Identification of the silkworm quail gene reveals a crucial role of a receptor guanylyl cyclase in larval pigmentation

Masashi Yuasa, Takashi Kiuchi, Yutaka Banno, Susumu Katsuma, Toru Shimada

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Diverse color patterns on the integument of lepidopteran larvae play important roles in their survival through camouflage, mimicry, sexual signaling, and aposematism. In the silkworm Bombyx mori, many color pattern variations have been preserved in inbred strains making them a good model for elucidating the molecular mechanisms that underlie color pattern formation. In this study, we focused on the silkworm quail (q) mutant, which exhibits abnormalities in multiple pigment biosynthesis pathways. Positional cloning of the q gene revealed that disruption of a guanylyl cyclase gene, BmGC-I, is responsible for its abnormal pigmentation. In q mutants, we identified a 16-bp deletion in the BmGC-I transcript, resulting in the production of a premature stop codon. Knockout of the BmGC-I gene resulted in the q-like abnormal pigmentation, thereby demonstrating that the BmGC-I gene is involved in the pigment biosynthesis pathway in the integument. Moreover, quantitative reverse transcription polymerase chain reaction showed that BmGC-I was strongly expressed in the fourth instar on day 2. Our results suggest that BmGC-I deficiency affects the pigment biosynthesis pathway, which supports the involvement of guanylyl cyclase in larval coloration.

Original languageEnglish
Pages (from-to)33-40
Number of pages8
JournalInsect Biochemistry and Molecular Biology
Volume68
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

Guanylate Cyclase-Coupled Receptors
guanylate cyclase
Quail
Bombyx
Pigmentation
quails
silkworms
pigmentation
Biosynthesis
Genes
Pigments
receptors
Color
color
Guanylate Cyclase
pigments
biosynthesis
integument
genes
Camouflage

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Insect Science

Cite this

Identification of the silkworm quail gene reveals a crucial role of a receptor guanylyl cyclase in larval pigmentation. / Yuasa, Masashi; Kiuchi, Takashi; Banno, Yutaka; Katsuma, Susumu; Shimada, Toru.

In: Insect Biochemistry and Molecular Biology, Vol. 68, 01.01.2016, p. 33-40.

Research output: Contribution to journalArticle

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