Identification of whey acidic protein (WAP) in Dog Milk

Mami Seki, Rina Matsura, Tokuko Iwamori, Naoko Nukumi, Keitaro Yamanouchi, Kiyoshi Kano, Kunihiko Naito, Hideaki Tojo

Research output: Contribution to journalComment/debate

4 Citations (Scopus)

Abstract

Whey acidic protein (WAP) has been identified as a major whey protein in milk of a wide range of species and reportedly plays important roles in regulating the proliferation of mammary epithelial cells. However, in some species including humans, WAP is not synthesized in the mammary gland. The presence of WAP in carnivore species has not been reported. We searched the National Center for Biotechnology Information (NCBI) database for the dog WAP gene and tried biochemically to identify WAP in dog milk. The nucleotide sequence of the examined dog genomic DNA was completely identical to that in the NCBI database and showed that the dog WAP gene, like other known functional WAP genes, has four exons. Biochemical analysis of milk protein by reverse-phase HPLC and Western blotting demonstrated the presence of WAP in dog milk.

Original languageEnglish
Pages (from-to)67-70
Number of pages4
JournalExperimental Animals
Volume61
Issue number1
DOIs
Publication statusPublished - Jan 1 2012
Externally publishedYes

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whey
Milk
Dogs
milk
dogs
proteins
National Center for Biotechnology Information
Information Centers
Genes
Biotechnology
Databases
genes
whey acidic proteins
Milk Proteins
Human Mammary Glands
whey protein
dairy protein
carnivores
mammary glands
breasts

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

Cite this

Seki, M., Matsura, R., Iwamori, T., Nukumi, N., Yamanouchi, K., Kano, K., ... Tojo, H. (2012). Identification of whey acidic protein (WAP) in Dog Milk. Experimental Animals, 61(1), 67-70. https://doi.org/10.1538/expanim.61.67

Identification of whey acidic protein (WAP) in Dog Milk. / Seki, Mami; Matsura, Rina; Iwamori, Tokuko; Nukumi, Naoko; Yamanouchi, Keitaro; Kano, Kiyoshi; Naito, Kunihiko; Tojo, Hideaki.

In: Experimental Animals, Vol. 61, No. 1, 01.01.2012, p. 67-70.

Research output: Contribution to journalComment/debate

Seki, M, Matsura, R, Iwamori, T, Nukumi, N, Yamanouchi, K, Kano, K, Naito, K & Tojo, H 2012, 'Identification of whey acidic protein (WAP) in Dog Milk', Experimental Animals, vol. 61, no. 1, pp. 67-70. https://doi.org/10.1538/expanim.61.67
Seki M, Matsura R, Iwamori T, Nukumi N, Yamanouchi K, Kano K et al. Identification of whey acidic protein (WAP) in Dog Milk. Experimental Animals. 2012 Jan 1;61(1):67-70. https://doi.org/10.1538/expanim.61.67
Seki, Mami ; Matsura, Rina ; Iwamori, Tokuko ; Nukumi, Naoko ; Yamanouchi, Keitaro ; Kano, Kiyoshi ; Naito, Kunihiko ; Tojo, Hideaki. / Identification of whey acidic protein (WAP) in Dog Milk. In: Experimental Animals. 2012 ; Vol. 61, No. 1. pp. 67-70.
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