TY - JOUR
T1 - Identification of whey acidic protein (WAP) in Dog Milk
AU - Seki, Mami
AU - Matsura, Rina
AU - Iwamori, Tokuko
AU - Nukumi, Naoko
AU - Yamanouchi, Keitaro
AU - Kano, Kiyoshi
AU - Naito, Kunihiko
AU - Tojo, Hideaki
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2012
Y1 - 2012
N2 - Whey acidic protein (WAP) has been identified as a major whey protein in milk of a wide range of species and reportedly plays important roles in regulating the proliferation of mammary epithelial cells. However, in some species including humans, WAP is not synthesized in the mammary gland. The presence of WAP in carnivore species has not been reported. We searched the National Center for Biotechnology Information (NCBI) database for the dog WAP gene and tried biochemically to identify WAP in dog milk. The nucleotide sequence of the examined dog genomic DNA was completely identical to that in the NCBI database and showed that the dog WAP gene, like other known functional WAP genes, has four exons. Biochemical analysis of milk protein by reverse-phase HPLC and Western blotting demonstrated the presence of WAP in dog milk.
AB - Whey acidic protein (WAP) has been identified as a major whey protein in milk of a wide range of species and reportedly plays important roles in regulating the proliferation of mammary epithelial cells. However, in some species including humans, WAP is not synthesized in the mammary gland. The presence of WAP in carnivore species has not been reported. We searched the National Center for Biotechnology Information (NCBI) database for the dog WAP gene and tried biochemically to identify WAP in dog milk. The nucleotide sequence of the examined dog genomic DNA was completely identical to that in the NCBI database and showed that the dog WAP gene, like other known functional WAP genes, has four exons. Biochemical analysis of milk protein by reverse-phase HPLC and Western blotting demonstrated the presence of WAP in dog milk.
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U2 - 10.1538/expanim.61.67
DO - 10.1538/expanim.61.67
M3 - Comment/debate
C2 - 22293674
AN - SCOPUS:84856601453
SN - 1341-1357
VL - 61
SP - 67
EP - 70
JO - Experimental Animals
JF - Experimental Animals
IS - 1
ER -