TY - JOUR
T1 - Identification of the Novel Tooth-Specific Transcription Factor AmeloD
AU - He, B.
AU - Chiba, Y.
AU - Li, H.
AU - de Vega, S.
AU - Tanaka, K.
AU - Yoshizaki, K.
AU - Ishijima, M.
AU - Yuasa, K.
AU - Ishikawa, M.
AU - Rhodes, C.
AU - Sakai, K.
AU - Zhang, P.
AU - Fukumoto, S.
AU - Zhou, X.
AU - Yamada, Y.
N1 - Funding Information:
This work was supported in part by the Intramural Research Program of the National Institute of Dental and Craniofacial Research, National Institutes of Health (Y. Yamada). This work was also supported by the National Natural Science Foundation of China (NSFC81500811 to B. He) and by the Japan Society for the Promotion of Science KAKENHI Grant-in-Aid (15J04116 to Y. Chiba). The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article.
PY - 2019/2/1
Y1 - 2019/2/1
N2 - Basic-helix-loop-helix (bHLH) transcription factors play an important role in various organs’ development; however, a tooth-specific bHLH factor has not been reported. In this study, we identified a novel tooth-specific bHLH transcription factor, which we named AmeloD, by screening a tooth germ complementary DNA (cDNA) library using a yeast 2-hybrid system. AmeloD was mapped onto the mouse chromosome 1q32. Phylogenetic analysis showed that AmeloD belongs to the achaete-scute complex-like (ASCL) gene family and is a homologue of ASCL5. AmeloD was uniquely expressed in the inner enamel epithelium (IEE), but its expression was suppressed after IEE cell differentiation into ameloblasts. Furthermore, AmeloD expression showed an inverse expression pattern with the epithelial cell-specific cell–cell adhesion molecule E-cadherin in the dental epithelium. Overexpression of AmeloD in dental epithelial cell line CLDE cells resulted in E-cadherin suppression. We found that AmeloD bound to E-box cis-regulatory elements in the proximal promoter region of the E-cadherin gene. These results reveal that AmeloD functions as a suppressor of E-cadherin transcription in IEE cells. Our study demonstrated that AmeloD is a novel tooth-specific bHLH transcription factor that may regulate tooth development through the suppression of E-cadherin in IEE cells.
AB - Basic-helix-loop-helix (bHLH) transcription factors play an important role in various organs’ development; however, a tooth-specific bHLH factor has not been reported. In this study, we identified a novel tooth-specific bHLH transcription factor, which we named AmeloD, by screening a tooth germ complementary DNA (cDNA) library using a yeast 2-hybrid system. AmeloD was mapped onto the mouse chromosome 1q32. Phylogenetic analysis showed that AmeloD belongs to the achaete-scute complex-like (ASCL) gene family and is a homologue of ASCL5. AmeloD was uniquely expressed in the inner enamel epithelium (IEE), but its expression was suppressed after IEE cell differentiation into ameloblasts. Furthermore, AmeloD expression showed an inverse expression pattern with the epithelial cell-specific cell–cell adhesion molecule E-cadherin in the dental epithelium. Overexpression of AmeloD in dental epithelial cell line CLDE cells resulted in E-cadherin suppression. We found that AmeloD bound to E-box cis-regulatory elements in the proximal promoter region of the E-cadherin gene. These results reveal that AmeloD functions as a suppressor of E-cadherin transcription in IEE cells. Our study demonstrated that AmeloD is a novel tooth-specific bHLH transcription factor that may regulate tooth development through the suppression of E-cadherin in IEE cells.
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U2 - 10.1177/0022034518808254
DO - 10.1177/0022034518808254
M3 - Article
C2 - 30426815
AN - SCOPUS:85060524374
VL - 98
SP - 234
EP - 241
JO - Journal of Dental Research
JF - Journal of Dental Research
SN - 0022-0345
IS - 2
ER -