@inbook{f02e955a7b484586aad2795fd50ab360,
title = "Ideonella sakaiensis, PETase, and MHETase: From identification of microbial PET degradation to enzyme characterization",
abstract = "Few reports have described the biological degradation or utilization of poly(ethylene terephthalate) (PET) to support microbial growth. We screened environmental samples from a PET bottle recycling site and identified the microbial consortium no. 46, which degraded amorphous PET at ambient temperature; thereafter, we isolated the resident Ideonella sakaiensis 201-F6 strain responsible for the degradation. We further identified two hydrolytic enzymes from I. sakaiensis, PET hydrolase (PETase) and mono(2-hydroxyethyl) terephthalate hydrolase (MHETase), which synergistically converted PET into its monomeric building blocks. Here, we provide original methods of microbial screening and isolation of PET degrading microbe(s). These novel approaches can be adapted for exploring microorganisms that degrade PET and other plastics. Furthermore, our enzyme assay protocols to characterize PETase and MHETase can be applied to evaluate new enzymes that target PET and its hydrolysates.",
author = "Shosuke Yoshida and Kazumi Hiraga and Ikuo Taniguchi and Kohei Oda",
note = "Funding Information: We thank A. Wlodawer for critical comments regarding this manuscript. This study was supported by grants-in-aid for scientific research (17H03794 to S.Y.). The nucleotide sequence data are available in the DNA Data Bank of Japan, European Molecular Biology Laboratory, and GenBank databases under the accession numbers BBYR01000001 to BBYR01000227 BBYR01000001, BBYR01000227. Ideonella sakaiensis 201-F6T has been deposited as strains NBRC 110686T and TISTR 2288T. None. Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",
year = "2021",
month = jan,
doi = "10.1016/bs.mie.2020.12.007",
language = "English",
isbn = "9780128220122",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "187--205",
editor = "Gert Weber and Bornscheuer, {Uwe T.} and Ren Wei",
booktitle = "Enzymatic Plastic Degradation",
address = "United States",
}