IgA protease from Neisseria gonorrhoeae inhibits exocytosis in bovine chromaffin cells like tetanus toxin

T. Binscheck, F. Bartels, H. Bergel, H. Bigalke, S. Yamasaki, T. Hayashi, H. Niemann, J. Pohlner

Research output: Contribution to journalArticle

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Abstract

When tetanus toxin from Clostridium tetani or IgA protease from Neisseria gonorrhoeae is translocated artificially into the cytosol of chromaffin cells, both enzymes inhibit calcium-induced exocytosis, which can be measured by changes in membrane capacitance. The block of exocytosis caused by both proteases cannot be reversed by enforced stimulation with increased calcium concentration. This effect differs from the botulinum A neurotoxin-induced block of exocytosis that can be overcome by elevation of the intracellular calcium concentration. Tetanus toxin is about 50-fold more potent than IgA protease in cells stimulated by carbachol. In this case, the release of [3H]noradrenaline was determined. Trypsin and endoprotease Glu-C are hardly effective and only at concentrations that disturb the integrity of the cells. Like tetanus toxin, IgA protease also splits synaptobrevin II, though at a different site of the molecule. However, unlike tetanus toxin, it does not cleave cellubrevin. It is concluded that the membranes of chromaffin vesicles contain synaptobrevin II, which, as in neurons, appears to play a crucial part in exocytosis.

Original languageEnglish
Pages (from-to)1770-1774
Number of pages5
JournalJournal of Biological Chemistry
Volume270
Issue number4
DOIs
Publication statusPublished - Jan 1 1995

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IgA-specific serine endopeptidase
Tetanus Toxin
Chromaffin Cells
Neisseria gonorrhoeae
Exocytosis
R-SNARE Proteins
Calcium
Vesicle-Associated Membrane Protein 3
Membranes
Neurotoxins
Carbachol
Cytosol
Trypsin
Neurons
Norepinephrine
Peptide Hydrolases
Capacitance

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

IgA protease from Neisseria gonorrhoeae inhibits exocytosis in bovine chromaffin cells like tetanus toxin. / Binscheck, T.; Bartels, F.; Bergel, H.; Bigalke, H.; Yamasaki, S.; Hayashi, T.; Niemann, H.; Pohlner, J.

In: Journal of Biological Chemistry, Vol. 270, No. 4, 01.01.1995, p. 1770-1774.

Research output: Contribution to journalArticle

Binscheck, T, Bartels, F, Bergel, H, Bigalke, H, Yamasaki, S, Hayashi, T, Niemann, H & Pohlner, J 1995, 'IgA protease from Neisseria gonorrhoeae inhibits exocytosis in bovine chromaffin cells like tetanus toxin', Journal of Biological Chemistry, vol. 270, no. 4, pp. 1770-1774. https://doi.org/10.1074/jbc.270.4.1770
Binscheck, T. ; Bartels, F. ; Bergel, H. ; Bigalke, H. ; Yamasaki, S. ; Hayashi, T. ; Niemann, H. ; Pohlner, J. / IgA protease from Neisseria gonorrhoeae inhibits exocytosis in bovine chromaffin cells like tetanus toxin. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 4. pp. 1770-1774.
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