal carious lesions in a Japanese population using next-generation sequencing and found typical Japanese types with Atopobium or Propionibacterium predominating.

Original languageEnglish
Article numbere103712
JournalPloS one
Volume9
Issue number8
DOIs
Publication statusPublished - Aug 1 2014

Fingerprint

Atopobium
Microbiota
Dental Caries
Propionibacterium
rRNA Genes
lesions (animal)
teeth
Genes
ribosomal RNA
Prevotella
dental caries
Chemical analysis
genes
bacterial communities
Olsenella
Lactobacillus
Pathogens
Actinomyces
Streptococcus mutans
Streptococcus

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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Identification of the microbiota in carious dentin lesions using 16S rRNA gene sequencing. / Obata, Junko; Takeshita, Toru; Shibata, Yukie; Yamanaka, Wataru; Unemori, Masako; Akamine, Akifumi; Yamashita, Yoshihisa.

In: PloS one, Vol. 9, No. 8, e103712, 01.08.2014.

Research output: Contribution to journalArticle

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