Abstract
DNA methylation is a stable epigenetic modification, which is well known to be involved in gene expression regulation. In general, however, analyzing DNA methylation requires rather time consuming processes (24–96 h) via DNA replication and protein modification. Here we demonstrate a methodology to analyze DNA methylation at a single DNA molecule level without any protein modifications by measuring the contracted length and relaxation time of DNA within a nanochannel. Our methodology is based on the fact that methylation makes DNA molecules stiffer, resulting in a longer contracted length and a longer relaxation time (a slower contraction rate). The present methodology offers a promising way to identify DNA methylation without any protein modification at a single DNA molecule level within 2 h.
| Original language | English |
|---|---|
| Pages (from-to) | 644-649 |
| Number of pages | 6 |
| Journal | Science and Technology of Advanced Materials |
| Volume | 17 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2016 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Materials Science(all)
Cite this
Identifying DNA methylation in a nanochannel. / Sun, Xiaoyin; Yasui, Takao; Yanagida, Takeshi; Kaji, Noritada; Rahong, Sakon; Kanai, Masaki; Nagashima, Kazuki; Kawai, Tomoji; Baba, Yoshinobu.
In: Science and Technology of Advanced Materials, Vol. 17, No. 1, 01.01.2016, p. 644-649.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identifying DNA methylation in a nanochannel
AU - Sun, Xiaoyin
AU - Yasui, Takao
AU - Yanagida, Takeshi
AU - Kaji, Noritada
AU - Rahong, Sakon
AU - Kanai, Masaki
AU - Nagashima, Kazuki
AU - Kawai, Tomoji
AU - Baba, Yoshinobu
PY - 2016/1/1
Y1 - 2016/1/1
N2 - DNA methylation is a stable epigenetic modification, which is well known to be involved in gene expression regulation. In general, however, analyzing DNA methylation requires rather time consuming processes (24–96 h) via DNA replication and protein modification. Here we demonstrate a methodology to analyze DNA methylation at a single DNA molecule level without any protein modifications by measuring the contracted length and relaxation time of DNA within a nanochannel. Our methodology is based on the fact that methylation makes DNA molecules stiffer, resulting in a longer contracted length and a longer relaxation time (a slower contraction rate). The present methodology offers a promising way to identify DNA methylation without any protein modification at a single DNA molecule level within 2 h.
AB - DNA methylation is a stable epigenetic modification, which is well known to be involved in gene expression regulation. In general, however, analyzing DNA methylation requires rather time consuming processes (24–96 h) via DNA replication and protein modification. Here we demonstrate a methodology to analyze DNA methylation at a single DNA molecule level without any protein modifications by measuring the contracted length and relaxation time of DNA within a nanochannel. Our methodology is based on the fact that methylation makes DNA molecules stiffer, resulting in a longer contracted length and a longer relaxation time (a slower contraction rate). The present methodology offers a promising way to identify DNA methylation without any protein modification at a single DNA molecule level within 2 h.
UR - http://www.scopus.com/inward/record.url?scp=85019142000&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85019142000&partnerID=8YFLogxK
U2 - 10.1080/14686996.2016.1223516
DO - 10.1080/14686996.2016.1223516
M3 - Article
VL - 17
SP - 644
EP - 649
JO - Science and Technology of Advanced Materials
JF - Science and Technology of Advanced Materials
SN - 1468-6996
IS - 1
ER -