Identifying a sigma class glutathione S-transferase 2 from the silkworm Bombyx mori

Aiko Hirowatari, Sumiharu Nagaoka, Naotaka Yamada, Kohji Yamamoto

Research output: Contribution to journalArticle

Abstract

A cDNA that encodes a sigma class glutathione S-transferase (GST; bmGSTS2) from the silkworm (Bombyx mori) was cloned by reverse transcriptase polymerase chain reaction and sequenced. The deduced amino acid sequence revealed 68%-63% identity with the sigma class GSTs from other insects. bmGSTS2 mRNA was widely distributed in various tissues. The recombinant enzyme was functionally overexpressed as a soluble form in Escherichia coli, purified to homogeneity, and characterized. The optimum pH of bmGSTS2 was approximately pH 7.0, and bmGSTS2 retained >75% of its original activity after incubation for 12 h at pH 6.0-8.0. Incubation for 30 min at temperatures below 40°C did not affect the enzymatic activity. bmGSTS2 was able to catalyze the reaction of glutathione with hydrogen peroxide, and 4-hydroxynonenal. These results indicate that bmGSTS2 may play a role in antioxidant defense in the silkworm.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalJournal of Insect Biotechnology and Sericology
Volume86
Issue number1
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Bombyx
Bombyx mori
silkworms
Glutathione Transferase
glutathione transferase
Polymerase chain reaction
Antioxidants
Hydrogen peroxide
Escherichia coli
Amino acids
Enzymes
Tissue
Reverse Transcriptase Polymerase Chain Reaction
Hydrogen Peroxide
Glutathione
hydrogen peroxide
Insects
glutathione
Amino Acid Sequence
amino acid sequences

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Business, Management and Accounting(all)
  • Agricultural and Biological Sciences(all)
  • Insect Science
  • Industrial and Manufacturing Engineering

Cite this

Identifying a sigma class glutathione S-transferase 2 from the silkworm Bombyx mori. / Hirowatari, Aiko; Nagaoka, Sumiharu; Yamada, Naotaka; Yamamoto, Kohji.

In: Journal of Insect Biotechnology and Sericology, Vol. 86, No. 1, 01.01.2017, p. 1-7.

Research output: Contribution to journalArticle

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