Abstract
The human gut microbe Bacteroides fragilis can alter the expression of its surface molecules, such as capsular polysaccharides and SusC/SusD family outer membrane proteins, through reversible DNA inversions. We demonstrate here that DNA inversions at 12 invertible regions, including three gene clusters for SusC/SusD family proteins, were controlled by a single tyrosine site-specific recombinase (Tsr0667) encoded by BF0667 in B. fragilis strain YCH46. Genetic disruption of BF0667 diminished or attenuated shufflon-type DNA inversions at all three susC/susD genes clusters, as well as simple DNA inversions at nine other loci, most of which colocalized with susC/susD family genes. The inverted repeat sequences found within the Tsr0667-regulated invertible regions shared the consensus motif sequence AGTYYYN4GDACT. Tsr0667 specifically mediated the DNA inversions of 10 of the 12 regions, even under an Escherichia coli background when the invertible regions were exposed to BF0667 in E. coli cells. Thus, Tsr0667 is an additional globally acting DNA invertase in B. fragilis, which probably involves the selective expression of SusC/SusD family outer membrane proteins.
| Original language | English |
|---|---|
| Pages (from-to) | 6003-6011 |
| Number of pages | 9 |
| Journal | Journal of bacteriology |
| Volume | 191 |
| Issue number | 19 |
| DOIs | |
| Publication status | Published - Sep 21 2009 |
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All Science Journal Classification (ASJC) codes
- Microbiology
- Molecular Biology
Cite this
Identification of the site-specific DNA invertase responsible for the phase variation of SusC/SusD family outer membrane proteins in Bacteroides fragilis. / Nakayama-Imaohji, Haruyuki; Hirakawa, Hideki; Ichimura, Minoru; Wakimoto, Shin; Kuhara, Satoru; Hayashi, Tetsuya; Kuwahara, Tomomi.
In: Journal of bacteriology, Vol. 191, No. 19, 21.09.2009, p. 6003-6011.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of the site-specific DNA invertase responsible for the phase variation of SusC/SusD family outer membrane proteins in Bacteroides fragilis
AU - Nakayama-Imaohji, Haruyuki
AU - Hirakawa, Hideki
AU - Ichimura, Minoru
AU - Wakimoto, Shin
AU - Kuhara, Satoru
AU - Hayashi, Tetsuya
AU - Kuwahara, Tomomi
PY - 2009/9/21
Y1 - 2009/9/21
N2 - The human gut microbe Bacteroides fragilis can alter the expression of its surface molecules, such as capsular polysaccharides and SusC/SusD family outer membrane proteins, through reversible DNA inversions. We demonstrate here that DNA inversions at 12 invertible regions, including three gene clusters for SusC/SusD family proteins, were controlled by a single tyrosine site-specific recombinase (Tsr0667) encoded by BF0667 in B. fragilis strain YCH46. Genetic disruption of BF0667 diminished or attenuated shufflon-type DNA inversions at all three susC/susD genes clusters, as well as simple DNA inversions at nine other loci, most of which colocalized with susC/susD family genes. The inverted repeat sequences found within the Tsr0667-regulated invertible regions shared the consensus motif sequence AGTYYYN4GDACT. Tsr0667 specifically mediated the DNA inversions of 10 of the 12 regions, even under an Escherichia coli background when the invertible regions were exposed to BF0667 in E. coli cells. Thus, Tsr0667 is an additional globally acting DNA invertase in B. fragilis, which probably involves the selective expression of SusC/SusD family outer membrane proteins.
AB - The human gut microbe Bacteroides fragilis can alter the expression of its surface molecules, such as capsular polysaccharides and SusC/SusD family outer membrane proteins, through reversible DNA inversions. We demonstrate here that DNA inversions at 12 invertible regions, including three gene clusters for SusC/SusD family proteins, were controlled by a single tyrosine site-specific recombinase (Tsr0667) encoded by BF0667 in B. fragilis strain YCH46. Genetic disruption of BF0667 diminished or attenuated shufflon-type DNA inversions at all three susC/susD genes clusters, as well as simple DNA inversions at nine other loci, most of which colocalized with susC/susD family genes. The inverted repeat sequences found within the Tsr0667-regulated invertible regions shared the consensus motif sequence AGTYYYN4GDACT. Tsr0667 specifically mediated the DNA inversions of 10 of the 12 regions, even under an Escherichia coli background when the invertible regions were exposed to BF0667 in E. coli cells. Thus, Tsr0667 is an additional globally acting DNA invertase in B. fragilis, which probably involves the selective expression of SusC/SusD family outer membrane proteins.
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UR - http://www.scopus.com/inward/citedby.url?scp=70349132308&partnerID=8YFLogxK
U2 - 10.1128/JB.00687-09
DO - 10.1128/JB.00687-09
M3 - Article
C2 - 19648246
AN - SCOPUS:70349132308
VL - 191
SP - 6003
EP - 6011
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 19
ER -