Identification of the site-specific DNA invertase responsible for the phase variation of SusC/SusD family outer membrane proteins in Bacteroides fragilis

Haruyuki Nakayama-Imaohji, Hideki Hirakawa, Minoru Ichimura, Shin Wakimoto, Satoru Kuhara, Tetsuya Hayashi, Tomomi Kuwahara

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The human gut microbe Bacteroides fragilis can alter the expression of its surface molecules, such as capsular polysaccharides and SusC/SusD family outer membrane proteins, through reversible DNA inversions. We demonstrate here that DNA inversions at 12 invertible regions, including three gene clusters for SusC/SusD family proteins, were controlled by a single tyrosine site-specific recombinase (Tsr0667) encoded by BF0667 in B. fragilis strain YCH46. Genetic disruption of BF0667 diminished or attenuated shufflon-type DNA inversions at all three susC/susD genes clusters, as well as simple DNA inversions at nine other loci, most of which colocalized with susC/susD family genes. The inverted repeat sequences found within the Tsr0667-regulated invertible regions shared the consensus motif sequence AGTYYYN4GDACT. Tsr0667 specifically mediated the DNA inversions of 10 of the 12 regions, even under an Escherichia coli background when the invertible regions were exposed to BF0667 in E. coli cells. Thus, Tsr0667 is an additional globally acting DNA invertase in B. fragilis, which probably involves the selective expression of SusC/SusD family outer membrane proteins.

Original languageEnglish
Pages (from-to)6003-6011
Number of pages9
JournalJournal of bacteriology
Volume191
Issue number19
DOIs
Publication statusPublished - Sep 21 2009

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beta-Fructofuranosidase
Bacteroides fragilis
Membrane Proteins
DNA
Multigene Family
Inverted Repeat Sequences
Escherichia coli
Consensus Sequence
Polysaccharides
Tyrosine
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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Identification of the site-specific DNA invertase responsible for the phase variation of SusC/SusD family outer membrane proteins in Bacteroides fragilis. / Nakayama-Imaohji, Haruyuki; Hirakawa, Hideki; Ichimura, Minoru; Wakimoto, Shin; Kuhara, Satoru; Hayashi, Tetsuya; Kuwahara, Tomomi.

In: Journal of bacteriology, Vol. 191, No. 19, 21.09.2009, p. 6003-6011.

Research output: Contribution to journalArticle

Nakayama-Imaohji, Haruyuki ; Hirakawa, Hideki ; Ichimura, Minoru ; Wakimoto, Shin ; Kuhara, Satoru ; Hayashi, Tetsuya ; Kuwahara, Tomomi. / Identification of the site-specific DNA invertase responsible for the phase variation of SusC/SusD family outer membrane proteins in Bacteroides fragilis. In: Journal of bacteriology. 2009 ; Vol. 191, No. 19. pp. 6003-6011.
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