Identifying DNA methylation in a nanochannel

Xiaoyin Sun, Takao Yasui, Takeshi Yanagida, Noritada Kaji, Sakon Rahong, Masaki Kanai, Kazuki Nagashima, Tomoji Kawai, Yoshinobu Baba

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

DNA methylation is a stable epigenetic modification, which is well known to be involved in gene expression regulation. In general, however, analyzing DNA methylation requires rather time consuming processes (24–96 h) via DNA replication and protein modification. Here we demonstrate a methodology to analyze DNA methylation at a single DNA molecule level without any protein modifications by measuring the contracted length and relaxation time of DNA within a nanochannel. Our methodology is based on the fact that methylation makes DNA molecules stiffer, resulting in a longer contracted length and a longer relaxation time (a slower contraction rate). The present methodology offers a promising way to identify DNA methylation without any protein modification at a single DNA molecule level within 2 h.

Original languageEnglish
Pages (from-to)644-649
Number of pages6
JournalScience and Technology of Advanced Materials
Volume17
Issue number1
DOIs
Publication statusPublished - Jan 1 2016

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DNA
Proteins
Relaxation time
Molecules
Gene expression regulation
Methylation
DNA Methylation

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

Identifying DNA methylation in a nanochannel. / Sun, Xiaoyin; Yasui, Takao; Yanagida, Takeshi; Kaji, Noritada; Rahong, Sakon; Kanai, Masaki; Nagashima, Kazuki; Kawai, Tomoji; Baba, Yoshinobu.

In: Science and Technology of Advanced Materials, Vol. 17, No. 1, 01.01.2016, p. 644-649.

Research output: Contribution to journalArticle

Sun, Xiaoyin ; Yasui, Takao ; Yanagida, Takeshi ; Kaji, Noritada ; Rahong, Sakon ; Kanai, Masaki ; Nagashima, Kazuki ; Kawai, Tomoji ; Baba, Yoshinobu. / Identifying DNA methylation in a nanochannel. In: Science and Technology of Advanced Materials. 2016 ; Vol. 17, No. 1. pp. 644-649.
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