IFT-81 and IFT-74 are required for intraflagellar transport in C. elegans

Tetsuo Kobayashi, Keiko Gengyo-ando, Takeshi Ishihara, Isao Katsura, Shohei Mitani

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Intraflagellar transport (IFT) is essential machinery for biogenesis and maintenance of cilia in many eukaryotic and prokaryotic cells. A large number of polypeptides are known to be involved in IFT, but the physiological role of each component is not fully elucidated. Here, we identified a C. elegans orthologue of a Chlamydomonas reinhardtii IFT component, IFT-81, and found that its loss-of-function mutants show an unusual behavioral property and small body size. IFT-81 is expressed in sensory neurons, and localized at the base of cilia. The similar phenotypes with ift-81 mutants were also observed in several IFT mutants, suggesting these defects are caused by inability of IFT. We also demonstrated that IFT-81 interacts and co-localizes with IFT-74, which is another putative component of IFT. The ift-74 loss-of-function mutants showed phenocopies with ift-81 mutants, suggesting IFT-81 and IFT-74 play comparable functions. Moreover, ift-81 and ift-74 mutants similarly exhibited weak anomalies in cilia formation and obvious disruptions of transport in mature cilia. Thus, we conclude that IFT-81 and IFT-74 coordinately act in IFT in C. elegans sensory cilia.

Original languageEnglish
Pages (from-to)593-602
Number of pages10
JournalGenes to Cells
Volume12
Issue number5
DOIs
Publication statusPublished - May 1 2007

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Cilia
Prokaryotic Cells
Chlamydomonas reinhardtii
Body Size
Eukaryotic Cells
Sensory Receptor Cells
Maintenance
Phenotype
Peptides

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

IFT-81 and IFT-74 are required for intraflagellar transport in C. elegans. / Kobayashi, Tetsuo; Gengyo-ando, Keiko; Ishihara, Takeshi; Katsura, Isao; Mitani, Shohei.

In: Genes to Cells, Vol. 12, No. 5, 01.05.2007, p. 593-602.

Research output: Contribution to journalArticle

Kobayashi, Tetsuo ; Gengyo-ando, Keiko ; Ishihara, Takeshi ; Katsura, Isao ; Mitani, Shohei. / IFT-81 and IFT-74 are required for intraflagellar transport in C. elegans. In: Genes to Cells. 2007 ; Vol. 12, No. 5. pp. 593-602.
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