Identification of three annexin ix isoforms generated by alternative splicing of the carboxyl-terminal exon in silkworm, bombyx mori

Qing You Xia, Hiroshi Fujii, Takahiro Kusakabe, Yutaka Banno

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Annexins (ANXs) are a family of structurally related proteins with Ca2--dependent phospholipid- binding properties. Here we report the cloning of three cDNAs each encoding annexin IX (ANX IX) isoforms from unfertilized eggs of the silkworm, Bombyx mori. The analysis of exon/intron structures showed that the three mRNAs, named ANX IX-A (2300 bp), ANX IX-B (1884 bp) and ANX IX-C (1409 bp), respectively, were generated from a single gene by alternative usage of a 3′-splice site of the last exon. Thus the three isoforms have an identical sequence from amino acid residues 1 to 307 and this region shows approximately 77% identity to Drosophila melanogaster ANX IX. Only amino acid residues 308-324 (A) or 308-323 (B and C), which correspond to the C-terminal tail, are different in the three proteins. A RT-PCR analysis indicated that the three isoforms of silkworm ANX IX were specifically expressed in various larval tissues and development stages. Interestingly, the C-terminal tail in ANXs I, II and V were previously confirmed as a binding region for protein kinase C. Thus generation of the three ANX IX isoforms in the silkworm, that are different from other ANXs, may have a functional significance other than binding to Ca2+.

Original languageEnglish
Pages (from-to)9-14
Number of pages6
JournalInsect Biochemistry and Molecular Biology
Volume32
Issue number1
DOIs
Publication statusPublished - Jan 1 2001

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Insect Science

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