TY - JOUR
T1 - Identifying DNA methylation in a nanochannel
AU - Sun, Xiaoyin
AU - Yasui, Takao
AU - Yanagida, Takeshi
AU - Kaji, Noritada
AU - Rahong, Sakon
AU - Kanai, Masaki
AU - Nagashima, Kazuki
AU - Kawai, Tomoji
AU - Baba, Yoshinobu
N1 - Funding Information:
This research was supported by the Cabinet Office, Government of Japan and the Japan Society for the Promotion of Science (JSPS) through the Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program), the ImPACT Program of the Council for Science, Technology and Innovation (Cabinet Office, Government of Japan), Nanotechnology Platform Program (Molecule and Material Synthesis) of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan, the JSPS Grant-in-Aid for Scientific Research (A) [16H02091], and the Grant-in-Aid for Scientific Research on Innovative Areas ?Nanomedicine Molecular Science? [number 26107709] from the MEXT and PRESTO, Japan Science and Technology Agency (JST).
Publisher Copyright:
© 2016 The Author(s). Published by National Institute for Materials Science in partnership with Taylor & Francis.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - DNA methylation is a stable epigenetic modification, which is well known to be involved in gene expression regulation. In general, however, analyzing DNA methylation requires rather time consuming processes (24–96 h) via DNA replication and protein modification. Here we demonstrate a methodology to analyze DNA methylation at a single DNA molecule level without any protein modifications by measuring the contracted length and relaxation time of DNA within a nanochannel. Our methodology is based on the fact that methylation makes DNA molecules stiffer, resulting in a longer contracted length and a longer relaxation time (a slower contraction rate). The present methodology offers a promising way to identify DNA methylation without any protein modification at a single DNA molecule level within 2 h.
AB - DNA methylation is a stable epigenetic modification, which is well known to be involved in gene expression regulation. In general, however, analyzing DNA methylation requires rather time consuming processes (24–96 h) via DNA replication and protein modification. Here we demonstrate a methodology to analyze DNA methylation at a single DNA molecule level without any protein modifications by measuring the contracted length and relaxation time of DNA within a nanochannel. Our methodology is based on the fact that methylation makes DNA molecules stiffer, resulting in a longer contracted length and a longer relaxation time (a slower contraction rate). The present methodology offers a promising way to identify DNA methylation without any protein modification at a single DNA molecule level within 2 h.
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U2 - 10.1080/14686996.2016.1223516
DO - 10.1080/14686996.2016.1223516
M3 - Article
AN - SCOPUS:85019142000
VL - 17
SP - 644
EP - 649
JO - Science and Technology of Advanced Materials
JF - Science and Technology of Advanced Materials
SN - 1468-6996
IS - 1
ER -